...cytometry (“spectral flow”) has emerged as a particularly useful tool for those wishing to run larger ...Mayo Clinic studying host-microbe interactions in early life.    Resources More resources on the Addgene...

...expression cassette in one single viral particle. The earliest explorations of the use of AdV for in vivo CRISPR-Cas...of AdV-modified human cells being subjected to scarless homology-directed genome editing (Maggio et al...

...seen some major advances in light microscopy, particularly related to the neurosciences.  These developments...tetrahydrofuran-based tissue clearing (13,14). A few years later, Karl Deisseroth’s lab developed CLARITY, which is a hydrogel-based...

...The maturation of the chromophore on many FPs (particularly those derived from GFP) requires oxygen. Therefore...interested in everything related to gene expression, particularly at the RNA level. He maintains the greenfluorescentblog.wordpress.com...

...didn’t attend. For example, we got a good bit of early feedback from those who wanted, but were unable ...carefully organized. Most importantly, they should be regularly assessed for continued relevance and if needed...

...experiences. STEM in elementary schools - catching them early I’m fortunate to live in a town with a good public...means it was the best kind of networking! Since nearly every talk I’ve given in the past 20 years has ...

...information while using less resources and are particularly useful when trying to screen a limited number...discriminate between non-essential and essential genes similarly to the Brunello knock-out library while mitigating...

...conjugate, and the complexity of the system. A particularly desirable property for small-molecule labeling...removing excess dye from the sample, which is particularly important for samples where washing is difficult...

...mere millimeters apart. Similarly, cells compact DNA in a way that brings linearly distant regions close...

...Lentiviral vector history and components In the early 1990s, researchers developed viral vector systems... but this research is still in the pre-clinical/early clinical stages. Researchers are also working to...

...reprogramming from 0.001% to nearly 0.1% [7]. In terms of hiPSC colonies, nearly 16 hiPSC colonies can now...

... your technical knowledge and judgement. For particularly tricky cases, team discussions can often lead...doing a lot of science communication, and is particularly excited to hear about novel developments in ...

...What is an aptamer? Nearly 30 years ago, two independent groups, led by Jack Szostak and Larry Gold, ... Assays that require recognition of ions or particularly small molecules that do not generate an immune...

...article is an interactive chart where you can see the yearly interdisciplinarity index of research for single...and case study." Journal of Librarianship and Scholarly Communication 2.2 (2014): 2. Additional Resources...

...major effect on scientists within academia, most particularly postdocs working in the U.S., whose current ...thing. Reliance on a cheap trainee workforce has clearly not been a sustainable approach to biomedical research...

... Figure 4: Reporters in Gene On include a mScarlet reporter, luciferase reporter, and neomycin resistance...translation of an array of different reporters including mScarlet-I, luciferase, antibiotic resistance markers, ...

...CRISPR Cas variant that can also cleave dsDNA similarly to Cas9 (Zetsche et al., 2015 ). Cas12a however...DETECTR. How does DETECTR work? DETECTR works similarly to SHERLOCK. Cas12a is targeted to a specific ...

...conferences should strive to provide support to early career scientists, students, and those from historically...Juliet Johnston, Kat Milligan-Myhre, Kim Dill-McFarland, Lisa Gorski, Mark O. Martin, Maureen Berg, Michael...

...and I met scientists from 21 different labs who clearly also share this wonder of the organism.   It’s...songs like humans learn speech. Finches must learn early, use auditory feedback to learn, babble as baby ...

...segmentation during embryogenesis. Typically, regularly spaced denticles (bristles) outline the segments... of cookie monster mutant flies are filled with early mitotic cells as well as necrotic or dead cells ...