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We narrowed to 671 results for: FIE

Showing: 261 - 280 of 671 results

  1. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...Cas9 is revolutionizing the mouse gene-targeting field. Mice have long been extremely useful in the lab...components, a guide RNA (gRNA) of about 125 nt that specifies the target, the Cas9 endonuclease that creates...Z, Huang J (2016) Efficient Production of Gene-Modified Mice using Staphylococcus aureus Cas9. Sci Rep...

  2. Important Considerations When Using AAVs

    Type
    Blog Post
    ...AAV2 & AAV6 Astrocytes AAV8 > AAV2 & AAV6 Modified from Aschauer et al. (2013) What does this mean...distance traveled, etc.) (4). This has also been identified with AAV2 (5), AAV6 (6) and AAV5 at excitatory... many experiments, a group of researchers has identified this feature of AAVs as a new tool for neuroanatomical...

  3. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

    Type
    Blog Post
    ... DNA you want to edit; however, BAC DNA can be modified with any of the below described approaches.    ...red recombination event. Alternatively, if the modified allele is selectable, it can be transferred to... chromosome. After this, mini-λ can readily be purified from bacteria just like a plasmid. Phage A final...

  4. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    ...part? Less complicated math. Bioengineering is a field that has been gaining a lot of traction in recent... end_module_attribute %}{% module_attribute "field_types" is_json="true" %}{% raw %}{"button_text":"text...end_module_attribute %}{% module_attribute "wrap_field_tag" is_json="true" %}{% raw %}"div"{% endraw %...

  5. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ...strange and exciting revelation. Science in any field can generally be applied to improve our lives and...those scholarships that you don’t think you’re qualified for. Wayne Gretzky’s “You miss 100% of the shots...and ended up with the award. We are often more qualified and capable than we know or believe we are. Funded...

  6. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...facilitate other lentivirus- and AAV-based studies. Unified MoClo Systems While many MoClo kits are designed...behavior of an expression construct. Once you’ve identified your experimental requirements, it’s a good idea...j.xgen.2024.100519. Chiasson, David, et al. “A Unified Multi-Kingdom Golden Gate Cloning Platform.” Scientific...

  7. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ... highlight some recent advancements within this field. Fundamental principles of fluorescent dyes/ligands... by autofluorescence (Lucidi et al., 2023). The field of fluorescent ligands and dyes has witnessed remarkable...Purington, E., Gramlich, W., Grondin, Y., Rogers, R., Bousfield, D., & Demokritou, P. (2019). Development & characterization...

  9. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...647 secondary antibodies. Antibody staining was quantified using the Attune NxT Flow Cytometer with representative...with a screening procedure in easily genetically modified cell types (top). The specific approach will depend...References Ayoubi, R., Ryan, J., Biddle, M. S., Alshafie, W., Fotouhi, M., Bolivar, S. G., Ruiz Moleon,...

  11. Trends in CRISPR and SynBio Technologies

    Type
    Blog Post
    ...deposits and distirbution for these fast moving fields.  Addgene is a global nonprofit plasmid repository...proud of the role we are playing in advancing this field and how we are able to support rapid progress in...

  12. Protocol - Over-Agar Antibiotic Plating

    Type
    Protocol
    ...to a plate after it's already been poured and solidified..... With a 6 cm diameter petri plate containing solidified LB-agar, pipette 150 µL of carbenicillin on top...

  13. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...past two decades, several virus species have been modified and used as tools for tracing. With some engineering...a starting point for future developments in the field of AAV vectors. Conclusion An ideal comprehensive...AAV vectors could also be powerful tools for the field of gene therapy if they can reach the affected part...

  14. Expanded Access to Rabies Plasmids

    Type
    Blog Post
    ...rabies and all other lyssa genus members to the specified international institution. Requesters will need...and may not be required for others. You will be notified if an export license is not required for your ...

  18. Science Communication for Everyone

    Type
    Blog Post
    ...mathematicians. We hope to highlight every science field as we expand and encourage more direct conversations... work with you? We welcome researchers from all fields of science to share their experiences and explain...

  19. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    ...genome editing in human cells. The Feng Zhang lab identified a Cas12b from Bacillus hisashii, BhCas12b that...a screening approach, the Jennifer Doudna lab identified three different Cas12a inhibitors. Some of these...

  20. Your Top Requested Plasmid in 2016!

    Type
    Blog Post
    ...pyogenes (Sp) Cas9 from a Cbh promoter. Cbh is a modified version of CAG promoter that the Zhang lab has...transfect a variety of mammalian cells and can be modified for viral trasduction (see the Zhang Lab's Nature...
Showing: 261 - 280 of 671 results