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We narrowed to 671 results for: FIE

Showing: 261 - 280 of 671 results
  1. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    ...part? Less complicated math. Bioengineering is a field that has been gaining a lot of traction in recent... end_module_attribute %}{% module_attribute "field_types" is_json="true" %}{% raw %}{"button_text":"text...end_module_attribute %}{% module_attribute "wrap_field_tag" is_json="true" %}{% raw %}"div"{% endraw %...
  2. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ... highlight some recent advancements within this field. Fundamental principles of fluorescent dyes/ligands... by autofluorescence (Lucidi et al., 2023). The field of fluorescent ligands and dyes has witnessed remarkable...Purington, E., Gramlich, W., Grondin, Y., Rogers, R., Bousfield, D., & Demokritou, P. (2019). Development & characterization...
  3. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...facilitate other lentivirus- and AAV-based studies. Unified MoClo Systems While many MoClo kits are designed...behavior of an expression construct. Once you’ve identified your experimental requirements, it’s a good idea...j.xgen.2024.100519. Chiasson, David, et al. “A Unified Multi-Kingdom Golden Gate Cloning Platform.” Scientific...
  4. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ...647 secondary antibodies. Antibody staining was quantified using the Attune NxT Flow Cytometer with representative...with a screening procedure in easily genetically modified cell types (top). The specific approach will depend...References Ayoubi, R., Ryan, J., Biddle, M. S., Alshafie, W., Fotouhi, M., Bolivar, S. G., Ruiz Moleon,...
  5. Protocol - Over-Agar Antibiotic Plating

    Type
    Protocol
    ...to a plate after it's already been poured and solidified..... With a 6 cm diameter petri plate containing solidified LB-agar, pipette 150 µL of carbenicillin on top...
  6. Trends in CRISPR and SynBio Technologies

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    Blog Post
    ...deposits and distirbution for these fast moving fields.  Addgene is a global nonprofit plasmid repository...proud of the role we are playing in advancing this field and how we are able to support rapid progress in...
  7. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...past two decades, several virus species have been modified and used as tools for tracing. With some engineering...a starting point for future developments in the field of AAV vectors. Conclusion An ideal comprehensive...AAV vectors could also be powerful tools for the field of gene therapy if they can reach the affected part...
  8. Expanded Access to Rabies Plasmids

    Type
    Blog Post
    ...rabies and all other lyssa genus members to the specified international institution. Requesters will need...and may not be required for others. You will be notified if an export license is not required for your ...
  9. Lentivirus Production

    Type
    Protocol
    ...) transfection protocol. This procedure can be modified for alternative packaging cell lines or transfection...without limiting cell growth. Left panels: bright field images; right panels: GFP channel images. Last reviewed...
  10. Your Top Requested Plasmid in 2016!

    Type
    Blog Post
    ...pyogenes (Sp) Cas9 from a Cbh promoter. Cbh is a modified version of CAG promoter that the Zhang lab has...transfect a variety of mammalian cells and can be modified for viral trasduction (see the Zhang Lab's Nature...
  11. Science Communication for Everyone

    Type
    Blog Post
    ...mathematicians. We hope to highlight every science field as we expand and encourage more direct conversations... work with you? We welcome researchers from all fields of science to share their experiences and explain...
  12. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    ...genome editing in human cells. The Feng Zhang lab identified a Cas12b from Bacillus hisashii, BhCas12b that...a screening approach, the Jennifer Doudna lab identified three different Cas12a inhibitors. Some of these...
  13. Developing Transferable Skills During Science Training

    Type
    Blog Post
    ...references. Collaborate with another lab, in another field maybe, and publish Join a lab that has multiple ...committee) Follow industry publications like FierceBiotech to “learn the business” Attend events with local...
  14. Let There Be LITE Plasmids

    Type
    Blog Post
    ... via epigenetics with the addition of histone modifiers. “We want to allow people to prove the causal ...August 2013).   , with the addition of histone modifiers, ...
  15. Transfection for Recombinant Antibodies

    Type
    Protocol
    ...and expression, the recombinant antibody can be purified for use in a variety of applications. Sharing ... used as-is or the recombinant antibody can be purified from the supernatant (see our Recombinant Antibody...
Showing: 261 - 280 of 671 results