We narrowed to 402 results for: Mut

1. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

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   ... assays. ShadowG is darker than other dark YFP mutants, it reduces the risk of spectral contamination ...

2. Plasmids for Endogenous Gene Tagging in Human Cells

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   ...it’s also good to make a few single base pair mutations in crRNA target sites found within the homology...

3. Mouse Modeling, Part 1: Genetically Engineered Mice

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   ...deletions, but CRISPR/Cas9 can also introduce point mutations. While using targeted nucleases to generate an...

4. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

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   ...now circulating and plasmids containing these mutations have been deposited at Addgene. Plasmids deposited...

5. The Materials Science of Optogenetics Experiments

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   ...to give the animal the full range of motion, a commutator allows the patch cable to connect to the laser...

6. Google Forums Round Up: First Impressions of NgAgo

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   ...wouldn’t be a surprise if someone came up with a mutant NgAgo (or a novel endonuclease) protein that can...

7. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

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   ... and Citrine) can be monomerized with an A206K mutation [2]. Addgene has many of these constructs and ...

8. Anatomy of a Plasmid Page at Addgene

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   ...depositor has indicated that there is a particular mutation found within the gene/insert, this information...

9. 3D Printing Meets CRISPR Cas9

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   ... this model also displays the several Lys/Ala mutations reported by Ian Slaymaker and colleagues to reduce...

10. Truncated gRNAs for Regulating Gene Expression

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    ... gene expression. Its catalytic domains can be mutated to inactivate the protein’s nucleolytic capability...

11. Plasmids 101: Knockout/Knock-In Plasmids

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    ...possible to insert, or knock-in, any gene, tag, or mutated exon into the genome. For this purpose, the sequence...

12. Degrading DNA with Cascade-Cas3

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    ...chance of a chromosomal inversion, or just random mutations if the chromosome is repaired with all the original...

13. Pooled Library Amplifications

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    ...Screening pCMV-Rep78/68 Scanning Saturation Mutagenesis (SSM) Pooled Library 80,962 1866 bp, plus...

14. Plasmids 101: Multicistronic Vectors

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    ...should also note that IRES and 2A peptides are not mutually exclusive elements. Labs have successfully utilized...

15. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

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    ...tools, double dimerization GFPs, and circular permutation GFPs. Hopefully we will have new tools for plant...

16. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

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    ...gates." Science. 340, 599-603 (3 May 2013). Vivek Mutalik et al. "Precise and reliable gene expression via...

17. Lentiviral Vector Uses and Overview

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    ...transfer plasmids. The packaging plasmid carried a mutated HIV-1 provirus that couldn’t package itself due...

18. CRISPR Antimicrobials

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    ...defective phagemid; Bikard et al. did not observe any mutations at the target site. In a mouse skin colonization...

19. Tips for arabidopsis transformation

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    ...and proteins in living plants, to complementing mutant phenotypes. Arabidopsis is amenable to the floral...

20. Transferable Skills Guide: Identifying Your Transferable Skills

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    ...lucky socks, persevered through a nerve wracking commute complete with a disabled train, and now you’re ...