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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9 results for: Mut

Showing: 1 - 9 of 9 results

  1. CRISPR Guide

    Type
    Guide
    ...developed by mutating specific amino acid residues to reduce off-target editing. Some mutations disrupt interactions...off-target mutations in DNA, RNA, or both, and are recommended in contexts where such mutations would be... and a gRNA will result in a diverse array of mutations (see: Validate genetic modification ). In most...amino acid deletions, insertions, or frameshift mutations leading to premature stop codons within the open...targeted gene. The ideal result is a loss-of-function mutation within the targeted gene. However, the strength...strength of the knockout phenotype for a given mutant cell must be validated experimentally. Browse Plasmids...(nick) or no cuts. Cas9 nickase (Cas9n), a D10A mutant of SpCas9, has one active nuclease domain and one...

  2. Optogenetics Guide

    Type
    Guide
    ...improve these natural opsins - by inducing point mutations to alter the absorption spectrum or adding trafficking... variants have been created via genetic point mutations, codon optimization, and chimeric fusion of domains...photocurrent compared to CrChR2 450 ChETA E123T mutation; creates faster kinetics but reduces photocurrent...photocurrent amplitude 490 ChR/T159C T159C mutation; displays increased photocurrents 470 SFO/SSFO Step function...function Opsins and Stabilized Step Function Opsins. Mutations at C128 and D156 of CrChR delay the closing of...Chlamydomonas noctigama . ChrimsonR carries K176R mutation. 590 PsChR2 High-efficiency blue-shifted channelrhodopsin... C1V1 ChR1-VChR1 chimera with E122T and E162T mutations 540 Channelrhodopsins: chloride channels iChloC...

  3. Plan Your Experiment

    Type
    Guide
    ...(knockout)? Generate a specific mutant allele of a gene (point mutant)? Increase or decrease expression...Furthermore, in cells containing two mutated alleles, each mutated allele may be different owing to the...many different types of edits, including point mutations, base changes, and large insertions or deletions...) Generate specific genomic edits, like point mutations or small tag insertions, by copying from a DNA...Generate targeted insertions, deletions, and point mutations using a prime editing guide RNA (pegRNA) Prime... alternative splicing, and an early frameshift mutation is more likely to result in a non-functional protein...base editing , or prime editing experiments, most mutated alleles will not contain the desired edit. Even...

  4. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ... create and test capsid libraries with random mutations or insertions in the Cap region. Several methods...immune response, and the low risk of insertional mutagenesis. Several AAV-based gene therapies have been approved...chromosome, which reduces the risk of insertional mutagenesis. However, random integration events can occur...host organism, leading to disease. Insertional mutagenesis Random insertion of DNA elements into the genome...material into cells. Replication-competent rAAV Mutated rAAV vector that has the ability to replicate and...

  5. Lentiviral Vector Guide

    Type
    Guide
    ...harnessed for stable delivery of various genes, mutations, or treatments into cells, and has been widely... the laboratory, and many have been removed or mutated for increased safety. Figure 1: Wild-type lentivirus...biological processes by surveying a pool of genetic mutants for various phenotypes. These screens involve hundreds...used to create transgenic mice with heritable mutations or transgene expression. Popular uses include ...

  6. Chemogenetics Guide

    Type
    Guide
    ...? blog! Studies of GPCRs led to development of mutated receptors that while unresponsive to endogenous...PSEMs). PSAM domains were first named for the mutations that allowed them to respond to their cognate ...anti-smoking drug varenicline. This PSAM carries mutations at L131G, Q139L, and Y217F and is termed PSAM4...

  7. Adenovirus Guide

    Type
    Guide
    ...highly reduces the potential for insertional mutagenesis and the risk of oncogenesis. However, random ... signals of infection or damage. Insertional mutagenesis Random insertion of DNA elements into the genome... cells. Replication-competent adenovirus (RCA) Mutated adenoviral vector that has the ability to replicate...

  8. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...harnessed for stable delivery of various genes, mutations, or treatments into cells, and has been widely...

  9. Molecular Biology Reference

    Type
    Guide
    ... gene function as well as identify changes or mutations that may cause certain diseases. Sequencing DNA...
Showing: 1 - 9 of 9 results