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We narrowed to 72 results for: Mut

Showing: 1 - 20 of 72 results

  1. Biosensor AAV Preps

    Type
    Collection
    ...Constitutive 9 Li 140555 pAAV-hsyn-GRAB_DA-mut Syn GRAB_DA-mut (control) none Constitutive 9 Li 140556 pAAV-hsyn-GRAB_rDA1m...Constitutive 9 Li 140558 pAAV-hsyn-GRAB_rDA-mut Syn GRAB_rDA-mut (control) none Constitutive 9 Li 208702 ...Constitutive 9, rg* Li 123310 pAAV-hSyn-GRAB_NEmut Syn GRAB_NEmut (control) none Constitutive 9, rg* Li ...Constitutive 1, 9 Li 185388 pAAV-hSyn-GRAB_OTmut Syn GRAB_OTmut (control) none Constitutive 1, 9 Li Voltage...

  2. Brain Initiative Collection

    Type
    Collection
    ...AAV9 pAAV-hsyn-GRAB_DA-mut Expresses the DA-insensitive control sensor GRAB_DA-mut in neurons Yulong Li ...pAAV-hsyn-GRAB_rDA-mut Expresses the red fluorescent DA-insensitive control sensor GRAB_rDA-mut in neurons Yulong...NE) control sensor GRAB_NEmut in neurons Yulong Li 123310-AAVrg pAAV-hSyn-GRAB_NEmut Expresses the genetically-encoded...GRAB_NE1h in neurons Yulong Li 123310-AAV9 pAAV-hSyn-GRAB_NEmut Expresses the genetically-encoded fluorescent...fluorescent norepinephrine(NE) control sensor GRAB_NEmut in neurons Yulong Li 125560-AAV1 pAAV-CAG-dLight1.3b...Cre-dependent AAV expression of humanized ChR2 (with H134R mutation) fused to eYFP for optogenetic activation Viviana...

  3. Open Enzyme Collection

    Type
    Collection
    ...Addgene ID Plasmid Gene/insert 165546 pOpen-MMLV_RT (mut H) Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase... Transcriptase (RNAse H deactivated by 3 mutations) 165556 pOpen-MMLV_RT (lack H) Moloney Murine Leukemia...

  4. Genetic Code Expansion

    Type
    Collection
    ...changes to UAG, RF1 function removed, MutS restored so decreased mutation rate George Church 68306 C321.ΔA ... UAG codons changed to UAA. mutS+, λ-, ΔybhB-bioAB, ΔprfA, ΔserB mutations for improved yield and growth...endogenous TAG codons mutated to TAA, RF1 (prfA) deleted, fabR spontaneously mutated, serB deleted for phosphoserine...jannaschii , M. barkeri , or E.coli and can be mutated and screened through directed evolution to charge.... mazei Mammalian TAG Ivana Nikić-Spiegel 182881 Mut1-RS tyrosyl-tRNA synthetase M. jannaschii 4-propargyloxy-l-phenylalanine...propargyloxy-l-phenylalanine (pPR) Bacterial TAG Miriam Amiram 182882 Mut2-RS tyrosyl-tRNA synthetase M. jannaschii 4-propargyloxy-l-phenylalanine...

  6. Rett Syndrome

    Type
    Collection
    ...caused from loss-of-function mutations in the MECP2 gene. Causative mutations for Rett syndrome typically...within patients with the same mutation, the location and type of MECP2 mutation can be a strong predictor ...missense mutations located in the MBD, and truncations prior to the NID are more severe than mutations further...to their laboratory website. Cell Line Mutation (DNA) Mutation (protein) Sex Source of Material Publication...species (mouse or human) Mutation ID Plasmid Description Gene/Insert Mutations PI Return to top External...meet diagnostic criteria have disease-causing mutations in the gene methyl-CpG binding protein 2 ( MECP2... expressing wild-type MECP2 and half expressing mutant MECP2 . Skewing of these X inactivation ratios ...

  7. p53 Pathway

    Type
    Collection
    ...frequently mutated gene (>50%) in human cancer; most of these mutations are missense mutations in the DNA-binding...hotspot residues. These mutations can be inherited or arise through exposure to mutagens like radiation or ...PubMed PMID: 24379683 . When mutants gain new powers: news from the mutant p53 field. Brosh R, Rotter V...19776742 . Germline TP53 mutations and Li-Fraumeni syndrome. Varley JM. Hum Mutat. 2003 Mar;21(3):313-20... (e.g., HPV, the human papillomavirus). These mutations interfere with p53’s ability to activate transcription...Li-Fraumeni syndrome. Recently some impacts of p53 mutations have been characterized as gain-of-function, expanding... expanding the mechanisms by which mutant p53 is known to function in cancer. p53 Pathway Plasmids Click...

  8. CRISPR Guide

    Type
    Collection
    ...developed by mutating specific amino acid residues to reduce off-target editing. Some mutations disrupt interactions...off-target mutations in DNA, RNA, or both, and are recommended in contexts where such mutations would be... and a gRNA will result in a diverse array of mutations (see: Validate genetic modification ). In most...amino acid deletions, insertions, or frameshift mutations leading to premature stop codons within the open...targeted gene. The ideal result is a loss-of-function mutation within the targeted gene. However, the strength...strength of the knockout phenotype for a given mutant cell must be validated experimentally. Browse Plasmids...(nick) or no cuts. Cas9 nickase (Cas9n), a D10A mutant of SpCas9, has one active nuclease domain and one...

  9. University of Florida Serotype Testing Panel for the Eye and Brain

    Type
    Collection
    ...contains the following mutations: Y444F, Y500F, and Y730F. AAV2(4pMut)dHS The AAV2(4pMut)dHS serotype displays...21):6229-39. PMID: 26310623 AAV2(4pMut)dHS When using the AAV2(4pMut)dHS serotype in future publications...spread following injection in the brain. The AAV2(4pMut)dHS serotype was developed by Shannon Boye’s lab...It is derived from AAV2 and has the following mutations: Y444F, Y500F, Y730F, T491V, R487G, R585S, and...from the AAV6 capsid and contains the following mutations: Y705F, Y731F and T492V. AAV44.9 The AAV44.9 serotype...High-efficiency transduction of the mouse retina by tyrosine-mutant AAV serotype vectors. Mol Ther . 2009 Mar;17(3)...citations include: Bogner, et al. 2015. Capsid Mutated Adeno-Associated Virus Delivered to the Anterior...

  10. Plan Your Experiment

    Type
    Collection
    ...(knockout)? Generate a specific mutant allele of a gene (point mutant)? Increase or decrease expression...Furthermore, in cells containing two mutated alleles, each mutated allele may be different owing to the...many different types of edits, including point mutations, base changes, and large insertions or deletions...) Generate specific genomic edits, like point mutations or small tag insertions, by copying from a DNA...Generate targeted insertions, deletions, and point mutations using a prime editing guide RNA (pegRNA) Prime... alternative splicing, and an early frameshift mutation is more likely to result in a non-functional protein...base editing , or prime editing experiments, most mutated alleles will not contain the desired edit. Even...

  11. Deisseroth INTRSECT Collection

    Type
    Collection
    ...Recombinases Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral Items 55636 pAAV-EF1a-Cre None ...-dependent Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral Items 55641 pAAV-Ef1a-fDIO EYFP...Fluorophores Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral Items 55650 pAAV-hSyn Con/Fon EYFP...recombinase-dependent: GECI Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral Items 137119 pAAV-EF1a-Con/Fon-...Excitatory Opsins Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral Items 55645 pAAV-hSyn Con/Fon hChR2...Inhibitory Opsins Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral Items 137148 pAAV-nEF-Con/Fon-Arch3.3...recombinase-dependent: DREADDs Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral Items 177669 pAAV-nEF-Coff/Fon ...

  12. COVID-19 Resources

    Type
    Collection
    ...) Ectodomain and RBD mutants that can be used to identify antibody escape mutants and for comprehensive...Ectodomain and RBD mutants. SARS-CoV-2 Spike Receptor Binding Domain Deep Mutation Scanning Library Screening...Screening Jesse Bloom Library of Spike protein RBD mutations for comprehensive mapping. SARS-CoV-2 Spike Receptor...Receptor Binding Domain Site-Saturation Mutagenesis Libraries Screening Jesse Bloom Tyler Starr Libraries...Libraries of variant Spike protein RBD mutations for comprehensive mapping. Other Resources Research Articles...

  13. Malate Dehydrogenase CUREs Community Collection

    Type
    Collection
    ...glyoxysomal, mitochondrial, peroxisomal) Mutation (specific mutation, "active site", "active site loop", or...-type malate dehydrogenase genes and watermelon mutant clones for your course-based undergraduate research...single protein with many available isoforms and mutants provides a shared experience for the research community...of wild-type MDH genes from several species and mutant clones from watermelon have been codon-optimized...interface") ID Plasmid Description Gene/Insert Mutations Additional Resources MDH CUREs Community (Link...

  14. Bacterial Expression Systems

    Type
    Collection
    ...Promoter activity GUS activity and fluorescence (GFPmut3.1) Gram-negative bacteria Philip Poole 14471 pRU1144...14462 pRU1097 Promoter activity Fluorescence (GFPmut3.1) Gram-negative bacteria Philip Poole 14461 pRU1701...14083 pAKgfplux1 Promoter activity Fluorescence (GFPmut3a) and luminescence (lux operon) Gram-negative bacteria... 14076 pAKgfp1 Promoter activity Fluorescence (GFPmut3a) Gram-negative bacteria Attila Karsi 18855 FLIParaF.Ec... Mattheos Koffas 173481 HC-M ATP Fluorescence (GFPmut2) Escherichia coli Rahul Sarpeshkar 111614 pCdrA-gfpC...pCdrA-gfpC Cyclic di-GMP Fluorescence (GFPmut2) Pseudomonas aeruginosa Tim Tolker-Nielsen 90217 pMV762-Peredox-mCherry...by knocking out genes or introducing specific mutations can reveal important insights into gene function...

  15. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ...somatic hypermutation in a 100 bp window. This technique creates diverse populations of mutants for directed... create A->G (or T->C on the opposite strand) mutations. CRISPR-X, a base-editing system from the Bassik... Marker PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted single-strand breaks in...created by wild type Cas enzymes. To use a nickase mutant, you will need two gRNAs that target opposite strands...strategies reduce unwanted off-target effects. Nickase mutants can also be used with a repair template to introduce...

  16. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Izaurralde 147272 pAc5.1B-lambdaN-HA-DmeIF4Ga-4Ebind-mut-dsRNAres_Q EIF4G His, HA, V5 Ac5 Parkinson's Elisa...17798 pM-ErbB4CTF-PY1 mutant ERBB4 GAL4-BD ALS Marius Sudol 17799 pM-ErbB4CTF-PY3 mutant ERBB4 GAL4-BD ALS...delta-kinase-PY1 mutant ERBB4 GAL4-BD ALS Marius Sudol 17802 pM-ErbB4-delta-kinase-PY3 mutant ERBB4 GAL4-BD...below can be sorted by disease, plasmid type, mutations, and/or tags such as fluorescent protein (FP) ...Disease Parkinson's Disease Others Gene Type Wildtype Mutant Partial Sequence Codon-Optimized gRNA Other Tags... YFP GAL1 ALS Aaron Gitler 29608 426Gal-FUS-RRM Mutant-YFP FUS YFP GAL1 ALS Aaron Gitler 29609 pcDNA 3.2...TMEM106B_Halo_C_allele TMEM106B Halo Frontotemporal dementia in GRN mutation carriers Michael Ward 178174 VAPB_Halo_C_allele...

  17. Cancer Research Plasmids and Resources

    Type
    Collection
    ... for use in cancer signaling studies, including mutant, knockdown, and overexpression constructs. Each...growth of the cell. p53 p53, the most frequently mutated gene in human cancer, is a transcription factor...Meyerson , and Root Labs containing wild-type and mutant alleles described in the initial Target Accelerator...vectors for in vitro and in vivo research. TP53 Mutagenesis Library : Pooled library from the Hahn and Root...labs for characterization of all possible p53 mutations. Resources New Tool for Lineage Tracing: The ClonTracer...

  18. AAV for Neuronal Tracing

    Type
    Collection
    ...pAAV-synP-FLEX-splitTVA-EGFP-B19G Can be used to complement deletion-mutant rabies virus AAV1 Ian Wickersham 100798 pAAV-syn-FLEX-splitTVA-EGFP-tTA...AAV together can be used to complement deletion-mutant rabies virus AAV1 Ian Wickersham 100799 pAAV-TREtight-mTagBFP2...the cell population of interest with a deletion-mutant tracing virus that lacks the ability to infect ... To enable monosynaptic spread of this deletion-mutant rabies, the essential G protein is provided to ...2007a. Retrograde neuronal tracing with a deletion-mutant rabies virus. Nat Methods. 4(1):47-9. PMID: 17179932...

  19. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ... protein component of MMR) mutant to inhibit MMR PE5 - PE3 plus MLH1 mutant to inhibit MMR PEmax - optimized...installing targeted insertions, deletions, and point mutations using a prime editing guide RNA (pegRNA). As with...tools are available: PE2 - introduction of five mutations in RT enzyme PE3 - PE2 plus additional sgRNA PE4...additional nuclear localization signals, and two mutations in Cas9 to enhance nuclease activity; can be used...

  20. TALEN Plasmids and Kits

    Type
    Collection
    ...truncated at both the N and C terminus and induces mutation at rates much higher than the parental vectors...generation architecture has been shown to increase mutation induction when using TALENs. The FokI domains ...homodimeric or enhanced heterodimeric (ELD, KKR mutations) FokI domains. 40131 pCAG-T7-TALEN(Sangamo)-FokI-KKR-Destination... has successfully been employed for deletion mutagenesis and gene targeting in human and mouse pluripotent...suitable for the TALEN-based generation of germline mutations in Bombyx mori and Drosophila melanogaster . Zhang...
Showing: 1 - 20 of 72 results