...mismatch/mutation may be the result of a mis-called peak in the trace file. If the mutation is not an...such as the gene/insert, fusion proteins, point mutations, deletions, etc.) involves selecting one or more...Addgene sequences the plasmid to verify tags, mutations and a portion of the insert, but we do not sequence...

...use a high fidelity taq polymerase to minimize mutations. The fidelity of the polymerase becomes more important...PCR based cloning carries a much higher risk for mutation than restriction enzyme based cloning. DNA replication...

...making a small change in a plasmid (such as point mutations). Generate DNA segments by PCR. Run PCR product...

...ultraviolet (UV) light. Caution EtBr is a known mutagen. Wear a lab coat, eye protection and gloves when...

...cations can also be used for PCR-mediated DNA mutagenesis. A higher cation concentration increases the ...