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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 5 results for: Mut

Showing: 1 - 5 of 5 results

  1. Protocol - How to Perform Sequence Analysis

    Type
    Protocol
    ...mismatch/mutation may be the result of a mis-called peak in the trace file. If the mutation is not an...such as the gene/insert, fusion proteins, point mutations, deletions, etc.) involves selecting one or more...Addgene sequences the plasmid to verify tags, mutations and a portion of the insert, but we do not sequence...

  2. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ...use a high fidelity taq polymerase to minimize mutations. The fidelity of the polymerase becomes more important...PCR based cloning carries a much higher risk for mutation than restriction enzyme based cloning. DNA replication...

  3. Gibson Assembly Protocol

    Type
    Protocol
    ...making a small change in a plasmid (such as point mutations). Generate DNA segments by PCR. Run PCR product...
Showing: 1 - 5 of 5 results