We narrowed to 619 results for: PROC
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TypeBlog Post...make a transgenic fly in order to study it. The process is fairly straightforward. Basically, you place... incorporated into the germline. To begin this process, you will first need to do some molecular cloning...
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Targeted Mutagenesis with EvolvR
TypeBlog Post... the nick site, which was expected since the processivity window of PolI is 15-20 bp. EvolvR’s on-target...bacteriophage T7 DNA polymerase increased the processivity of PolI3M, and thereby increasing the editing... -
Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors
TypeBlog Post...wrong scenarios” and making sure we have adequate process and controls in place to manage those scenarios...Plan's risk management, control, and governance processes. Prior to Tufts Health Plan, Carl worked at both... -
Viral Vectors 101: Biosensors
TypeBlog Post...factors as proxies for a cellular process or directly measure a cellular process. Cellular phenomena such as... -
Plasmids 101: Screening Strategies Used in Plasmid Cloning
TypeBlog Post...analyze plasmids, which can help you visualize this process, or visit our restriction analysis blog post. ...now use next-generation sequencing during our QC process. This way, we can confirm the sequence of the entire... -
Behind the scenes: Addgene’s new search engine and more
TypeBlog Post...quickly. Through the years, we have perfected processes to curate the data our depositors share and enrich...and handle millions of queries. In an iterative process driven by our user experience experts, our software... -
Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools
TypeBlog Post...uses far less toxic biotin for labeling, but the process is much slower and can take as long as several ...-protein interactions and mediate biochemical processes. One of the largest and best characterized of ... -
Plasmids 101: Methylation and Restriction Enzymes
TypeBlog Post...protein expression. Methylases involved with these processes (for example Dam and Dcm methylases) are independent...used for site directed mutagenesis. During this process, incorporation of a desired mutation into your ... -
3 Challenges in Plant Synthetic Biology
TypeBlog Post...understand, and the complicated and unique metabolic processes of plants are being investigated on limited funding... developmental, metabolic, and photosynthetic processes. Often the tools developed for plant molecular... -
Kiran Musunuru on the Newest TALEN Genome-Editing System
TypeBlog Post... see how those mutations influence the disease process. In the Cell Stem Cell paper, for example, we generated... increase the efficiency of the genome editing process. The first really popular tool was the zinc finger... -
Addgene: The First Twenty Years
TypeBlog Post...meant facilitating the Material Transfer Agreement process, which was a major barrier for depositors and requestors... requestors alike. We transformed this process by aligning institutions with standardized agreements and... -
Transferable Skills Guide: Public Speaking
TypeBlog Post...your ideas, invite others into decision making processes, and even interview better! As a scientist turned..., re-oriented, continued presenting. The whole process took just under a minute. I can't say the journal... -
Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute
TypeBlog Post...creative. “You need a lot of time to let you mind process things. Sometimes finding that uninterrupted time...about being a writer that can be a very painful process. And certain stories can make you feel like ‘I’... -
Antibodies 101: Selecting the Right Antibody
TypeBlog Post...you with some advice on the antibody selection process. Antibodies are not one size fits all When choosing...experiments are the same. Differences in sample processing, fixation, antigen-retrieval, and permeabilization... -
Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards
TypeBlog Post...vital for researchers to better understand the processes that happen inside the cell,” says Pedre Pérez...from our extremities. “Here, painful stimuli are processed by a network of excitatory and inhibitory neurons... -
Plasmids 101: CcdB - The Toxic Key to Efficient Cloning
TypeBlog Post...still express ccdB and therefore would die. This procedure dramatically reduces the number of clones that...recombinant plasmid and therefore makes the cloning process much more efficient, as one does not have to thoroughly... -
Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening
TypeBlog Post... massively parallel combinatorial genetics." Proceedings of the National Academy of Sciences 111.34 (2014... CRISPR-Cas9 screening enabled by CombiGEM." Proceedings of the National Academy of Sciences113.9 (2016... -
New Tool for Lineage Tracing: The ClonTracer Library
TypeBlog Post... she could “get exposure to the drug discovery process.” The ClonTracer library originally came about ...that these are selected for during the treatment process. The authors went on to show that combination treatment... -
Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing
TypeBlog Post...that could influence the results? The peer-review process is not perfect, so even though it is science’s ...There are a few different ways to automate the process as well. Sign up for eTOC (electronic table of ... -
Working with Nuclear Receptors
TypeBlog Post...self-propagation by reproduction. Both physiological processes are controlled by deliberate and compulsory actions...goals is to promote an understanding of how these processes are regulated at the cellular, tissue and organ...