We narrowed to 494 results for: control
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TypeBlog Post...1998). Regulatory elements within the ori also control copy number and stability, which influence the ...which consist of a plasmid’s ori and all of its control elements, from broad host range plasmids can be...
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Deep Mutational Scanning with One Pot Saturation Mutagenesis
TypeBlog Post...favoring of certain types of mutations, difficulty controlling the rate of mutagenesis, and low throughput. ...combination of library coverage, mutational efficiency, control over where mutations are introduced, and scalability... -
CRISPR Activation: A Practical Guide
TypeBlog Post...Weissman JS (2014) Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation. Cell 159:647...2015) A light-inducible CRISPR-Cas9 system for control of endogenous gene activation. Nat Chem Biol 11... -
Getting the Most from Your Lentiviral Transduction
TypeBlog Post...approach complete with freely available positive controls. Routinely test your cell line for mycoplasma ...Cleghorn G., West M.J., Wei MQ. “The significance of controlled conditions in lentiviral vector titration and... -
More Data for You: Find Articles Citing Addgene Plasmids
TypeBlog Post... 101: How to Verify Your Plasmid Plasmids 101: Control Plasmids 6 Tips for Analyzing and Troubleshooting... -
500,000 Plasmids Shipped and Counting!
TypeBlog Post...your plasmids, storing them, providing quality control and customer support, making plasmids easy for ... -
Optogenetics AAV Preps
TypeCollection...Optogenetics AAV Optogenetic tools can be used to control cell activity in living tissue with light. Optogenetics...Optogenetics is typically used in neuroscience to control electrical potentials in neurons. See our Optogenetics... -
Recombinases AAV Preps
TypeCollection...site-specific recombinases, which can be used to control gene expression. See our Cre-lox Plasmids page ... -
CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More
TypeBlog Post... of knockdown and developed new, more tightly controlled, Tet responsive promoters to avoid leaky activation...This approach provides a more granular level of control where individual bases can be modified. Cytidine...as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression. Cell 152:1173–1183 . https:... -
CRISPR Guide
TypeCollection...-occurring Acr (Anti-CRISPR) proteins can help control CRISPR activity. Acr proteins have evolved in phages...LmoCas9 and SpCas9. Acr proteins can be used to control both when and where genome editing happens. CASANOVA...Dominik Niopek’s lab , is a way to optogenetically control genome editing by fusing the anti-CRISPR protein...photosensitive LOV2 domain . Several labs have also controlled Acr activity with endogenous cell-type specific...have also been used in in vivo RNA imaging or to control alternative splicing. Figure 13: Overview of RNA...Engineered anti-CRISPR proteins for optogenetic control of CRISPR–Cas9. Nature Methods , 15 (11), 924–927...homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery. eLife . 3 e04766... -
What's New in CRISPR - May 2019
TypeBlog Post...significant hit across the E. coli genome as a negative control. Read the paper in Nature Communications Find... -
What's New in CRISPR - Spring 2019
TypeBlog Post...has the advantage that CRISPR efficiency can be controlled by manipulating complementarity between the gRNA... -
How Exercise Purges Stress and Keeps Depression at Bay
TypeBlog Post...noises and flashing lights - that sent average, control mice into depression. In case you were wondering... -
Designer PUF Proteins for Any RNA Target
TypeBlog Post...circuits, allowing for tighter spatio-temporal control of gene expression within a cell. Thanks to the... -
AAV Molecular Tools
TypeCollection... These AAV encode tetracycline-inducible tools/controls and tetracycline transactivators that can be used... -
Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?
TypeBlog Post...with the lentivirus HIV-1. Today, HIV-1 can be controlled with antiviral therapies such that the virus ...infection period rather than the ART-induced viral control found in most HIV-1 patients. Obstacles for CRISPR... -
Modulate the Activity of 17 Signaling Pathways with One Kit!
TypeBlog Post... they do so by activating signaling pathways controlling properties like growth, survival, and differentiation... -
The 10 Most Distributed Plasmid Technologies in Addgene's First 10 Years
TypeBlog Post...TOPFlash, scramble shRNA for use as a negative control, and empty backbones such as pBABE-puro for retroviral... -
Antibody Plasmid Collection
TypeCollection...undergo application-specific validation and quality control by Addgene as well as by our trusted partner labs... -
Distribution to Industry
TypeCollection...undergo application-specific validation and quality control by Addgene as well as by our trusted partner labs...