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Showing: 261 - 280 of 484 results

  4. Editor's Choice, July 2016

    Type
    Blog Post
    ...example of a FRET experiment with all relevent controls near the end of the post also really helped put...

  7. Antibody Plasmid Collection

    Type
    Collection
    ...undergo application-specific validation and quality control by Addgene as well as by our trusted partner labs...

  8. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...Technology Austria, decided to take a novel approach to control for these effects, drawing on the rapidly growing...also available at Addgene and can be used as a control for your experiments. For more information, please... allowing labs to precisely and non-invasively control neuronal signaling. For more information on DREADDs...viral (AAV) system enables greater spatio-temporal control over expression and increases the number of species...Thy1 or CAG promoter, while Brainbow AAV is under control of the EF1a promoter. Livet et al., Nature. 2007...

  11. The Pleiades Promoter Project

    Type
    Collection
    ...MiniPromoter Source Gene Construct Reporter Negative Control N/A pEMS1312 cre N/A pEMS1301 cre/EGFP/NLS N/A ...EGFP/NLS N/A pEMS1313 intron-lacZ/NLS Positive Control CAG promoter pEMS1293 cre CAG promoter pEMS1164...

  12. Molecular Biology Reference

    Type
    Guide
    ...that the antibiotic resistance gene is under the control of a bacterial promoter, and is thus expressed ...strength of the promoter is also important for controlling the level of insert expression (i.e., a strong...antibiotic resistance gene (this time, under the control of a non-bacterial promoter) or a fluorescent protein...plasmids are sequenced with NGS during our quality control process . NGS allows us to sequence entire plasmids...

  13. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ... direct shipments weren’t able to clear import control. Partnering with a distributor allowed us to share... systems to allow for easier access to export-controlled items by streamlining permissions and paperwork...

  14. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ...a large number of requests for vectors in the “controls” category, which includes things like viral vectors...Patrick, M., Guerin, K., & Fan, M. (2023). Quality control for Adeno-associated viral vector production. Neuromethods...

  15. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ...1998). Regulatory elements within the ori also control copy number and stability, which influence the ...which consist of a plasmid’s ori and all of its control elements, from broad host range plasmids can be...

  16. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    ...favoring of certain types of mutations, difficulty controlling the rate of mutagenesis, and low throughput. ...combination of library coverage, mutational efficiency, control over where mutations are introduced, and scalability...

  19. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...Weissman JS (2014) Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation. Cell 159:647...2015) A light-inducible CRISPR-Cas9 system for control of endogenous gene activation. Nat Chem Biol 11...

  20. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    ...approach complete with freely available positive controls. Routinely test your cell line for mycoplasma ...Cleghorn G., West M.J., Wei MQ. “The significance of controlled conditions in lentiviral vector titration and...
Showing: 261 - 280 of 484 results