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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 328 results for: hal.1

Showing: 261 - 280 of 328 results

  1. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    ... if the name was the correct one!)    Fig. 1: HeLa cells. Image courtesy of Josef Reischig.  ...split you got from the graduate student down the hall who got it from some technician downstairs who got...

  2. CRISPR in the Clinic

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    Blog Post
    ...significant progress in clinical trials.     Figure 1: Types of delivery and editing strategies for CRISPR...Disease (SCD) and Transfusion-dependent beta thalassemia (TBT), both of which affect hemoglobin. Most ... enzymes target several strains of E. coli and lethally chop their genomes into many, many pieces. Locus...technology is rapidly evolving, and many of the challenges with therapies involve patient safety, effectiveness...

  3. INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons

    Type
    Blog Post
    ... end sequences (R and L ends).  Figure 1: (A) Components involved in each type of CRISPR-Transposon...proteins to form the Cascade targeting complex is a hallmark of Type I CRISPR-Cas systems, in contrast to single-protein...with the transposition protein TniQ for targeting (Halpin-Healy et al., 2020). Genomic targets require an...creates a 5-bp target-site duplication (TSD), a hallmark characteristic of the Tn7-family of transposons...follow him on twitter @Vopinator.     References: Halpin-Healy TS, Klompe SE, Sternberg SH, Fernández IS...doi.org/10.1038/s41586-019-1849-0 Klompe SE, Vo PLH, Halpin-Healy TS, Sternberg SH (2019) Transposon-encoded...

  5. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    ...updated version of BRET called BRET2. Figure 1: TRUPATH measures heterotrimeric G protein dissociation...constructs, localization of the Rluc8 was more challenging. The Roth lab used structure-guided protein engineering...

  6. 10 Ways to Share Your Science!

    Type
    Blog Post
    ...them you can do today! 10 ways to share your science 1)   If you don’t already make it a habit to talk – ...   Consider participating in a future SciFund Challenge. While the goal is ostensibly to get some funding...

  7. New Viral Vectors - Winter 2025

    Type
    Blog Post
    ...then scroll on for some wintery delights!    Table 1: New ready-to-use viral vectors in the Addgene repository...Depositor  Notes pAAV.CAGFLEX.(cyto).iATPSnFR2.S29W.A95K.HaloTag AAV5 Biosensor Tim Brown New viral prep ...

  8. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ... successful postdoc fellowship. 1. Reverse engineer success On Day 1 of your postdoctoral experience,...Today's postdoctoral fellows (PFs) face a number of challenges ranging from long periods of training to limited...

  9. CASTing Off for New Shores in Human Genome Editing

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    Blog Post
    ...large sequences and even entire genes. Figure 1: Mechanism of CAST systems. A Cas effector (Cascade...s41596-023-00927-3 Klompe, S. E., Vo, P. L. H., Halpin-Healy, T. S., & Sternberg, S. H. (2019). Transposon-encoded...

  11. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type
    Blog Post
    ...trans-activator Brainbow) (Sakaguchi et al., 2018) (Figure 1). Once introduced, fluorescent proteins were best ...with containing chemical tags such as the SNAP-tag, Halo-tag, and CLIP-tag (Kohl et al., 2014) (Addgene #104106...

  13. Antibodies 101: Epitope Tags

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    Blog Post
    ...net negative charge with 4 negatively charged and 1 positively charged amino acids. Myc tags are frequently...doesn’t come with a high background can be a huge challenge. Epitope tags eliminate the need for target-specific...

  14. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...vesicles. Okada CY, Rechsteiner M. Cell. 1982 May;29(1):33-41. PubMed. Protein transduction: unrestricted...targeting a gene associated with diphtheria toxin lethality and were sorted into single wells to generate ...

  15. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    ...Transcriptional Programs with CRISPR RNA Scaffolds. Cell, 160(1), 339-350 (2015). PubMed. Find the Plasmids @Addgene...However, these approaches are often met with the challenges of regulatory bottlenecks and their scope is ...

  16. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    ... After all I was only going to be interviewed for 1 hour so having a broad enough insight was going to...was researching, but I decided I needed a new challenge. Looking for sales and marketing jobs Like most...

  18. Hot Plasmids - October 2020

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    Blog Post
    ... inhibitory synapses in the brain.  Figure 1:Schematic diagram of different FingR constructs and...synaptic connections and physiology is particularly challenging as they are great in number, generally very small...H2B-FT-red is long lasting and was shown to have a half life of 84 hours in primary mouse embryonic fibroblasts...

  19. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    ...treatment and improving patients lives. References 1. Ong-Abdullah, Meilina, et al. "Loss of Karma transposon...real-time, posing a huge challenge to studying epigenetic changes over time.  This challenge was difficult to ...

  20. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type
    Blog Post
    ...supervisor, even if he or she is a difficult person: 1) Understand your supervisor’s expectations Every ... of your supervisor’s management style, bring challenges to his or her attention as soon as you can. It...mutually beneficial solutions. If you package your challenges with your supervisor into learning opportunities...
Showing: 261 - 280 of 328 results