Genome-wide Screening Using CRISPR
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.... Current Protocols in Molecular Biology 115: . https://doi.org/10.1002/cpmb.8
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Antibodies 101: Producing Recombinant Antibodies
TypeBlog Post..., Issue 5, 2014, Pages 438-449, ISSN 1359-0294, https://doi.org/10.1016/j.cocis.2014.10.002. Randolph ... -
What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry
TypeBlog Post...Expression Newsletter. Issue 15/10. March 2010. https://www.qiagen.com/de/~/media/1ea8aec3bfa24543a28fcaea25986514... -
Tips for arabidopsis transformation
TypeBlog Post...Cold Spring Harbor Laboratory Press. Available at: https://books.google.at/books/about/Arabidopsis.html?id... -
New CRISPR Tools: Cas7-11 and PASTE
TypeBlog Post...CRISPR-directed integrases bioRxiv 2021. 11.01.466786; doi: https://doi.org/10.1101/2021.11.01.466786 Additional Resources... -
Hot Plasmids - November 2023
TypeBlog Post...envelope design. Nature Communications, 14(1), 5141. https://doi.org/10.1038/s41467-023-40788-8. PMID 37612276... -
pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs
TypeBlog Post... and stem cells. Connect with her on LinkedIn @ https://www.linkedin.com/in/kmukherjeephd/. References... -
Antibiotic Resistance: An Old Solution but a New Problem
TypeBlog Post...check bacteria for antibiotic resistance. Source: https://www.youtube.com/watch?v=sx1uDYSfINA. When you ... -
Plasmids 101: E. coli Strains for Protein Expression
TypeBlog Post...More Plasmids 101 Articles? Find them all here: http://blog.addgene.org/topic/plasmids-101 ... -
Tips for Using FRET in Your Experiments
TypeBlog Post...Basics and Applications: An EAMNET Teaching Module http://www.embl.de/eamnet/downloads/modules/FRET_teaching_module.pdf... -
Building Global Connections with the International Mentorship Program USA-EUROPE
TypeBlog Post...Director of Strategic Initiatives. Learn more at https://www.linkedin.com/in/lopezdiegothinkbrain ... -
Important Considerations When Using AAVs
TypeBlog Post...Muscle AAV1, AAV6, AAV7, AAV8, AAV9 Adapted from https://www.addgene.org/viral-vectors/aav/aav-guide. Where... -
Sequencing Options for CRISPR Genotyping
TypeBlog Post...a more just and equitable society. Read more at https://magazine.scienceforthepeople.org/. References... -
Chemogenetics Guide
TypeGuide...Neuronal Biology. Physiol Rev, 98 (1), 391–418. https://doi.org/10.1152/physrev.00009.2017 PMID: 29351511...ligand. Proc Natl Acad Sci USA, 104 (12), 5163–5168. https://doi.org/10.1073/pnas.0700293104 PMID: 17360345...activation. Proc Natl Acad Sci USA, 113 (3), E358–E367. https://doi.org/10.1073/pnas.1510899113 PMID: 26733686...bioluminescence. Neurophotonics 11 (2), 021005. https://doi.org/10.1117/1.NPh.11.2.021005 PMID: 38450294...receptor. Proc Natl Acad Sci USA, 95 (1), 352–357. https://doi.org/10.1073/pnas.95.1.352 PMID: 9419379 Davies... actions? Psychopharmacology, 178 (4), 451–460. https://doi.org/10.1007/s00213-004-2017-1 PMID: 15765260...Neurons. Neuropsychopharmacol, 38 (5), 854–862. https://doi.org/10.1038/npp.2012.251 PMID: 23303063 Gomez... -
Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein
TypeBlog Post... favourite gene(s) from Ensembl genome browser: https://www.ensembl.org/index.html Design crRNA/ssODN:... -
With an Eye Towards the Future, We Look Back at the March for Science
TypeBlog Post...same time pass over in silence his very large one” (http://oll.libertyfund.org/titles/galilei-dialogues-concerning-two-new-sciences... -
Lentiviral Vector Guide
TypeGuide...Pseudotyping. Current Gene Therapy , 5 (4), 387–398. https://doi.org/10.2174/1566523054546224 PMID: 16101513...System. Journal of Virology , 72 (11), 8463–8471. https://doi.org/10.1128/jvi.72.11.8463-8471.1998 PMID:...of lentiviral vectors. Viruses , 3 (2), 132–159. https://doi.org/10.3390/v3020132 PMID: 22049307 Ghaleh.... Biomedicine & Pharmacotherapy , 128 , 110276. https://doi.org/10.1016/j.biopha.2020.110276 PMID: 32502836... Methods & Clinical Development , 21 , 451–465. https://doi.org/10.1016/j.omtm.2021.03.018 PMID: 33981779... Methods & Clinical Development , 20 , 792–800. https://doi.org/10.1016/j.omtm.2020.10.012 PMID: 33768124...Integration Targeting. PLoS ONE , 2 (12), e1340. https://doi.org/10.1371/journal.pone.0001340 PMID: 18092005... -
Optogenetics Guide
TypeGuide...chemical-genetic control of neurons. PLoS ONE, 8 (3), e59759. https://doi.org/10.1371/journal.pone.0059759 PMID: 23544095...neural activity. Nat Neurosci, 8 (9), 1263–1268. https://doi.org/10.1038/nn1525 PMID: 16116447 Chuong, ...microbial rhodopsin. Nat Neurosci, 17 (8), 1123–1129. https://doi.org/10.1038/nn.3752 PMID: 24997763 Deisseroth...brain circuits. J Neurosci, 26 (41), 10380–10386. https://doi.org/10.1523/JNEUROSCI.3863-06.2006 PMID: 17035522...Retrospective and a Look Ahead. Adv Genet, 6 (3), e00021. https://doi.org/10.1002/ggn2.202500021 PMID: 41036479 ...biological systems. Nat Rev Methods Primers, 2 , 55. https://doi.org/10.1038/s43586-022-00136-4 PMID: 37933248...extending optogenetics. Cell, 141 (1), 154–165. https://doi.org/10.1016/j.cell.2010.02.037 PMID: 20303157... -
Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2: Reprogramming and Transdifferentiation
TypeBlog Post... Medical School. Connect with her on LinkedIn @ https://www.linkedin.com/in/kmukherjeephd/. References... -
Adenovirus Guide
TypeGuide...strategies . Current Gene Therapy, 11 (4), 307–320. https://doi.org/10.2174/156652311796150372 (Link opens...Signal Transduction and Targeted Therapy, 6 (1), 53. https://doi.org/10.1038/s41392-021-00487-6 (Link opens...virus vaccine . Scientific Reports, 10 (1), 3613. https://doi.org/10.1038/s41598-020-60238-5 (Link opens...Vaccines & Immunotherapeutics, 13 (12), 3020–3032. https://doi.org/10.1080/21645515.2017.1383575 (Link opens...challenge . Journal of Virology, 85 (9), 4222–4233. https://doi.org/10.1128/JVI.02407-10 (Link opens in a ... Expert Review of Vaccines, 14 (10), 1347–1357. https://doi.org/10.1586/14760584.2015.1077122 (Link opens...the United States of America, 95 (5), 2509–2514. https://doi.org/10.1073/pnas.95.5.2509 (Link opens in ...