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We narrowed to 486 results for: mag

Showing: 261 - 280 of 486 results
  1. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    ...would help you do the job as described. Let’s imagine you’re applying to a job that requires ddPCR skills...answer to that is what goes into your cover letter. Imagine you’re applying to a job with a lot of file management...
  2. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    ... appear darker than full vector particles. This image shows that the vast majority of the vectors consist...particles (black arrowhead). Scale bar = 100nm. Image courtesy of David Bell and Svetla Stoilova-McPhie...
  3. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ...groups have utilized dCas13 fusion proteins for imaging, tracking, modulating splicing, and regulating ...fluorescent fusion protein dLwaCas13a-NF for in vivo RNA imaging. In short, the Cas13a molecule brings a much anticipated...
  4. Tips for Getting a Faculty Position

    Type
    Blog Post
    ...schools. I will emphatically deny that I have a magic formula to guarantee a reader the faculty job of...optimization of fluorescent proteins,  and live cell imaging approaches. Erik is also an avid long distance ...
  5. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ...now to capture interesting key results, striking images, creative ideas, and engaging stories as you encounter...supportive audience? 7. Hone Your slides Use big images that are easy to see (and for heavens sakes, label...
  6. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ... is twice the length of the monomer (~11.6 kb). Image generated by SnapGene software.  Thanks to ...multimer identification.   Fig. 2: Gel image of Plasmid 61564: pBAMD1-2 (~4.7 kb) digested with...
  7. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...paper. Pretty much like an actual sandwich! S'mores image from Evan Amos.   After the transfer, some areas...chemiluminescence, is detected on x-ray film or with specific imaging devices. In most cases, the primary antibody, which...
  8. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...5, 6, 8, and 9 in two different brain regions.  Image modified from Aschauer et al., 2013, Figure 1. Used...both oligodendrocytes (Olig2) and neurons (NeuN). Image from Griffin et al., 2019, Figure 4. Used under ...
  9. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...aureus Cas9 (Sa-Cas) and deposited plasmid IF311: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-LSD1. LSD1 is a particularly.... These two vectors are part of the larger pMVP/pMAGIC cloning system, which contains Cas9 fusions for...
  10. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...cellular repair mechanisms rush in to repair the damage. If all they can do is stitch the two broken ends...Mizutani E, Sato H, Kasai M, Ogawa A, Suchy F, Yamaguchi T, Nakauchi H (2018) Intra-embryo Gene Cassette...
  11. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...cargo with the excision-only piggyBac™ transposase. Image courtesy of Transposagen.   How does piggyBac... selection cassette by excision-only piggyBac™. Image courtesy of Transposagen.   How does the piggyBac...
  12. Protocol - How to Purify DNA from an Agarose Gel

    Type
    Protocol
    ...and nearest sample. To minimize the risk of DNA damage, it is best to limit the UV exposure of the DNA...DNA. Therefore, it is a bad idea to use a gel imager to take a picture of the gel before cutting out the...
  13. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ...scientist was like. The word “scientist” conjured up images of men like Albert Einstein and Bill Nye in their...postdocs I’ve met have done things that I never even imagined before I set foot in the lab. They’re also more...
  14. Transfection for Recombinant Antibodies

    Type
    Protocol
    ...set to 120 rpm 37 °C bead bath Vortex Stir bar Magnetic stir plate pH meter Reagents HEK293 cells Recombinant... deionized water in a 1 L bottle and stir on a magnetic stir plate. Stir until all particles have dissolved...
  15. Protocol - How to Run an Agarose Gel

    Type
    Protocol
    ...solution and then rinse it in water before you can image the gel. Pour the agarose into a gel tray with the...expose for as little time as possible to minimize damage to the DNA. Note: When using UV light, protect ...
  16. Deep Dive: qPCR

    Type
    Blog Post
    ...dimers. (c) A double peak indicating 2 amplicons. Image credits (respectively): Zuzanna K. Filutowska; Selma...allows, you can fit in a quick game of Connect Four! Image created in BioRender.   Passive Reference Dyes...
Showing: 261 - 280 of 486 results