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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 474 results for: mag

Showing: 261 - 280 of 474 results

  1. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...aureus Cas9 (Sa-Cas) and deposited plasmid IF311: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-LSD1. LSD1 is a particularly.... These two vectors are part of the larger pMVP/pMAGIC cloning system, which contains Cas9 fusions for...

  2. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...cellular repair mechanisms rush in to repair the damage. If all they can do is stitch the two broken ends...Mizutani E, Sato H, Kasai M, Ogawa A, Suchy F, Yamaguchi T, Nakauchi H (2018) Intra-embryo Gene Cassette...

  3. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...cargo with the excision-only piggyBac™ transposase. Image courtesy of Transposagen.   How does piggyBac... selection cassette by excision-only piggyBac™. Image courtesy of Transposagen.   How does the piggyBac...

  6. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ...scientist was like. The word “scientist” conjured up images of men like Albert Einstein and Bill Nye in their...postdocs I’ve met have done things that I never even imagined before I set foot in the lab. They’re also more...

  7. Protocol - How to Purify DNA from an Agarose Gel

    Type
    Protocol
    ...and nearest sample. To minimize the risk of DNA damage, it is best to limit the UV exposure of the DNA...DNA. Therefore, it is a bad idea to use a gel imager to take a picture of the gel before cutting out the...

  9. Deep Dive: qPCR

    Type
    Blog Post
    ...dimers. (c) A double peak indicating 2 amplicons. Image credits (respectively): Zuzanna K. Filutowska; Selma...allows, you can fit in a quick game of Connect Four! Image created in BioRender.   Passive Reference Dyes...

  10. Transfection for Recombinant Antibodies

    Type
    Protocol
    ...set to 120 rpm 37 °C bead bath Vortex Stir bar Magnetic stir plate pH meter Reagents HEK293 cells Recombinant... deionized water in a 1 L bottle and stir on a magnetic stir plate. Stir until all particles have dissolved...

  11. Protocol - How to Run an Agarose Gel

    Type
    Protocol
    ...solution and then rinse it in water before you can image the gel. Pour the agarose into a gel tray with the...expose for as little time as possible to minimize damage to the DNA. Note: When using UV light, protect ...

  12. Western Blot

    Type
    Protocol
    ...Device Roller Spatula Platform shaker Cold room Gel imager -80 °C freezer Reagents 1X PBS Lysis buffer e.g...Cover the membrane with clear plastic and use a gel imager with chemiluminescence detection or a dark room...

  13. Kit Free RNA Extraction

    Type
    Protocol
    ...temperature, as prolonged storage in the cold room may damage it. Using a pipettor, transfer the top, aqueous...
Showing: 261 - 280 of 474 results