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We narrowed to 477 results for: mag

Showing: 261 - 280 of 477 results

  1. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...5, 6, 8, and 9 in two different brain regions.  Image modified from Aschauer et al., 2013, Figure 1. Used...both oligodendrocytes (Olig2) and neurons (NeuN). Image from Griffin et al., 2019, Figure 4. Used under ...

  3. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...cellular repair mechanisms rush in to repair the damage. If all they can do is stitch the two broken ends...Mizutani E, Sato H, Kasai M, Ogawa A, Suchy F, Yamaguchi T, Nakauchi H (2018) Intra-embryo Gene Cassette...

  4. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...cargo with the excision-only piggyBac™ transposase. Image courtesy of Transposagen.   How does piggyBac... selection cassette by excision-only piggyBac™. Image courtesy of Transposagen.   How does the piggyBac...

  5. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    ... is twice the length of the monomer (~11.6 kb). Image generated by SnapGene software.  Thanks to ...multimer identification.   Fig. 2: Gel image of Plasmid 61564: pBAMD1-2 (~4.7 kb) digested with...

  6. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    ...scientist was like. The word “scientist” conjured up images of men like Albert Einstein and Bill Nye in their...postdocs I’ve met have done things that I never even imagined before I set foot in the lab. They’re also more...

  9. Protocol - How to Purify DNA from an Agarose Gel

    Type
    Protocol
    ...and nearest sample. To minimize the risk of DNA damage, it is best to limit the UV exposure of the DNA...DNA. Therefore, it is a bad idea to use a gel imager to take a picture of the gel before cutting out the...

  11. Transfection for Recombinant Antibodies

    Type
    Protocol
    ...set to 120 rpm 37 °C bead bath Vortex Stir bar Magnetic stir plate pH meter Reagents HEK293 cells Recombinant... deionized water in a 1 L bottle and stir on a magnetic stir plate. Stir until all particles have dissolved...

  12. Deep Dive: qPCR

    Type
    Blog Post
    ...dimers. (c) A double peak indicating 2 amplicons. Image credits (respectively): Zuzanna K. Filutowska; Selma...allows, you can fit in a quick game of Connect Four! Image created in BioRender.   Passive Reference Dyes...

  13. Protocol - How to Run an Agarose Gel

    Type
    Protocol
    ...solution and then rinse it in water before you can image the gel. Pour the agarose into a gel tray with the...expose for as little time as possible to minimize damage to the DNA. Note: When using UV light, protect ...

  14. Western Blot

    Type
    Protocol
    ...Device Roller Spatula Platform shaker Cold room Gel imager -80 °C freezer Reagents 1X PBS Lysis buffer e.g...Cover the membrane with clear plastic and use a gel imager with chemiluminescence detection or a dark room...

  15. Plan Your Experiment

    Type
    Guide
    ...systems utilized for CRISPR/Cas gene editing. This image summarizes ways that the CRISPR/Cas system can be...

  16. Kit Free RNA Extraction

    Type
    Protocol
    ...temperature, as prolonged storage in the cold room may damage it. Using a pipettor, transfer the top, aqueous...

  17. Adenovirus Guide

    Type
    Guide
    ...that responds to general signals of infection or damage. Insertional mutagenesis Random insertion of DNA...
Showing: 261 - 280 of 477 results