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Showing: 261 - 280 of 961 results
  1. Using Video to Share Your Science: We Share Science

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    Blog Post
    ...Watkins, Professor at George Washington University and developer of wesharescience.org. Sharing your research...professor at George Washington University and he the developer of the WeShareScience.org platform. You...study processes for diagnosing and resolving organizational performance and often have to be able to quickly...sharing video abstracts about research: www.WeShareScience.org. WeShareScience is a free platform for ...Sharing in Research Publications Resources at Addgene.org Check out our Protocol Pages for Video Protocols...
  2. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

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    Blog Post
    ...without DNA cleavage. Nature 551:464–471 . https://doi.org/10.1038/nature24644 Glaser A, McColl B, Vadolas...Molecular Therapy - Nucleic Acids 5:e334 . https://doi.org/10.1038/mtna.2016.48 Katti A, Foronda M, Zimmerman... Nucleic Acids Research 48:2841–2852 . https://doi.org/10.1093/nar/gkaa124 Martin AS, Salamango DJ, Serebrenik...editosome complexes. Scientific Reports 9: . https://doi.org/10.1038/s41598-018-36739-9 St. Martin A, Salamango...cells. Nucleic Acids Research 46:e84–e84 . https://doi.org/10.1093/nar/gky332 Standage-Beier K, Tekel SJ, ... Nucleic Acids Research 47:e120–e120 . https://doi.org/10.1093/nar/gkz713 Additional resources on the ...reporters Browse our CRISPR blog posts Resources on Addgene.org Browse the CRISPR guide Find CRISPR plasmids...
  3. Hot Plasmids: Fall 2024

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    Blog Post
    ...Anti-V5 [IPI-SV5-Pk1]. Addgene Report, https://doi.org/10.57733/addgene.l0ulu4. Hanke, T., Szawlowski,...antigen. J Gen Virol., 73 (Pt 3), 653–660. https://doi.org/10.1099/0022-1317-73-3-653. PMID 1372038. Randall.... J Gen Virol., 68 (Pt 11), 2769–2780. https://doi.org/10.1099/0022-1317-68-11-2769. PMID 2445904.   ...protein degraders. Nat Commun., 15(1), 8885. https://doi.org/10.1038/s41467-024-52871-9. PMID 39406745. Optimizing...Caenorhabditis elegans. Genetics, 228(2), iyae126. https://doi.org/10.1093/genetics/iyae126.   Gene disruption in...abscessus. J Bacteriol., 206(3), e0033523. https://doi.org/10.1128/jb.00335-23.  Bioluminescent reporting...Systems. ACS Sensors, 9(7), 3489–3495. https://doi.org/10.1021/acssensors.3c02664. ...
  4. Tips for Writing a Good Cover Letter

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    Blog Post
    ...asks for strong organizational skills. You might think, “I’d be good at that! I organized my lab data so...important part of the application. Even within an organization, there can be variance. At Addgene, our content...explain why you are interested in working for that organization or group. This is particularly important for...want to talk about! You could describe your organizational system, your rationale for designing it the...title of the position] at [name of the company/organization]. I am a [adjective that describes you] scientist...that particular job, your commitment to the organization's mission, or your deep interest in the lab's...
  5. Antibody Validation for Flow Cytometry

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    Blog Post
    ...efforts and information provided by external organizations like the Human Cell Differentiation Molecules...research applications. eLife, 12, RP91645. https://doi.org/10.7554/eLife.91645 Biddle, M. S., Alende, C., ...flow cytometry. F1000Research, 13, 817. https://doi.org/10.12688/f1000research.154034.1 Laflamme, C., McKeever...disease gene C9ORF72. eLife, 8, e48363. https://doi.org/10.7554/eLife.48363 Uhlen, M., Bandrowski, A., ...antibodies. Nature Methods, 13(10), 823–827. https://doi.org/10.1038/nmeth.3995 Virk, H. S., Biddle, M. S., ...Journal of Pharmacology, 178(15), 2948–2962. https://doi.org/10.1111/bph.15467 Virk, H. S., Rekas, M. Z., Biddle...TRPA1. Scientific Reports, 9(1), 18500. https://doi.org/10.1038/s41598-019-55133-7 More resources from ...
  6. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

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    ...detection and identification using FnCas9. https://doi.org/10.1101/2020.04.07.028167 Broughton JP, Deng X,...detection of SARS-CoV-2. Nature Biotechnology. https://doi.org/10.1038/s41587-020-0513-4 Kellner MJ, Koob JG, ...nucleases. Nature Protocols 14:2986–3012 . https://doi.org/10.1038/s41596-019-0210-2 Lucia, C., Federico, ...-Cas12. Cold Spring Harbor Laboratory. https://doi.org/10.1101/2020.02.29.971127 Rauch, J. N., Valois,...Material. Cold Spring Harbor Laboratory. https://doi.org/10.1101/2020.04.20.052159 Zhang, F., Abudayyeh,...COVID-19 using CRISPR diagnostics. https://www.broadinstitute.org/files/publications/special/COVID-19%20detection...available for COVID-19 research Resources on Addgene.org Get the basics about CRISPR in our CRISPR guide...
  7. Hot Plasmids: Summer 2024

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    Blog Post
    ...samples. bioRxiv 2024.01.21.576499; doi: https://doi.org/10.1101/2024.01.21.576499.    LEA proteins protect...freezing. bioRxiv 2024.02.06.579238. doi: https://doi.org/10.1101/2024.02.06.579238.    Engineered and evolved...Biotechnology, 10.1038/s41587-023-02078-y. doi: https://doi.org/10.1038/s41587-023-02078-y.  Anzalone, A. V., Gao...Nature Biotechnology, 40(5), 731–740. doi: https://doi.org/10.1038/s41587-021-01133-w Pandey, S., Gao, X. ...Engineering, 10.1038/s41551-024-01227-1. doi:  https://doi.org/10.1038/s41551-024-01227-1   CHARM: A compact ...editor. Science, 384(6703), ado7082. doi: https://doi.org/10.1126/science.ado7082.  Nuñez, J. K., Chen, J...editing. Cell, 184(9), 2503–2519.e17. doi: https://doi.org/10.1016/j.cell.2021.03.025.    Recombinant antibodies...
  8. Our Most Popular Blog Posts from 2019

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    Blog Post
    ...Popular Model Organisms by Alyssa Cecchetelli and Lukas Morgan There are many model organisms people are...interview including defining your research vision, organizing your vision into a chalk talk, and tips for practicing.... In this blog post, we’ve covered five model organisms: mouse, fruit fly, yeast, zebrafish, and worm.... by leaving a comment or emailing us at blog@addgene.org.  Thank you again for a fantastic year and happy...
  9. Science as Art

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    Blog Post
    ...to me… The problem is that scientists sometimes forget how to communicate about their work with non-academic... is essential to stay in touch with the living organism I study, to be able to see it all scales. Most... a way to better understand and appreciate the organism, for both scientist and non-scientist alike. “...inner structures of delicate plants and other organisms without destroying the original, which could provide...Do you look for the beauty in the microscopic organisms on your lab bench? Let us know in the comments...Experimenting in an Open Source Lab Resources on Addgene.org Try the Plasmid Land Game ...
  10. Antibodies 101: The Basics of Western Blotting

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    Blog Post
    ...analyses using ELISA. Peptides 72:4–15 . https://doi.org/10.1016/j.peptides.2015.04.012 Burnette WN (1981... Analytical Biochemistry 112:195–203 . https://doi.org/10.1016/0003-2697(81)90281-5 Pillai-Kastoori L,...for the user. J Biol Chem 295:926–939 . https://doi.org/10.1074/jbc.ra119.010472 Renart J, Reiser J, Stark...National Academy of Sciences 76:3116–3120 . https://doi.org/10.1073/pnas.76.7.3116 Towbin H, Staehelin T, Gordon...National Academy of Sciences 76:4350–4354 . https://doi.org/10.1073/pnas.76.9.4350 Yang P-C, Mahmood T (2012...trouble shooting. North Am J Med Sci 4:429 . https://doi.org/10.4103/1947-2714.100998 Additional resources on...articles related to antibodies Resources on Addgene.org Find written and video protocols Visit Addgene's...
  11. Exploring Applications of the Bioluminescent HiBiT Tag

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    Blog Post
    ...ACS Chemical Biology, 7(11), 1848–1857. https://doi.org/10.1021/cb3002478 Kamada, Y., Tateishi, H., Nakayamada...of Misfolded CFTR. Cells, 12(23), 2741. https://doi.org/10.3390/cells12232741  Kamio, T., Kono, Y., Hirosuna...Gastric Cancer. Cancer Medicine, 14(8). https://doi.org/10.1002/cam4.70793 Laissue, P. P., Alghamdi, R....imaging. Nature Methods, 14(7), 657–661. https://doi.org/10.1038/nmeth.4344  Peach, C. J., Kilpatrick, L...Journal of Pharmacology, 178(12), 2393–2411. https://doi.org/10.1111/bph.15426 Promega Corporation (2025). Illuminating...and in vitro. Oncology Reports, 50(5). https://doi.org/10.3892/or.2023.8631 Tsien R. Y. (1998). The green...Annual Review of Biochemistry, 67, 509–544. https://doi.org/10.1146/annurev.biochem.67.1.509  More resources...
  12. The Golden Stopwatch Award: Recognizing Facilitators of Research

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    Blog Post
    ...here yet? A quick look at your order status on addgene.org identifies the hold-up. Your Material Transfer...possible Tell them about the Golden Stopwatch! Any organization that deposits with and/or requests materials...to Addgene’s Tech Transfer Team: techtransfer@addgene.org Congratulations to the Golden Stopwatch awardees...to all of the winners so far! Don’t see your organization below? Don’t worry--we check periodically for...transfer offices. Drop us a line at techtransfer@addgene.org. Albert Einstein College of Medicine  Baylor...of commercialization Additional resources on Addgene.org Addgene Help Center: Tech Transfer Technology...
  13. CRISPR 101: Non-Homologous End Joining

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    Blog Post
    ...Mutagenic Potential. PLoS Genet 5:e1000683 . https://doi.org/10.1371/journal.pgen.1000683 Bétermier M, Bertrand...Error-Prone Process? PLoS Genet 10:e1004086 . https://doi.org/10.1371/journal.pgen.1004086 Ghezraoui H, Piganeau...End-Joining. Molecular Cell 55:829–842 . https://doi.org/10.1016/j.molcel.2014.08.002 Ran FA, Hsu PD, Lin...Editing Specificity. Cell 154:1380–1389 . https://doi.org/10.1016/j.cell.2013.08.021 Waters CA, Strande NT...nonhomologous end joining. Nat Commun 5: . https://doi.org/10.1038/ncomms5286 Yan M-Y, Li S-S, Ding X-Y, Guo...Mycobacterium tuberculosis. mBio 11: . https://doi.org/10.1128/mbio.02364-19 More CRISPR Blog Posts from...
  14. Plasmids for Endogenous Gene Tagging in Human Cells

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    Blog Post
    ...Cell Explorer at allencell.org and the Allen Institute website at alleninstitute.org. A classic challenge...Cell Explorer at allencell.org and the Allen Institute website at alleninstitute.org. References 1. Roberts.../Cas9 in human stem cells to illuminate cell organization." bioRxiv (2017): 123042. Additional Resources... Our Fluorescent Protein Posts Resources on Addgene.org Browse CRISPR Tagging Plasmids Browse the Fluorescent...
  15. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

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    Blog Post
    ...modulating SARS-CoV-2 infection. bioRxiv. https://doi.org/10.1101/2021.04.22.440848 Crawford KHD, Eguia R...Neutralization Assays. Viruses 12:513. https://doi.org/10.3390/v12050513 Hsieh C-L, Goldsmith JA, Schaub...SARS-CoV-2 spikes. Science 369:1501–1505. https://doi.org/10.1126/science.abd0826 Tong P, Gautam A, Windsor... of evolving SARS-CoV-2 spike bioRxiv. https://doi.org/10.1101/2021.03.10.434840 Tsang J, LaManna CM (...Detection Tools. The CRISPR Journal 3:142–145. https://doi.org/10.1089/crispr.2020.0030 Additional resources on...Browse all COVID-19 blog posts Resources on Addgene.org Find COVID-19 resources on our COVID-19 and ...
  16. Design Tips for Prime Editing

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    Blog Post
    ...donor DNA. Nature, 576(7785), 149–157. https://doi.org/10.1038/s41586-019-1711-4 Chen, P. J., Hussmann...outcomes. Cell, 184(22), 5635-5652.e29. https://doi.org/10.1016/j.cell.2021.09.018 Nelson, J. W., Randolph... Nature Biotechnology, 40(3), 402–410. https://doi.org/10.1038/s41587-021-01039-7 Nishimasu, H., Ran, ...and Target DNA. Cell, 156(5), 935–949. https://doi.org/10.1016/j.cell.2014.02.001 Yan, J., Oyler-Castrillo...RNA-binding protein. Nature, 628(8008), 639–647. https://doi.org/10.1038/s41586-024-07259-6 Additional Resources... Download the CRISPR 101 eBook Resources on Addgene.org Read the CRISPR guide Find CRISPR plasmids by...
  17. Experimenting in an Open Source lab: from CRISPR to Cats

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    Blog Post
    ... https://wiki.hackerspaces.org/Hackerspaces OpenWorm Project Website: http://www.openworm.org/ Cat Microbiome...1,000 active makerspaces around the world. Hackerspaces.org maintains a list of active spaces near you...While C. elegans are often thought of as simple organisms , they have many levels of complexity, especially...collection of samples and sequencing is being organized and funded by someone else (KittyBiome has been...University at NASA Ames, and an instructor at Biocurious.org, Derek is bringing cutting edge technologies...
  18. How-to: 5 Steps to a Great Panel Discussion

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    Blog Post
    ...result of the content. Here are some of my tips to organizing and running a memorable panel discussion. Check...make it look effortless to the audience, but the organizers and moderator should have a well-planned approach...panelists to the moderator. The moderator and organizers should develop a list of 4-6 pre-event interview...interview questions. The moderator (possibly with an organizer) should set up a 30 minute call with each panelist...example, at a recent event on nonprofit science organizations, I asked audience members to stand and describe...networking time. Both the moderator and program organizers can help make sure the event stays on schedule...
  19. How to Deposit Your Plasmids with Addgene

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    Blog Post
    ...e-mail us at  deposit@addgene.org Special cases Please contact us at deposit@addgene.org or +1 (617)-ADDGENE...plasmids, please let us know by contacting deposit@addgene.org. These deposits are considered large deposits.... Email the completed spreadsheet to deposit@addgene.org along with your GenBank files, plasmid sequences...library with Addgene?" or contact us at deposit@addgene.org or +1 (617)-ADDGENE (233-4363) with additional...to your strain. Please contact us at deposit@addgene.org or +1 (617)-ADDGENE (233-4363) if you’d like ...after submission, please email us at deposit@addgene.org. Include your deposit number and which data needs...
  20. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

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    Blog Post
    ... spatial genome organization deciphering. Molecular Cytogenetics, 11. https://doi.org/10.1186/s13039-018... 2. Determining the spatial and hierarchical organization of chromatin interaction networks  A second ...Biotinylated dCas9. Cell 170:1028–1043.e19 . https://doi.org/10.1016/j.cell.2017.08.003 Liu, X., Chen, Y., Zhang...biotinylated dCas9. Genome Biology, 21. https://doi.org/10.1186/s13059-020-01973-w Additional Resources... Favorite CREs Here Additional Resources on Addgene.org Find Other Cas9 Variants for Purifying Genomic...
Showing: 261 - 280 of 961 results