We narrowed to 303 results for: rigi

1. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

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   ...maintained in dividing cells because they lack an origin of replication (Banasik and McCray, 2010). However...

2. Hot Plasmids: Summer 2024

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   ... each other, while the 5’ flaps containing the original DNA sequence anneal with each other. Excision ...

3. Antibodies 101: Beyond Surface Labeling

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   ...blotting, a primary epitope-targeting antibody can originate from a range of species; for example, rabbit or...

4. Antibodies 101: Introduction to Gating in Flow Cytometry

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   ...expected size. The strong signals close to the graph’s origin mainly stem from cell debris and other small fragments...

5. Fluorescent Proteins 101: When GFP lets you down

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   ... introducing two mutations (F64L, S65T) in the original jellyfish GFP (AvGFP) to create enhanced GFP (...

6. New Optogenetic Tools for Cytoskeleton and Membrane Control

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   ... 10, 30, 50, and 100 times the activity of the original, generated using directed evolution (Tei et al...

7. Tips for Getting a Faculty Position

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   ... at [email protected]." You can find his original blog post below! --Ed.  Eight years ago, I decided...

8. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

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   ...did not affect the function or efficiency of the original APEX (Lam et al. 2014). They named this mutant...

9. Harnessing Bacterial Toxins for Allelic Exchange

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   ...chloramphenicol resistance cassette, and the R6K origin of replication to allow replication only in hosts...

10. Five Popular Model Organisms

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    ...actually two distinct populations of β-cells that originate in different regions of the pancreas and produce...

11. Quick Guide to Near-Infrared Fluorescent Proteins

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    ...normalization to fluorescence of co-transfected EGFP. e Originally reported as a monomer16, IFP2.0 was later found...

12. CRISPR 101: Validating Your Genome Edit

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    ...This post was originally written by Melina Fan and updated Nov 3, 2022 by Susanna Stroik. You’ve designed...

13. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

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    ...Originally published Oct 11, 2016 and last updated Dec 22, 2020 by Benoit Giquel. CRISPR has taken the...

14. Viral Vectors 101: AAV Serotypes and Tissue Tropism

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    ...for contributing their expertise! This post was originally published in January 2025 and updated in August...

15. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

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    ... a standard dsDNA repair template is used. The original design for creating a conditional allele, or floxed...

16. CRISPR 101: Epigenetics and Editing the Epigenome

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    ...Originally published Feb 14, 2017 and updated Jun 24, 2020. Epigenetic modifications are an additional...

17. Recombinase-based State Machines Enable Order-dependent Logic in vivo

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    ...Input Plasmid: The input plasmid (high copy, ColE1 origin of replication), contains large serine recombinase...

18. PiggyBac-ing Through the Genome Editing Field

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    ...piggyBac™ transposon? The piggyBac™ transposon was originally identified in moths in 1983, but wasn’t successfully...

19. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

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    ... red genes from a plasmid with a heat sensitive origin of replication.  Once the recombineering system...

20. When Fidelity Matters: A frank discussion about ligase fidelity

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    ...can reliably detect single-base differences. The original creators of this technique visualized the ligation...