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We narrowed to 602 results for: she

Showing: 261 - 280 of 602 results

  1. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ...(Gootenberg et al., 2017). This system, dubbed SHERLOCK (depicted in figure 1) has been used to differentiate...applications of CRISPR. Since this post was originally published, we've also seen the new development of Cas13b...al., ‘Point-of-care testing for COVID-19 using SHERLOCK diagnostics.’ medRxiv 2020 May 8. Pubmed PMID:...

  2. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ... Originally published Jul 14, 2015 and last updated Sep 16, 2020 by Beth Kenkel.  CRISPR genome editing...Cas12b and CasX. While there are no papers yet published that deliver these Cas proteins with AAV, at just...0908-x  Nishimasu H, Ran FA, Hsu PD, Konermann S, Shehata SI, Dohmae N, Ishitani R, Zhang F, Nureki O (2014...

  3. The Challenges of Cell Culture

    Type
    Blog Post
    ... incubator). This means any other scientist who wishes to use these cells can see their passage number...immortal life of Henrietta Lacks. New York: Crown Publishers, 2010. 2. Gold, Michael. "A conspiracy of cells...Additional Resources ATCC Animal Cell Culture Guide Thermofisher Guidelines for Maintaining Cultured Cells Invitrogen...

  4. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    ... is a loop in between secondary structure (beta-sheets or alpha helices). An additional requirement is...be generated with a transposon-based strategy (Sheridan, 2002). Avoiding protein over-expression artifacts...PMID: 18454154. PubMed Central PMCID: PMC2853173. Sheridan, Douglas L., et al. "A new way to rapidly create...

  5. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...depending on the structure of the TO derivative (Dolgosheina et al., 2014). While 10 derivatives of TO exist...ingredients in the aptamer soup In addition to these established RNA aptamer fluorophores, more are in development...29440634. PubMed Central  PMCID: PMC5811451. Dolgosheina, Elena V., et al. "RNA mango aptamer-fluorophore...

  6. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    ...repressed more   LacZ β-galactosidase activity abolished   lacY Lactose permease inactivated, lactose...plasmid stability recBCD Exonuclease V activity abolished Increased plasmid stability, reduced recombination...r-m+)  MG1655    Wild type K-12 strain; first published sequence of an E. coli K-12 strain. F- λ- ilvG...

  7. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    ...teams: Watson, Crick, & Franklin, Meselson-Stahl, Hershey-Chase...Who was I to dispute history? And boy did...organization on the planet, in which case whoa! Mission accomplished! One example of a mission-based team in academia...myth. The original Dr. Frankenstein from Mary Shelley’s novel worked alone, but even the movie makers ...

  8. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...Lumio Green and Lumio Red from Invitrogen (now ThermoFisher Scientific). These reagents are nonfluorescent...is that binding is covalent, so free dye can be washed away. In addition, labeling contrast can be improved... developed, so don’t be afraid to try novel, unpublished combinations of labeling strategies. FRET experimental...

  9. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ...around the same time. (The papers were actually published in the same journal on the same day.) This team...lot of terminology in this post! Here’s a quick refresher if you ever lose track of which acronym is which...10.1038/s41556-023-01342-2 Koblan, L. W., Arbab, M., Shen, M. W., Hussmann, J. A., Anzalone, A. V., Doman,...

  10. Western Blot

    Type
    Protocol
    ... buffer Microcentrifuge tubes BCA Assay, Thermo Fisher 23227 β-mercaptoethanol 4X protein loading buffer... ladder Ethanol iBlot 2 PVDF Mini Stack, Thermo Fisher IB24002 20X TBS Tween-20 Nonfat milk powder 96-...deionized water 3x . Shake the gel gently between washes to remove residual water. Load the gel into the...

  11. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    ...CRISPR editing world. The approach, originally published in Nature, is a “search-and-replace” genome editing...Yang, W., Leith, A., Daza, R. M., Martin, B., & Shendure, J. (2022). Precise genomic deletions using paired...s41587-021-00868-w Nelson, J. W., Randolph, P. B., Shen, S. P., Everette, K. A., Chen, P. J., Anzalone, ...

  12. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...optimized primers that may work better than the published ones, so if/when you use HercII or Kapa Hifi the...all FAQs from Le Cong on June 24, 2025 by Alyssa Shepard.   References and Resources References Beumer...Additional resources on the Addgene blog CRISPR Cheat Sheet Build Your CRISPR Vocabulary CRISPR 101: Off-Target...

  13. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...transcription start site. In STARR-seq, randomly sheared genomic libraries are cloned downstream of a minimal...simple and complex DNA constructs Have you ever wished you could design a gene knockdown or overexpression...characterize positive hits. This work has been published in Cell. The Moffat laboratory maintains a website...Center has kindly constructed and deposited an unpublished set of Zika gene plasmids to Addgene’s repository...

  14. CRISPR Guide Update

    Type
    Blog Post
    ...to Cas9. All of this is accompanied by new or refreshed figures made using BioRender.com. Figure... Alyssa Shepard...

  16. CRISPR Guide

    Type
    Guide
    ...platform, termed SHERLOCK, can identify target sequences using a fluorescent reporter. In SHERLOCK, a quenched... the presence of that specific target. SHERLOCK (and SHERLOCKv2) allows for greater amplification and ...bacterial cells and does not occur in mammalian cells. SHERLOCK , developed by the Feng Zhang lab , is a Cas13a-based...flow strips. Other Cas enzymes can be added to SHERLOCKv2 to detect multiple targets. For example, Cas12...Nishimasu, H., Ran, F. A., Hsu, P. D., Konermann, S., Shehata, S. I., Dohmae, N., Ishitani, R., Zhang, F., & ...system. Scientific Reports , 4 (1). PMID: 24954249 Shechner, D. M., Hacisuleyman, E., Younger, S. T., & Rinn...

  17. New Viral Vectors - March 2024

    Type
    Blog Post
    ... serotype pAAV-hDlx-Flex-dTomato-Fishell_7 AAV2 Controls Fishell New serotype pAAV-mDlx-NLS-mRuby2...

  19. Fluorescent Proteins 101: History of Fluorescent Proteins

    Type
    Blog Post
    ...In the late 1980s, another researcher, Douglas Prasher, got the idea that this new green glowing protein...reported its sequence. Soon after that, in 1994, Prasher’s collaborator Martin Chalfie expressed GFP in exogenous...

  20. Bringing Sustainable Practices to the Lab: Innovations

    Type
    Blog Post
    ...labels! Reusing plastic Historically, scientists washed and reused materials such as glass pipettes or ...some of these plastics are high grade and can be washed and reused. This might sound risky in terms of ...
Showing: 261 - 280 of 602 results