Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 799 results for: tec

Showing: 261 - 280 of 799 results

  1. Cultivating Community Science at BosLab

    Type
    Blog Post
    ...Biotechnology is no longer just for those working in academia or industry. At BosLab, we identify ourselves...towards enabling everyone to participate in the biotech revolution. Our lab exists in what appears to be...Because we’re conveniently located next to a major Biotech hub, building up our lab was easy. Most of our ...engineer yeast to glow, produce synthetic scents, detect compounds in the environment, and openly publish...Kaczmarczyk is a co-organizer at BosLab, a community biotech laboratory in Somerville MA. Follow her on twitter...

  2. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ...experimented with adding different proteins to the CGBE architecture. They conducted a CRISPRi screen to identify...forms a chemical crosslink with DNA abasic sites, protecting them from cellular processes that would cause...target-library analysis, and machine learning. Nature Biotechnology, 39(11), 1414–1425. https://doi.org/10.1038/...optimization and ancestral reconstruction. Nature Biotechnology, 36(9), 843–846. https://doi.org/10.1038/nbt...targeted DNA transversions in human cells. Nature Biotechnology, 39(1), 41–46. https://doi.org/10.1038/s41587...enable C-to-A and C-to-G base changes. Nature Biotechnology, 39(1), 35–40. https://doi.org/10.1038/s41587...

  3. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    ...it’s true that adding histones and chromatin architecture to every diagram portraying some aspect of eukaryotic...when I recently attended the 2018 Chromosome Architecture and Chromosome Organization Keystone Symposium...posters I saw at the 2018 Keystone Chromosome Architecture and Chromosome Organization. These aren’t the...Crabtree GR Cell. 1998 Nov 25. 95(5):625-36. An architectural role for a nuclear noncoding RNA: NEAT1 RNA ...epigenome. If you have been considering some new techniques, the posts below may steer you in the right direction...

  4. Hot Plasmids - February 2022

    Type
    Blog Post
    ... cellular targets Optimized genetic screening technique using Cas12a for multiplexed gene perturbation...s41467-019-10846-1.   Optimized genetic screening technique using Cas12a for multiplexed gene perturbation... To validate this optimized genetic screening technique, researchers identified a synthetic lethal interaction...future genetic screens.   DeWeirdt PC, et al. Nat Biotechnol. 2021. doi: 10.1038/s41587-020-0600-6. The Penn...tags, are optimized for SDS-PAGE gels and can be detected on Western blots via engineered immunoglobulin...

  5. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    ...quite impressed by how neuroscientists are using techniques from across different disciplines to better understand...many opportunities to learn about and develop technologies that will have impacts on many different facets...ready to discuss their VR helmets, gait tracking technologies, and brain monitors. Walking around the poster... addition to the researcher using sequencing technologies to study microbiome interactions with Alzheimer's... or maybe your favorite FRET-based biosensing technique can be applied to an important neuronal signalling...

  6. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    ...and success. Customer and technical support Our customer and technical service brands continue to ... We score ~99.9% helpful and the customer and technical service teams put 100% effort into helping our...also lauded by another set of stakeholders - the tech transfer community. In a recent law review article...our image in the world. Addgene is now as much a tech company as it is a reagent company. I used to have...

  7. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    ...developing assays.  Protocol architecture The basic (very basic!) architecture of the indirect approach is...direct approach isn’t giving you enough signal to detect your protein of interest, it may be time to look...occasion, usually after other signal amplification techniques have been ruled out.  Ease of access Signal amplification...course, this is much easier said than done! Each technique, and sometimes each individual assay, will need...

  8. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

    Type
    Blog Post
    ...K855A) and eSpCas9(1.1), followed by a technique for detecting genome-wide double strand breaks (BLESS...it’s obvious that CRISPR is a ground-breaking technology that’s already had a huge impact on research ...effects on various biological disciplines, the base technology itself is constantly improving. Cas9 variants... "High-fidelity CRISPR–Cas9 nucleases with no detectable genome-wide off-target effects." Nature (2016...

  9. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...recombination. What is Cre-lox? The Cre-lox system is a technology that can be used to induce site-specific recombination... lethality. To facilitate the use of Cre-lox technology, transgenic mice have been constructed that express...system is another site-directed recombination technology very conceptually similar to Cre-lox, with flippase...analogous to Cre and loxP, respectively. The FLP-FRT technology can be an effective alternative to Cre-lox, and...have any tips or tricks, or clever cre-dependent techniques?  Let us know in the comments! References 1....

  10. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

    Type
    Blog Post
    ...President's Council of Advisors on Science and Technology [PCAST] (2012). Engage to Excel: Producing One...Additional College Graduates with Degrees in Science, Technology, Engineering and Mathematics, Washington, DC:...DC: US Government Office of Science and Technology. 3. Duina, A. A., Miller, M.E., & Keeney, J.B. Budding...BioBuilder Use Addgene Videos to Learn about Basic Lab Techniques High School Students Explore Careers at Addgene...Molecular Biology Reference Pages Learn Simple Lab Techniques in Our Protocol Videos ...

  11. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    ...on to find out more about this amazing imaging technique and find further tips for using FRET in your experiments...proteins is not optimal, FRET may not occur or be detected even if the two proteins form a complex. To help...a potential source of error in suboptimal or undetectable FRET, well-characterized FRET reference standards...environment which alters the FRET efficiency. Detecting certain cellular changes with an intermolecular...the concentration or activity of small molecules, detect cellular processes and signaling cascades, quantify...

  12. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    ...cell stage using one of several techniques. The longest standing technique is the injection of a targeting...before working with mouse models, several common techniques used to create engineered mouse models at embryonic...the pros and cons of different genome editing techniques. Inbred and outbred strains Mouse strains used...Cre is expressed.  Several variations of this technology can be used to customize the effects of this ...

  13. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ...viral handling safety   Containment and personal protection are key to lab safety and the same principles...department if they are not currently provided. Eye protection or face shields are required whenever there’s...splash or spray risk in BSL-1. In BSL-2 labs, eye protection is required any time you have to work with BSL... cabinet. Many researchers choose to wear eye protection when they are working in a biosafety cabinet,...organization. It’s always okay to add another level of protection! When centrifuging viruses (a necessary step...

  14. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    ...science and technology park, it enjoys preferential governmental policies for a high-tech development ...development zone so many science and technology companies and start-ups choose to locate there. I made time to go...because they contain “hard stop” mutations. New techniques used by the Lowe lab have allowed them to begin... and Sun Yat-sen University presented two new technical developments. Using the Tet-on system, his lab...

  15. Plasmids 101: Protein Expression

    Type
    Blog Post
    ... use various techniques to control protein expression for experimental, biotechnological, and medical ...expression systems Plants provide a cheap and low-tech means of mass expression of recombinant proteins...desired protein. Despite being a rather nascent technology, plant cells have been used to express a wide...expression systems however, are expensive and very technically challenging to use. Alyssa D. Cecchetelli is...

  16. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    ...around the globe. Our first story focuses on a technology that has changed virtually all of biology…CRISPR...repository. Other researchers, eager to try this new technology, were quick to request CRISPR plasmids and, in...favorite observations from this data is that CRISPR technology is consistently showing rapid development and...requested through the years, even as more CRISPR technologies become widely used and available. If we look...all of this work helped scientists use CRISPR technology? We think so — and so do our requestors! CRISPR...

  17. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...making them ideal for single-molecule fluorescence techniques. After generating a series of single- and double-tagged...be imaged using one- or two-photon microscopy to detect action potentials and subthreshold events, including...difficult with GEVIs. Not only was ASAP5 able to detect mEPSPs, it also enabled imaging of their propagation...protein and a NanoLuc® (Nluc) Luciferase-fused detector that specifically binds to active (GTP-bound) ...functional heterotrimers with endogenous Gβγ, enabling detection of activation triggered by endogenous GPCR. Example...

  18. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    ...chromoproteins? One reason may be that chromoproteins protect corals from solar irradiation by reflecting visible...gives scientists the ability for instrument-free detection. Unlike fluorescence or luminescence, which require... fluorometers, or luminometers, chromoprotein detection can be done by the naked eye. This makes cloning...more time consuming and expensive methods. While techniques such as  blue-white screening require X-gal, ... (2000) Fluorescent pigments in corals are photoprotective. Nature 408:850–853 . https://doi.org/10.1038...

  19. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

    Type
    Blog Post
    ... standard EGFP plasmid that used to be sold by Clontech and is in a freezer box in just about every lab...environmental changes that the biosensor is designed to detect resulting in false positives [2, 4]. Solutions ...molecular sizing columns and native gels fail to detect EGFP dimerization. Importantly, all of these assays...assays are performed in vitro. Assays to detect FP dimerization in in vivo cellular contexts, such as ... from nonbioluminescent Anthozoa species. Nat Biotechnol, 1999. 17(10): p. 969-73. PubMed PMID: 10504696...

  20. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...To achieve this, scientists use a multitude of techniques including co-transfection of two or more plasmids...started with them. Why Use multicistronic vectors? Detecting cells that are expressing your gene, especially...straightforward process. Rather than try to directly detect your gene of interest, scientists have instead ...not have to exclusively be used as a means of detection; they are useful almost anytime you want to express...upstream of an IRES fluorescent reporter for easy detection.5  Just released: Addgene's Plasmids 101 eBook...
Showing: 261 - 280 of 799 results