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We narrowed to 323 results for: virus

Showing: 261 - 280 of 323 results

  1. Plan Your Experiment

    Type
    Collection
    ...CRISPR delivery, lentiviral and adeno-associated virus (AAV) vectors are the most common. Using viral vectors...into your viral vector) is smaller than that of lentivirus. Thus, AAVs are generally only compatible with...

  2. Biosensor AAV Preps

    Type
    Collection
    ...vector packaging service, but lets us know what viruses would be helpful to the scientific community. Return...

  3. CRISPR Guide

    Type
    Collection
    ... be efficiently packaged into adeno-associated viruses (AAVs) . Other natural Cas orthologs include Hsp1Cas9...editing method in which Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) is fused to the...H840A nickase and M-MLV RT (Moloney Murine Leukemia Virus Reverse Transcriptase) for "search and replace" ...need to generate lentivirus containing the entire library (Figure 8D). This lentivirus will then be used...delivery to cells that are poorly transduced by lentivirus. Non-mammalian CRISPR libraries are also available...as liquid DNA, or in select cases, as pre-made lentivirus . DNA libraries are shipped at a concentration...need to either co-infect with a Cas9-producing lentivirus or use a Cas9-expressing stable cell line. After...

  5. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    ...generate lentivirus (B). Cas9-expressing cells or wild-type cells are treated with lentivirus containing... Quick Guide to All Things Lentivirus Get Tips on Titering Your Lentivirus Plasmids 101: NGS Quality Control...genome wide-screening experiments commonly use lentivirus to deliver a pooled population of gRNAs to Cas9... concentration that is sufficient to generate lentivirus. Be sure to use next-generation sequencing to...the steps above! Cells are then transduced with lentivirus containing the CRISPR library to generate a heterogeneous...

  6. CRISPR Antimicrobials

    Type
    Blog Post
    ... But what if we could specifically target only virulent or antibiotic resistant bacteria with a weapon...no antibiotic strategy available to target only virulent or antibiotic resistant bacteria, and inappropriate...antimicrobials can target specific sequences in a single virulent bacterial species, or even an antibiotic resistance...Bikard et al. used a phagemid system to target virulent S. aureus, a common hospital-borne infection. ...phagemid treatment were sufficient to wipe out the virulent strain. Bacteria not killed by the first round...phagemid treatment decreased the proportion of virulent S. aureus from 50% to 11% in just 24 hours.   ...they suggest that targeting multiple resistance/virulence factors may further reduce this likelihood. The...

  7. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    ...Mammalian Lentivirus pRDA_512 (RSV-driven; lentiGuide-based) Mammalian Lentivirus pRDA_722...lentiCRISPRv2-based; preferred over 512) Mammalian Lentivirus pRDA_789 (CMV-driven; CROPseq) Mammalian...Organism (Mammalian or drosophila) Delivery type (lentivirus, AAV, transposon, Plasmid) Destination vector...

  14. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ...Jaenisch’s lab in plasmid form or as ready-to-use lentivirus. Figure 2: dCas9-Tet1 demethylates cytosines...with the former also available as ready-to-use lentivirus. Figure 3: dCas9-DNMT3A methylates cytosines...

  15. Plasmids for Stem Cell Research

    Type
    Collection
    ...Delivery Method Species Description Article PI Lentivirus Human Expression of human Oct4, Sox2, Klf4, Myc.... Nat Commun. 2018 Jul 6;9(1):2643. Otonkoski Lentivirus Human Expression of human Sox2, Nanog, Oct4, ...Science. 2007 Dec 21;318(5858):1917-20. Thomson Lentivirus Human Doxycycline-inducible expression of human...Stem Cell. 2008 Sep 11. 3(3):346-53. Jaenisch Lentivirus Human Single polycistronic lentiviral vector .... Stem Cells. 2009 May . 27(5):1042-9. Townes Lentivirus Human Single polycistronic lentiviral vector ...Stem Cells Dev. 2010 Aug;19(8):1221-9. Cibelli Lentivirus Human Single polycistronic, doxycycline-inducible...Pluripotency. Cell. 2015 Jul 16;162(2):412-24. Mikkelsen Lentivirus Human Expression of human Klf4, Oct4, c-Myc, ...

  16. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...science.aaa3380 MuLE vectors for customized lentivirus production Finding a lentiviral vector backbone...the process of creating complex polycistronic lentiviruses. The MuLE toolbox contains a series of building-block...tRNA/aaRS pairs are delivered via a pseudotyped baculovirus system, which is advantageous compared to other...

  17. Ginkgo Bioworks COVID-19 Collection

    Type
    Collection
    ...or sort buttons to find SARS-CoV-2 and common coronvirus plasmids based on: Gene insert (e.g. spike, envelope...Codon-optimized expression (recode) Truncated for making pseudovirus (Ctrunc) ID Plasmid Description Mutations Tags...collection at Addgene. Addgene's COVID-19 and Coronavirus Plasmids & Resources page outlines our entire...

  18. 2021 Deck the Lab Winners!

    Type
    Blog Post
    ...them out: https://t.co/uDEE9PsspD#deckthelab #virussnowflakes #sciart #thankyouscience #scicomm pic.twitter.com...
Showing: 261 - 280 of 323 results