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We narrowed to 545 results for: SEC

Showing: 281 - 300 of 545 results

  1. Coomassie Purity Stain of Recombinant Antibodies

    Type
    Protocol
    ...deionized water. Gently invert to mix. Procedure Section 1: SDS-PAGE Add 5 µL of 4X sample buffer to each...Place the gel in the electrophoresis chamber and secure it. Note: Check the manufacturer’s instructions...and bottom part of the gel where dye is visible. Section 2: Staining the Gel Place the gel in a plastic ...sample would fail Addgene QC and would not be used. Section 3: Image Analysis Use ImageJ (Link opens in a new...

  3. The Twelve Days of Antibodies

    Type
    Blog Post
    ...gave to me…  An Antibodies 101 eBook!  🎶On the second day of Antibody, Addgene gave to me…  A recombinant...

  5. Lentiviral Vector Guide

    Type
    Guide
    ... plasmids. Created with BioRender.com. Second-generation Second-generation lentiviral plasmids greatly...contains env All second-generation lentiviral transfer plasmids must be used with a second-generation packaging... either second- or third-generation packaging plasmids. For a summarized comparison of second- and third-generation... Feature Second-generation Third-generation Transfer Plasmid Can be packaged ONLY by a second-generation...thanks to the design of second- and third-generation lentiviral vectors. Second- and third-generation vectors...vector production by removing the accessory genes. Second-generation plasmids include tat , as the 5’ LTR... plasmid, and this requires Tat for activation. Second-generation plasmids include (Figure 3): Transfer...

  6. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    ...miniature CRISPR-Cas system for mammalian use. On the second day of CRISPR, Addgene gave to me: engineered pegRNAs...

  7. The Twelve Days of CRISPR

    Type
    Blog Post
    ... gave to me: a primer on prime editing.  On the second day of CRISPR, Addgene gave to me: SHERLOCK and...

  9. Addgene at SfN23!

    Type
    Blog Post
    ...us at Addgene's booth, #3422, in the nonprofits section.  The Booth What will you find at Addgene's booth...
Showing: 281 - 300 of 545 results