..., mediates protein trans-splicing, similar to an intron in pre-mRNA splicing. In 2016, Fine et al developed...finding smaller Cas9 orthologs works for some applications, it doesn’t eliminate the need to deliver larger...Brown MT, Deshmukh H, Kemp ML, Bao G (2015) Trans-spliced Cas9 allows cleavage of HBB and CCR5 genes in human...

...hour. Find the SHERLOCK plasmids at Addgene! Applications of the SHERLOCK detection system The Zhang lab...portability. This method can be used for an array of applications including clinical diagnostics (e.g. pathogen...patient samples. Find the DETECTR plasmids here! Applications of DETECTR in diagnostics DETECTR allows for...an array of molecular and clinical diagnostic applications. The company Mammoth Biosciences has been started...

...it down until you have something tangible and applicable to your situation.   Thinking Outside the Box...give people more work. Identify your enemies In Felicia Day’s book Embrace your Weird, Day identifies nine...for your idea. Our product team has implemented a policy of never rejecting an idea simply because it's ...innovative group. Our entire company benefits from their policy! Perfectionism When we reach for perfection, our...

...Institute, Public Domain, National Institutes of Health, Wikipedia user Aimecie~commonswiki, Public Domain...let alone the human cost. Here are just a few publications which have since been retracted due to cell ...profiles are available for all the major cell lines on public databases. Image attributions (from top to bottom...

...have real conversations with other scientists. Click Here to Visit Joanne’s AMA I've heard of Reddit,...Anything” was appealing, but also scary. I do a lot of public speaking where I enjoy communicating face to face...posters (this advice included leaving out the term “implicit bias” which is apparently a troll trigger phrase...recommend taking every opportunity you get to speak publically about your work (you can find my tips for a perfect...

... of a mystical "Force," this quote is equally applicable to many microscopy experiments with fluorescent...How can one ensure that an FP fusion with a non-cytosolic protein functions properly? We suggest starting... non-optimized parental proteins. Our recent publication illustrated the quantitative increase in fluorescent...and Jack Griffith, her research focuses on the replication machinery of Kaposi’s sarcoma-associated herpesvirus...

...resistance gene and the origin of replication are necessary to replicate plasmids and make sure the cells...chicken beta actin promoter, and rabbit beta-globin splice acceptor. TRE General expression mRNA Tetracycline... General expression Promoter of the arabinose metabolic operon Inducible by arabinose and repressed catabolite...

...cell types, and research applications. Labeling neurons with Brainbow The Lichtman group developed the original... Brainbow 3.1 and 3.2 (described in the same publication) are unique because by default, they are not ...level of these XFP is not strong enough for all applications. To resolve this, similar systems have been ...

...information on NIR FPs, their properties and applications. At the end, there is a Table that summarizes...their enhanced versions named emiRFPs in your applications.  There is a whole set of spectrally distinct... about the characteristics, performance, and applications of near-infrared calcium indicators in this ...of Medicine. Her expertise is development and applications of fluorescent proteins, genetically encoded...

...Yizhar, and Karl Deisseroth. "The development and application of optogenetics." Annual review of neuroscience...SSharma, Suveena, and Jinfang Zhu. "Immunologic applications of conditional gene modification technology ...Thompson. "Overview on research and clinical applications of optogenetics." Current protocols in pharmacology... J. Simon, et al. "Silencing neurons: tools, applications, and experimental constraints." Neuron 95.3 ...

...resource for anyone looking to use antibody-based applications in their research.  Like all our eBooks, the...through the process. If you’re confident in your application and protocols, but can’t quite seem to find an...for Your Experiment.” Whatever your antibody application question, the Antibody 101 eBook is a great place...

...cellular impediments. FRET has been used in many applications, including drug discovery by big pharma (i.e...innovation, valuable commodities in any NIH grant application! Using FRET, you may gain a new structural understanding...experiments. Donor Acceptor Tag R0 (Å)a Range (Å)b Applications CFP   Lumio Green Tc: CCPGCC 50 40-72 Intact...discovery–a review of theoretical aspects and recent applications." Current Chemical Genomics and Translational...

... required for a growing organism to make the complicated transition from a clump of cells to a fully differentiated...track where GFP labelled B cells were in mounted slices of these organs. These GFP labelled cells could...been created to fate map Langerhans cells and follicular dendritic cells within the skin and lymph node...of antiviral CD8+ T cells in the peripheral interfollicular region of lymph nodes." Nature immunology 9.2...

...up. Applications of photoactivatable fluorescent proteins Some of the most important applications for ...level of background fluorescence. For any PA-FP application, the brighter the fluorescence the better. More...photostable green and red states. Beyond these imaging applications PA-FPs have been developed for use in yeast,...

...sample. Therefore, when a large number of optical slices are acquired all fluorophores throughout the sample...manufacturers have similar information References 1. Lichtman JW, Conchello JA. Fluorescence microscopy. Nature...2005). PubMed PMID: 16299476. 2. Conchello JA, Lichtman JW. Optical sectioning microscopy. Nature methods...2005). PubMed PMID: 16299478. 9. Richardson DS, Lichtman JW. Clarifying Tissue Clearing. Cell 162, 246-...

... approximately three weeks faster, as no license, or license fee, will be required. If you have any questions...Callaway   For researchers in countries with applicable export control exceptions for rabies and VSV-...

...specific subjects in the interview. Happy Listening! Click here to listen! Podcast breakdown: (Time : Topic... PMCID: PMC4096992. Find plasmids from this publication at Addgene. 2. Tang, Jonathan C.Y., et al. “...PubMed PMID: 26258682. Find plasmids from this publication at Addgene. Resources on the Addgene Blog Read...

...Dr. Bengyella, this class of tumors is “a major public health problem, and a leading cause of hospitalization...concluded, Addgene and Seeding Labs will be opening applications for a second round of plasmid awards on June...excited to discover what new and creative ideas applicants will propose for plasmids from the Addgene community...

...expected when we originally sent out the call for applications. Cervical cancer screening in Ghana Gladys is...Papillomavirus (HPV). As Gladys wrote in her application, “Cervical Cancer is the leading cause of cancer...Seeding Labs Plasmid Grant to a third round of applicants at the start of 2017 and would like to thank ...

... that scientific progress made through years of public investment isn't lost. Universities and research...be listed as direct costs from a CRO in grant applications, addressing the new funding structures and indirect... expanding our collaborations with industry and public and private partners to create additional pathways...

... upstream of the sequence of interest. 5. Use a silica spin column for purification of the samples you...Acetate/isopropanol precipitation method or using a silica spin column available from several vendors. The...image below), so I would strongly recommend using a silica spin column. They can be pricey, but well worth...

... about intellectual property rights, pending publications, and commercial use often come up. An MTA can...trials Acknowledgement in recipient’s future publications relating to the material Limited liability...created by the Recipient Right to file patent applications on modifications or inventions Right to publish...

...effectors (TALEs) as anchors for genome engineering applications was still very new. Now, Konermann and her colleagues...In an accompanying Nature News & Views Andreas Möglich and Peter Hegemann described several advantages...disease states. While LITE plasmids have clear application to anyone with an interest in neuroscience, their...

...to encourage constructive input from your team: Solicit feedback regularly and often A one-time event ...ask direct questions for specific feedback in non-public settings. Ask over coffee.  Have regular 1:1 meetings...information to make change whenever possible. Be public about changes influenced by feedback. If you don't...

...with the gRNA), application, cas9 species, PubMed ID, and depositor. The CRISPR applications we currently...and final experimental outcome in the original publication. As you develop and confirm new gRNAs, please...

... at a local bar. It doesn't have to be crazy complicated! Listen to the full podcast for more great science...the goal of communicating science to the general public and training the next generation of science communicators...source of scientific information for the general public as well as a platform for scientists to be viewed...

...obstacles, and take steps to resolve interpersonal conflicts in the lab. Yearly planning meetings may not look...Paperwork. Vincent BJ, Scholes C, Staller MV, Wunderlich Z, Estrada J, Park J, Bragdon MD, Lopez Rivera...Science Career Path 7 Simple Strategies to Resolve Conflict With Difficult Supervisors Management for Scientists...

...our site. Simply click on the icon, search for answers, and choose a result. By clicking on the result links...have the option to “Leave us a message.” When you click on the "Leave us a message" button shown to the ...

...Janssen Award dinner which took place at the New York Public library. Being in the presence of scientific heroines...adaptive bacterial immunity and its potential applications in rewriting the genome. Their award acceptance... area will accelerate the launch of clinical applications. During the panel discussion, Dr. Mello took...

...postings looking for PhD applicants since many will also accept applications from master’s candidates ...technical or product support specialist,” “field application scientist,” or “scientific or technical writer...