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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 732 results for: SON

Showing: 421 - 450 of 732 results

  1. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ...Yoshimi, K., Okuzaki, Y., Gee, P., Kunihiro, Y., Sonpho, E., Xu, H., Sasakawa, N., Naito, Y., Nakada, S...INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons Additional resources on Addgene.org CRISPR ...

  4. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...Thankfully, this is a hot topic with many resources! In Bondeson et al, they review all the tags discussed here...informed choice! References and Resources References Bondeson, D.P., Mullin-Bernstein, Z., Oliver, S. et al....

  5. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    ...consider performing PEG precipitation. Instead of sonicating the cell pellet, I lyse the cells with this AAV... Stone and Harshana de Silva Feelixge at Fred Hutchinson Cancer Center for teaching me about AAV production...

  6. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    ...to capitalize on your experience. No matter the reason for attending, your preparation and day-of game...hear or understand what we are being asked. I am personally guilty of this, and have many times answered ...

  7. 3D Printing Meets CRISPR Cas9

    Type
    Blog Post
    ...Byron Rubin, a crystallographer working with Jane Richardson, provided an initial solution to this problem...are interested in experiencing Cas9 up close and personal, visit the 3D Molecular Designs web site. Or, ...

  11. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...these therapeutics. Jesse Bloom’s lab at the Fred Hutchinson Cancer Research Center developed a pooled plasmid...identified.     New from the viral service By Jason Nasse We regularly add new viral aliquots from our...

  13. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    ... figure by figure organization with a different person responsible for each figure. Others still are more... avenues including casual request via email, in person, or instant messaging but we as scientists often...

  16. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    ...philosophy of  “work hard, play hard.” Part of the reason we sincerely like spending time together is that...work” all the time. She was a wise lady for many reasons, but this saying is especially true. If your event...

  17. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...Pk1] in a western blot assay. Image reused from Anderson et al., 2024 under CC BY license.    Find ...Find Anti-V5 [IPI-SV5-Pk1] Antibody here!   Anderson, Z., Zhu, H., Riedel, T., Moshinsky, D., Meijers, R...

  18. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...proteins, and modifications to the pegRNA. The Britt Adamson Lab recently used a CRISPRi screen to identify ...useful materials for research into Alzheimer’s, Parkinson’s, ALS, and Huntington’s Disease, including plasmids...

  19. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    ... Quick tips for writing a good cover letter Personalize your cover letter to the job and job requirements...Soriano-Carot in September 2016 and was updated by Rachel Leeson in May 2024.     Additional Resources on the Addgene...

  20. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...regulatory elements or use a non-canonical code (Gustafsson et al., 2004), such as Hoogsten base pairs and...Biol 13:30–34 . https://doi.org/10.1038/nsmb1031 Gustafsson C, Govindarajan S, Minshull J (2004) Codon bias...

  21. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ...Langan, E., Siebert, S., Li, J., Greiner, D., & Anderson, D. (2015). Neutrophil Responses to Sterile Implant...  Rachel Leeson...

  22. Viral Production at Addgene

    Type
    Blog Post
    ...References Lock, M., Alvira, M. R., Chen, S.-J., & Wilson, J. M. (2014). Absolute determination of single-stranded...https://doi.org/10.1089/hgtb.2013.131 Wang, Y., Bergelson, S., & Feschenko, M. (2018). Determination of ...

  23. Lab to Office Culture Shock

    Type
    Blog Post
    ... and for a very brief period of time, the only, person to know a brand new fact is often enough to keep...learning to “speak science,” and virtually every person you talked to during the day was also a scientist...

  24. How to Write a Scientific Review Article

    Type
    Blog Post
    ...becoming an expert in your field and are the perfect person to be writing the review! Doing so is a great way...Good luck! References 1. Haery, Leila, Ryan C. Thompson, and Thomas D. Gilmore. "Histone acetyltransferases...

  25. The Challenges of Cell Culture

    Type
    Blog Post
    ...of false interpretation in cell culture for this reason. Unreproducible results are rife. Misidentified...PMID: 12001993. 4. Lucey, Brendan P., Walter A. Nelson-Rees, and Grover M. Hutchins. "Henrietta Lacks,...

  27. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    ...summarized in a short blog post, but some examples I’m personally interested in include: intracranial self-stimulation... to proceed with your behavioral experiments. Comparison of c-fos amongst animals that receive different...

  28. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...act on DNA, or are enzymatically active. For this reason, recombinant proteins are typically expressed in...culture from reaching the desired density within a reasonable time-frame. For these cases, some strains carry...

  29. Cloning

    Type
    Guide
    ...back by the 5' exonuclease. Gibson Assembly (Image from Plasmid 101: Gibson Assembly ) Back to Top Type...Engineering Guide Restriction Enzyme Gateway TOPO Gibson Type IIS Ligation Independent Cloning Oligo Stitching...cost, availability of starting materials or just personal preference. Check out our blog on choosing the... is the "classic" cloning method, and for many reasons, remains one of the most popular today. Restriction...Plasmid 101 blog post on TOPO cloning. Back to Top Gibson Assembly (Isothermal Assembly Reaction) Isothermal...Isothermal cloning, more commonly known as Gibson assembly ( protocol ), takes advantage of the properties ...lastly DNA ligase fuses DNA strands together. In Gibson assembly DNA fragments with 20-40 base pair homology...
Showing: 421 - 450 of 732 results