We narrowed to 498 results for: plasmid dna

...cite the original paper but link to plasmids from the Promega Plasmid Collection that express similar constructs...Addgene Luciferase 101 Luciferase Plasmid Collection Promega Plasmid Collection ...difficult-to-study genes. Its main function is to regulate DNA repair, senescence, and apoptosis. Work by Kamio ...cell and protein analysis products. You can find plasmids deposited by Promega scientists here.  References...

...vectors with roughly 73 million semi-random, 30 bp DNA barcodes that are integrated into target cells' genomes... ClonTracer Library at Addgene Find Lentiviral Plasmids at Addgene Check out Our Cancer Resources Page...

...self-complementing structure (scAAV) (7). As single stranded DNA viruses, AAVs normally require second strand synthesis... promote efficient transduction independently of DNA synthesis." Gene therapy 8.16 (2001): 1248. PubMed...Ready-to-Use AAV Preps Browse All of Our Viral Plasmids and Resources ...

...the assay multiple plasmids containing ITR were tested. Plasmid #59462 is one plasmid that gave reliable...any contaminating plasmid DNA carried over from the production process (DNase does not penetrate the virion... calculated using this plasmid, but will change if you use a different plasmid. Sample Calculation Size...Size in bp of Addgene plasmid #59462 : 6208 bp Concentration of Addgene plasmid #59462 : 1.07 μg/μL Molecular...consistent results. Use the recommended plasmid, or test multiple plasmids to find a suitable one. Some labs...CGGCCTCAGTGAGCGA ITR-containing plasmid for standard curve RNase-free DNase 10X DNase buffer Nuclease-free water...better results when the plasmid is linearized. Treat the purified AAV samples with DNase I to eliminate any...

...transgenic flox animals to introduce modifications at the DNA level in a temporally and locally defined manner.... share ways how scientists have used Addgene’s plasmid repository and viral service for their behavioral...

... M., Urbaniak, S. et al. Review: immunoassays in DNA damage and instability detection. Cell. Mol. Life...Resources on Addgene.org Browse Addgene’s Antibodies plasmid collection ...

...Eluted DNA 10-50 ng/μl 1 dGTP (100mM) 2.5 mM 2 DTT (100 mM) 5 mM 1 BSA (10 μg/μl) 0.25 μg/μl 1 T4 DNA polymerase... does not provide enough stability to hold the plasmid together through the transformation/replication...of its dual polymerase/exonuclease functions, T4 DNA polymerase can create overhangs of varying length...Vector Overhangs Treat the linearized vector with T4 DNA polymerase to "chew back" the free 3' ends, following...

...Collections Antibody Plasmid Collection Antibody Plasmid Collection Addgene distributes ready-to-use recombinant... table highlights plasmid collections that can be used to create and express plasmid-based antibodies....Apr 8. Markus Seeger Antibody Plasmids The table below highlights plasmids that feature expression of antibodies...) ID Plasmid Description Gene/Insert PI Return to top Do you have suggestions for other plasmids that ...collection of plasmid-based antibodies, including monoclonal antibodies, scFvs, and nanobodies. Plasmid... Plasmid Collections...recombinant antibodies made from plasmids in our collection! Many of these monoclonal antibodies are produced...

...expression vectors, DNA encoding zinc finger arrays should be cloned into plasmids also available through... Plasmid Collections Zinc Finger Consortium Zinc Finger ...

...localization dCas9 plasmid - dCas9 expression plasmid without effector fusions; 3X Flag tag; 2NLS; pCDNA3 vector ... Plasmid Collections Empty Backbones Choosing Your Perfect Plasmid Backbone Empty backbones...pYPQ203 (pMDC32-Ubi1) - Multisite Gateway T-DNA entry plasmid (attR1 & attR2); Zea mays Ubi1 promoter and... of plasmids for packaging and expression, see our dedicated Adeno-associated Virus (AAV) Plasmids page...collection of plasmids for packaging and expression, see our dedicated Lentiviral Plasmids page Retroviral...contains AdEasy®-1 plasmid View our Adenovirus Plasmids page for more information Genome...of your cells will take up your plasmid. Thus, many plasmids have markers on them so that you...

...Luciferase Plasmids Luciferase Plasmid Collection Empty Backbones Expression Constructs Reporter Constructs...otherwise noted, these plasmids are meant for expression in mammalian systems. ID Plasmid Luciferase Type(s...preparations of select luciferase expression plasmids. ID Plasmid Luciferase Type Promoter Description PI ...Resources Promega Plasmid Collection Fluorescent Proteins Guide Fluorescent Biosensor Plasmids Blog: Luminescence...suggestions for other plasmids that should be added to this list? Fill out our Suggest a Plasmid form or e-mail...of luciferase plasmids for gene expression assays and bioluminescent reporters. Plasmid... Plasmid Collections Luciferase...

... Plasmid Collections Microbiology Plasmids Microbiology Plasmids and Resources Addgene...Addgene Microbiology Resources Plasmid Kits for Microbiology : Sets of plasmids for fluorescence, cloning ...guide on assembly standards. Browse Plasmids by Microbe System Plasmids for Archaea Species Haloferax sp....Vibrio sp. Yersinia pestis Plasmids for Cyanobacteria Cyanobacteria Plasmids for Fungi Species Aspergillus...Aspergillus sp. Cryptococcus sp. Plasmids for Prions Prions Plasmids for Protozoa Species Entamoeba histolytica... tools , or by suggesting plasmids for the collection. Return to top Plasmid Kits for Microbiology Kits...P. pastoris - Gomis-Rüth Lab pOSIP plasmid kit : Integration of DNA into prokaryotic chromosomes - Endy...

...essential-but-unlauded role in the identification of DNA’s double helix. Perhaps to the dismay of middle-aged...and cheap distribution of the newest CRISPR-Cas plasmids, has been a key driver of the technology.” As ...fields, and seeing names like Feng Zhang, Jennifer Doudna, Karl Deisseroth, and Ed Boyden crop up in this...

...currently wrapping up my co-op with Addgene as a DNA Sequencing Analyst intern. As a member of the Quality...Quality Control team, I help make sure the plasmids that go out our doors are correct. Now that I am heading...

...knockout and expression construct on the same piece of DNA. We do a lot of protein-protein interaction work ...Quick, Versatile Plant Transgenesis with GreenGate Plasmids  ...

...Institute for Cell Science Plasmid Collection Allen Institute for Cell Science Plasmid Collection You May Also...full description of how to use these plasmids, check out Plasmids for Endogenous Gene Tagging in Human...Allen Institute for Cell Science has deposited plasmids to generate fluorescently tagged proteins of key...Fluorescent Protein Guide Subcellular Localization Plasmids Protocols The Allen Institute for Cell Science...induced pluripotent stem cell (hiPSC) lines. The plasmids used to generate these lines have been deposited...distribution to the research community. With these plasmids researchers will be able to generate fluorescently...Human Cells on the Addgene Blog. Plasmids for Endogenous Gene Tagging in Human Cells Find additional cell...

...use the chromatin IP (ChIP) technique to identify DNA binding proteins involved in histone modification...antibody posts  Resources on Addgene.org: Find plasmids encoding recombinant antibodies at Addgene ...

...expensive) synthetic fluorophores. Add a dash of DNA with an electrical jolt and cells become perfectly... Generate Fluorescent Protein Fusions Request Plasmids from the Michael Davidson Collection Find Fluorescent...

...selection of plasmids and tags for protein purification. The pTD Plasmid Series contains plasmids suitable...Expansion Collection Microbiology Plasmids Collection Synthetic Biology Plasmids Collection Empty Backbones ...under the microscope. The plasmids in this collection are clone-in ready plasmids that contain fluorescent...New England Biolabs Cell-Imaging Plasmid Collection contains plasmids for non-toxic protein labeling and...Optogenetics for E. coli Return to top Reporter Plasmids Reporter plasmids can be used to detect events and molecules...interactions between proteins and specific DNA sequences. ID Plasmid Reports Reporter Mechanism Expression ...CRISPR plasmids that have been designed for their use in bacteria in our Bacterial CRISPR Plasmids page ...

...Agar Plates Bacterial Transformation Recovering Plasmid DNA from Bacterial Culture Introduction This protocol... plate and select transformed cells containing plasmids differing in their resistance genes, as one does...or other antibiotic) E. coli transformed with a plasmid containing the carbenicillin (amp) resistance gene...effective at selecting only bacteria containing the plasmids of interest (for example, fewer satellite colonies...transform DH5α E. coli cells by heatshock with the plasmid of interest. See our transformation page for a ...

... non-targeting guides. Toxicity is likely due to DNA damage and its subsequent repair. Additionally, the...on Addgene.org Check out this guide to pooled plasmid libraries Find all of the CRISPR Pooled Libraries...fail to account for the effects of Cas9-induced dsDNA breaks. Are off-target cutting patterns in genome-wide...function, i.e. sites that lack open chromatin marks, DNase hypersensitivity, or are in an enhancer, transcription...control for the effects of guide expression and dsDNA breaks. Figure 2: Non-targeting vs. safe-targeting...for the inherent effects, or background noise, of dsDNA breaks caused by CRISPR. See supplemental figure...

...freezer, you’ll probably find lots of different plasmids containing the same protein of interest tagged...developed a self-labeling fusion tag based on the human DNA repair protein AGT that gets covalently linked to...Addgene’s collection, so you may be able to get a plasmid ready-to-use without any cloning at all! As mentioned...

... the mammalian brain (Wickersham et al., 2007; plasmids at Addgene). AAV vectors: A platform for delivering...neuronal cells. The AAV genome is a single-stranded DNA molecule and AAV vectors are replication deficient...involved in learning and memory (Kohara et al., 2014; plasmids at Addgene). The Gradinaru Lab at the California... throughout the brain based on its unique hue (plasmids at Addgene). A large-scale example of neuronal...than any other AAV serotype (Tervo et al., 2016; plasmid at Addgene). Its efficiency is comparable to conventional...Addgene.org Find ready-to-use AAV Preps Find AAV plasmids Browse all viral vectors ...

...aspirate supernatant. Extract genomic DNA according to the GeneJet Genomic DNA Purification Kit instructions....Thermo Scientific, 10199-452 Reagents GeneJet Genomic DNA Purification Kit, Thermo Fisher, K0721 6-well tissue...diluted and used to transduce HEK293T cells. Genomic DNA is extracted from the target cells and assayed for...green) and negative (black) droplets Sample ID Plasmid ID Dilution Copies/20 µL RRE (FAM) Copies/20 µL...

...Localization , Optogenetics Plasmids , and Chemogenetics Plasmids . Find plasmids for endogenous gene tagging... and plasmid collections that may help with your neurodegeneration research. Disease Info Plasmid Collection...Research Tools Neurodegeneration Plasmid Collection A collection of plasmids expressing wild type or mutant...more. Plasmids provided by the foundation can be found on our Michael J. Fox Foundation Plasmid Resource...genes linked to neurodegenerative disorders. Find plasmids expressing HTT, C9ORF72, LRRK2, Synuclein, and... Dev Cell. 2024) Image exocytosis with VAMP2/3 plasmids to study CNS myelination. (Lam et al. Nat Commun...Alzheimer's disease and related dementias with plasmids from the iPSC Neurodegenerative Disease Initiative...

...relatively new technology that takes advantage of DNA sequencing to turn mAb clones into expression constructs...Antibodies 101: ValidationSelecting the Right AntibodyPlasmid-based Recombinant Antibodies    ...

...Addgene CRISPR Resources CRISPR Plasmids Addgene's collection of CRISPR plasmids. CRISPR Guide Addgene's guide...HDR design; Deep sequencing Cas9: pMLM3613 dsDNA donor plasmid backbones pSL1180-HR-PUbECFP & pSL1180polyUBdsRED...guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening. CRISPR eBook Addgene's...barcoding. CRISPR Kits Addgene's collection of CRISPR plasmid kits. CRISPR Software Sanger Indel Analysis ICE... Feng Zhang Lab . Protocols Lab(s) Description Plasmids in protocol Download protocol Addgene CRISPR pooled...gRNA design and cloning for Cas9 orthologs Cas9 plasmids PDF, 107 KB Chen and Wente Zebrafish: gRNA cloning...PDF, 108 KB Fujii iChIP/enChIP to purify genomic DNA FLAG tagged dCas9 PDF, 110 KB Goldstein Nematode:...

...in analysis Plasmid-based proteins: plasmid sequence not accurate Check plasmid sequence for frameshifts...specific compartment. If your proteins are nuclear- or DNA-binding proteins, you may need to sonicate your lysates...

...of the new plasmids, antibodies, and viral preps in the repository through our hot plasmids articles. ... 2022.   Find CRISPR Csm plasmids here! Colognori D, Trinidad M, Doudna JA. Precise transcript targeting... missed something smokin'? You can pitch a Hot Plasmids section (antibodies and viral preps welcome!) ...inducible expression.   Find heat-inducible lentiviral plasmids here!   Wu Y, et. al. Control of the activity ...2: Addgene’s western blot for mEGFP-HRas (from Plasmid 18662). See the Applications section on the Anti-GFP... Campbell et al, Nature Methods   Find YFP plasmids here!   Campbell, B.C., et al. Chemically stable...novel RNA targeting system by: Susanna Stroik The Doudna lab’s recent work characterized a new tool for ...

...packaging plasmids. The cells need three different plasmids to produce AAV: 1) the RepCap plasmid which provides...going to lie, AAV transfer plasmids are kind of a pain to work with. These plasmids contain ITRs which are... AAV transfer plasmids. It’s easy to contaminate a workspace with plasmid, not that I would know about...prep. AAV samples are first DNaseI digested to remove any residual AAV plasmid that was carried over from...sample for the DNAseI digest. Use good sterile technique when handling your AAV plasmid standard and AAV...AAV’s protein shell, or capsid; 2) the pHelper plasmid which expresses adenovirus genes which help mediate...mediate AAV viral replication; and 3) the transfer plasmid which encodes a transgene of interest that’s packaged...