We narrowed to 877 results for: HA

...packaging, but their activity in mammalian systems has been in question. Three different labs have recently...CRISPR-Cas system for mammalian use Stanley Qi’s lab has developed a miniature Cas system (CasMINI) engineered...

...Cecchetelli The auxin inducible degron (AID) system has been extensively used in C. elegans since 2015 to...light-inducible CRISPR system developed by Taekjip Ha’s lab where Cas9-gRNA complex binds the target DNA...

... CRISPR 101: Una herramienta de edición  Addgene has a large international audience, including working...

...darken during the autoclave process if your sample has spent at least 10 min at 121 ℃. Use lab tape to label...

...Further, Sanger sequencing has a lower detection limit of 50-20% (although this has been improved in some ...Joung 2016 with permission. Even though progress has been made with off-target prediction algorithms, ...

...phenotypes to study. In addition, the Sánchez lab has made it easier to perform advanced developmental ...into Phagocata morgani and Phagocata gracillis, one has to purify the dsRNA from bacteria and mix the purified...

...relatively confident that the gene from the test species has a similar function to the gene in the reference species...left with Lab Director Doug Warner on the right) has taught courses in biochemistry, molecular cell biology...

...your data to Zooniverse. The Natural History Museum has downloadable resources to enable you to set up your... doors Getting involved in science communication has been the most rewarding step I have taken in my career...

...particles with polybrene, a cationic polymer. Polybrene has long been used to increase retroviral transduction...of mouse zygotes and mice with Nanoblades CRISPR has also been extensively used to create transgenic mice...

...categorization is complex Plasmid classification has shifted over the years, and new classifications are...diversity of natural plasmids and their functions has enabled researchers to develop and deposit the many...

...available on the Fragmid website, or through Addgene, has worked well in the hands of the Doench lab. Additionally...important — but so is where you’re building it! Table 1 has a list of Fragmid destination vectors currently available...

...generated with mRNA display (FingRs), Xue Han’s lab has developed AAV vectors to selectively visualize excitatory...engineered Streptococcus canis Cas9 nuclease that has a broad 5′-NNG-3′ PAM. Get the plasmids at Addgene...

...to host proteins. After the antibody of interest has been cloned into an expression plasmid, the plasmid...through open plasmid sharing, the CRISPR community has created hundreds of useful variants of Cas9. By providing...

...anyone who has ever worked to sequence DNA on NGS platforms will tell you, while the process has become much...

...including the propagation of plasmids in bacteria — has many points at which small errors can be introduced...Catching and addressing QC issues Addgene’s QC team has developed a process for identifying, categorizing...

...AAV) is a popular tool for gene delivery, but it has a drawback: how do you ensure your gene goes where...research. To improve cell-type specificity of AAV, work has focused on modifying the outside protein shell, or...

...syndrome. In response, the World Health Organization has declared a global health emergency and called for...molecular and electronic companion technologies that has transformed the assay’s capabilities. Specifically...

...training. Addgene executive director Joanne Kamens has written a 5-part blog on the topic:  http://blog....people mostly online and on monthly phone calls. This has its challenges as meeting in person is often much...

...(deposited by David Root of the Broad Institute) has consistently been one of the repository’s most frequently...Vector (deposited by George Church’s lab). Addgene has also cataloged a variety of useful molecular cloning...

...Professor Goedhart is very active on Twitter and has helped us update many of our educational resources...

...we highly recommend checking out the video). Tom has made many more Science rap videos to teach students...

...injection before performing the experiment. Every entry has an accompanying image so that you can see for yourself...

...Since rolling out our viral service in 2016, Addgene has shipped thousands of AAV and lentiviral preps to ...

...pressures during protein evolution. The Baker Lab has deposited multiple Top7 plasmids at Addgene, along...

...used in E. coli and yeast, you’re in luck! Addgene has so many of these resources that we created filters...

... crucial environmental research programs, Canada has been here before.  These attacks on science ultimately...politicians saying that climate change is a hoax. This has culminated in the current President holding and campaigning...the sacrifice in April because devaluing science has alarming implications for our public policies—many...

...inquisitive scientists. Seeing inside these specimens has offered illumination on biochemical processes that...emission of light by a substance (fluorophore) that has absorbed light or electromagnetic radiation at another...2023). The field of fluorescent ligands and dyes has witnessed remarkable advancements, driven by the ...

... of stem cell potency: Totipotency: When a cell has the ability to produce all of the differentiated ... are totipotent [2-4]. Pluripotency: When a cell has the ability to differentiate into cells of any of...

...transsynaptic spread is useful for neuronal tracing, it has two practical limitations. First, these high levels...projection neurons). Research into these phenomenon has shown that the RABV G protein is necessary and sufficient...

...useful in an experiment, and want to see if someone has already created and deposited it with Addgene. Combining...resistance gene(s) used for selection in bacteria. Has Viral Service?: Allows you to filter results to only...

...CRISPR systems for targeting ncRNAs While CRISPR/Cas9 has proven to be an extremely useful system for modifying...This system is often used to knock down lncRNAs and has fewer off-target effects. Newer Cas protein variants...

... to edit genes in several biological systems. It has been used for instance in inherited disorders such... is often used, the CRISPR approach based on HDR has the advantage of being a potentially universal treatment...

... inhibit these viruses.  But llama immunization has its drawbacks: it requires a camelid animal facility...selection step using ribosome display because it has the advantage of starting out with a larger initial...

...the tag could be quite small. However, substance P has an amide group added to its C-terminus post-translationally...and Pelham introduced the Myc tag to the world, it has become one of the most commonly used tags in molecular...

... long is the average PhD in this department? How has the department worked with students to maintain these... for everyone involved in the process. COVID-19 has changed many aspects of academia, and the PhD application...

...Although at first glance it may appear that there has to be a winner and a loser when negotiating, what... What is most important to each person? Someone has to be the first author but another author can present...

...toolbox.  References Gaudelli NM, Komor AC, Rees HA, Packer MS, Badran AH, Bryson DI, Liu DR (2017) Programmable...doi.org/10.1038/nature24644 Gaudelli NM, Lam DK, Rees HA, Solá-Esteves NM, Barrera LA, Born DA, Edwards A,...

...infect dividing and non-dividing cells.  Addgene has an extensive collection of lentiviral plasmids created... immortalized with adenoviral protein E1A, which has been shown to repress expression of HIV-1 LTRs (more...

...badge in the image below. If an abstract on PubMed has the “Find Plasmids” badge, you’ll know that within...

... started from 474 plasmid deposits in 2014 which has grown to ~10,000 plasmid deposits per year in recent...