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We narrowed to 849 results for: HA

Showing: 561 - 600 of 849 results

  3. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ...inquisitive scientists. Seeing inside these specimens has offered illumination on biochemical processes that...emission of light by a substance (fluorophore) that has absorbed light or electromagnetic radiation at another...2023). The field of fluorescent ligands and dyes has witnessed remarkable advancements, driven by the ...

  4. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    ...as they pass our standard QC process and the MTA has been approved. The spreadsheet option is recommended...cloned into the vector backbone). If your plasmid has more than one insert, click "Save and Add Another...primers can be used to verify it. If your plasmid has multiple inserts, enter details for each insert individually...

  5. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    ... crucial environmental research programs, Canada has been here before.  These attacks on science ultimately...politicians saying that climate change is a hoax. This has culminated in the current President holding and campaigning...the sacrifice in April because devaluing science has alarming implications for our public policies—many...

  10. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...transsynaptic spread is useful for neuronal tracing, it has two practical limitations. First, these high levels...projection neurons). Research into these phenomenon has shown that the RABV G protein is necessary and sufficient...

  11. Transferable Skills: Negotiation

    Type
    Blog Post
    ...Although at first glance it may appear that there has to be a winner and a loser when negotiating, what... What is most important to each person? Someone has to be the first author but another author can present...

  12. Of Myc and Men

    Type
    Blog Post
    ...the tag could be quite small. However, substance P has an amide group added to its C-terminus post-translationally...and Pelham introduced the Myc tag to the world, it has become one of the most commonly used tags in molecular...

  13. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    ...CRISPR systems for targeting ncRNAs While CRISPR/Cas9 has proven to be an extremely useful system for modifying...This system is often used to knock down lncRNAs and has fewer off-target effects. Newer Cas protein variants...

  14. PhD Applications After COVID

    Type
    Blog Post
    ... long is the average PhD in this department? How has the department worked with students to maintain these... for everyone involved in the process. COVID-19 has changed many aspects of academia, and the PhD application...

  16. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    ...infect dividing and non-dividing cells.  Addgene has an extensive collection of lentiviral plasmids created... immortalized with adenoviral protein E1A, which has been shown to repress expression of HIV-1 LTRs (more...

  19. Call for Guest Bloggers

    Type
    Blog Post
    ...development for scientists A new plasmid tool your lab has deposited If you’d like to write about a different...

  21. DNA Purification Without a Kit

    Type
    Blog Post
    ...purify DNA, but rather a glass powder slurry that has a milk-like appearance. To make DNA stick to the ...chaotropic salt sodium iodine (NaI) solution. NaI has two purposes in this protocol: 1) it solubilizes ...

  22. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...we should first note that quantitative PCR (qPCR) has been a powerful tool for quantifying AAV for some... 2 minutes and the denaturation time to 1 minute has been shown to increase droplet separation (Witte ...

  24. Lab to Office Culture Shock

    Type
    Blog Post
    ...coming directly from grad school. The transition has been a bit rough for her (3 weeks in and there's ...Scientist team says, “a well-run meeting where everyone has a voice and the group can come to a mutual agreement...

  25. How to Write a Scientific Review Article

    Type
    Blog Post
    ...requires. Using the (Last name, Year) format also has the benefit of exposing you to relevant researchers...just gotta do it." At the end of the day, someone has to write the article and that someone is going to...

  26. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    ...invention in the late 1990s, Gateway cloning technology has become very popular as a rapid and highly efficient...attL-containing entry vector.   Pro Tip: Addgene also has ready-made entry clones available for many popular...

  28. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...recombinant antibodies (rAbs) exist (though Addgene has a great collection of ready-to-use rAbs and rAb plasmids...plant, or mammalian cells. Each of these systems has their advantages and disadvantages. Briefly, bacterial...

  29. Transferable Skills Guide: Career Planning Resources

    Type
    Blog Post
    ...? There is a difference. A mentor is someone who has gone where you hope to go, and who will invest in...Joanne Kamens, a strong advocate for career planning, has written a great blog on choosing good mentors for...

  30. AAVs CREATed for Gene Delivery to the CNS and PNS

    Type
    Blog Post
    ...bloodstream allows for dispersion of the virus but it has to be able to cross the blood-brain barrier to reach...treatments for neurological diseases). The Gradinaru Lab has a lot going on. Here are the key takeaways: Two...

  31. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ...native organism.” This is possibly a better idea and has been used successfully in the past (Angov et al.,...functionally analyze the protein produced to ensure that it has folded properly. You may be able to avoid getting...

  32. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    ...University of Miami. The beginning of this century has seen some major advances in light microscopy, particularly...unprecedented detail.  One of these advances in microscopy has been light sheet fluorescence microscopy (LSFM). ...

  34. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...transcript, you will also need to make sure your plasmid has a promoter suited to working in your host organism... but requires SP6 RNA polymerase. SP6 polymerase has a high processivity. When used for in vitro transcription...

  36. Summer Fun at Addgene!

    Type
    Blog Post
    ... Addgene’s people, culture, and workplace. Rumor has it that a few Addgenies* may even be open to dancing...

  37. Our New Antibody Mascot is....

    Type
    Blog Post
    ...fact, they’ve been following a set of clues that has led them to their biggest discovery yet: Addgene ...

  39. New Viral Vectors - Spring 2025

    Type
    Blog Post
    ...newest viral vector service, AAV Packaged on Request  has seen a fantastic response from the research community...

  40. Deck the Lab 2022!

    Type
    Blog Post
    ...                     A post shared by Xi Chen (@seleena78) The lab has officially welcomed the Christmas spirit. It's just...
Showing: 561 - 600 of 849 results