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We narrowed to 929 results for: Comp;

Showing: 701 - 750 of 929 results

  2. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    ...targets.  Pre-formed primary and secondary antibody complexes (Figure 2B) Some labs have found that you can ...). One issue with this strategy is that large complexes may not permeate through your tissue samples as...

  4. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    ...interest,” Griesbeck said. Because they are chemical compounds, they have to be applied or injected, and they...give it a shot. “Beginners sometimes think it’s complicated, but actually it is very good,” he said. “It ...

  6. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    ...published an article on BioRxiv describing a comprehensive SARS-CoV-2 virus-human interaction network in... are a few key details. Our set of constructs comprises all SARS-CoV-2 viral proteins except for Nsp3 ...

  7. Celebrate National Book Month with Addgene

    Type
    Blog Post
    ...Musicophilia is accessible and fascinating, all without compromising on the relevant science."     - Randall Carter...Additional resources on the Addgene blog Learn about company culture at Addgene Read about other lunch activities...

  9. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ...and sgRNA-Sirius-8XMS2 scaffolds performed, they compared them head-to-head against CRISPRainbow. Both tools...visualization tools can be used in live cells. Compared to its predecessor CRISPRainbow, CRISPR-Sirius...

  11. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    ...custom primers or "primer-walking" reactions are completely unnecessary for sequencing multi-kb DNA inserts...seqWell, Inc. He is a sequencing technologist and computational biologist with a passion for solving critical...

  12. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    ...playing a card game called “Magic”. There were complicated rules that governed how this game was played....should be “brains-on”, but as much as I try being compelling, interactive, and application-driven to boost...

  13. The MTA at Addgene

    Type
    Blog Post
    ...has placed a request for your plasmid, they must complete a Recipient Scientist Acknowledgment (RSA), which...use for the requested material. Once the RSA is completed, the recipient organization is sent the MTA to...

  14. Hot Plasmids - January 2023

    Type
    Blog Post
    ...   Fig. 1: Csm, shRNA, and Cas13 were compared head-to-head for gene expression changes of non-target...JA. Precise transcript targeting by CRISPR-Csm complexes. Nat Biotechnol. 2023 Jan 23. doi: 10.1038/s41587...

  15. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    ...link can be your new card. Follow your contact’s company on LinkedIn (or via industry newsletters such as...this because I want it to be clear that it is completely impossible for me or anyone to stay in touch ...

  17. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    ... production of viruses used to deliver CRISPR components to cells or organisms under study. In the past...lentivirus production frustrating - a researcher can complete an entire lentiviral production protocol only ...

  19. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    ... creates a non-functional 𝛽-galactosidase. Complementing this mutation by expressing this deleted portion...get started, you will first need a primer that complements your plasmid sequence. Start with a backbone-...

  20. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    ...increase in the number of endogenous indel edits when compared to unsorted cells (Wen et al., 2018). Thus SRIRACCHA...expression. As a proof of concept the Denham lab compared editing at three different genomic sites in the...

  21. Hot Plasmids - May 2022

    Type
    Blog Post
    ...SSAP system reports an efficiency of up to 20%, comparable to commonly used CRISPR/Cas9 methods for large...error rates overall ranged from 0.3% to 10%, in comparison to the 62-90% error rate seen with Cas9 editors...

  22. Antibodies 101: Isotypes

    Type
    Blog Post
    ...portion of different antibody isotypes. Binding to complement system proteins, which can initiate a cascade...antibodies through cells and into different body compartments, such as into mucus, tears, or milk. When using...

  23. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...shRNAs are short RNA sequences which target complementary mRNA sequence for degradation. Viral introduction... virus to which immunity is desired. Instead, components of the virus are now delivered in other forms...

  25. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    ... range from a defined pathway or reaction to a complex event such as cancer or even aging. For both a ...CRISPR, drug, etc.), just with an added baseline component. Alternative screening techniques While CRISPR...

  26. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    ...binding of a new inactive heterotrimeric G protein complex and subsequently another round of signaling.    ...gamma subunits of the heterotrimeric G protein complex that can be used to detect G protein activation...

  28. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ...lentiviruses expressing various gRNAs. It’s better to compare the phenotypic effects of expressing various gRNAs...expression between experiments and over time (as compared to separate transductions with a single-vector...

  29. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    ...protect themselves from foreign DNA. The other component of these systems, methyltransferases, methylate...add methyl groups to specific DNA sequences and companion endonucleases that recognizes and cleave the same...

  31. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    ...interest is often one of the most challenging components of any immunoimaging assay. Secondary antibodies... (And while I have it on good authority that a complete Western blot can be run in a single day, I really...

  34. Addgene: The First Twenty Years

    Type
    Blog Post
    ...Addgene!  Addgene was established as an e-commerce company, which helped it stay ahead of the curve as access...combatting the virus. Our COVID-19 collection, comprised of 1302 plasmids, was shared with researchers ...

  35. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    ...important in experiments in which viability is being compromised by introduction of foreign RNAs or immunity itself... CRISPR 101: Ribonucleoprotein (RNP) Delivery Components of CRISPR/Cas9 ...

  38. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    ... about how the system operates and functions” “Complete lack of information on number of postdocs” “Postdocs...Harvard’s Therapeutics Graduate Program has a compulsory internship to be taken in an industry, regulatory...

  39. Hot Plasmids - October 2020

    Type
    Blog Post
    ...expanding our ability to study and understand the complex interworking of the 86 billion neurons in the human...Sciences. Like many split FPs, split-wrmScarlet is composed of two FP fragments that are not fluorescent on...

  41. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    .... The donor and acceptor fluorophores must be compatible, such that the emission spectrum of the donor...or be detected even if the two proteins form a complex. To help troubleshoot your experimental setup ...

  44. Design Tips for Prime Editing

    Type
    Blog Post
    ...extended to include a primer binding site (PBS) complementary to the genome and a reverse transcriptase (RT...Nureki, O. (2014). Crystal Structure of Cas9 in Complex with Guide RNA and Target DNA. Cell, 156(5), 935...

  45. Hot Plasmids February 2024

    Type
    Blog Post
    ...variant has similar photostability and brightness compared to the original, though its maturation is even...cell-specific tropism, with slightly reduced efficacy compared to AA9-X1.1. Importantly, AAV1-X1 is not neutralized...

  47. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    ...required to produce your fiber optic implant. Companies such as Thor Labs and Precision Fiber Products...writing. Derek is also a pianist and an aspiring composer. View his blog drsimonsaysscience.org or follow...

  48. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    ...practice while seated or with your poster on your computer screen than it is to pretend you’re standing at...guilty of this, and have many times answered a completely different question than the intended one. Take...

  49. Common Injection Routes in Mice

    Type
    Blog Post
    ... but they are not recommended for tumorigenic compounds, as circulation is slower, and more institutional...depending on researcher preferences. It sounds complicated but has a pretty low learning curve! Restraint...

  50. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ...plants, mammals, and insects. In this post, we will compare two of the most widely used systems, mammalian ... from dedicated adenoviral production labs or companies. Production of both AAV and lentivirus requires...
Showing: 701 - 750 of 929 results