We narrowed to 1,052 results for: FER

... been difficult to compare the performance of different nucleases. Nicola Patron’s lab compared the efficiency...genome editing toolkit New Cas protein identified Jennifer Doudna’s lab recently identified Cas14, a family... Jennifer Tsang...

...learn about intellectual property from our Tech Transfer team members Tony Cova and Chiara DeNatale and...of the students were interested to see so many different career options in science, with Abigail Anderson...professor. There are amazing opportunities that are offered with a biology major.” After hearing about many...

... name suggests, lentiCRISPR v2 is a lentiviral transfer vector used to deliver both Cas9 and your gRNA...metalloproteinase-9 secretion, and identifying lysine methyltransferase substrates. We’re incredibly happy that lentiCRISPR... forward to another great year of sharing!   References 1. Shalem, Ophir, et al. "Genome-scale CRISPR-Cas9...

...antibody. There is one major caveat - a tag may interfere with protein localization and/or function, so ... like FLAG, HA, and Myc can adopt a number of different conformations and thus potentially interact with...our older epitope tags - only time will tell! References 1. Georgieva, Maya V., et al. "Inntags: small...

...validated in.  If a plasmid's Addgene ID # has been referenced in other publications, you'll find a link to ...Additional features Once you've clicked on the "# References" link under the "Resource Information" heading...Addgene’s Plasmids 101 eBook Protocols and Plasmid References @Addgene  ...

... how our articles have helped them understand different topics of science, especially disease related ...terms like manipulations, anomaly, and so on, differently than scientists would. For example, “manipulate... receive our science career guide Learn about different careers in science communication Resources on...

...When you think about going to a scientific conference, you may think about sitting amongst a sea of chairs...ultrasound dyes because these dyes “collapse” at different ultrasound frequencies. Read more about ‘Using...communication check out these SciComm blog posts. References 1. Bourdeau, Raymond W., et al. "Acoustic reporter...

...internship would be a perfect way of exploring my different interests but also a way of challenging what I...would look like. While working remotely was a different experience, It was helpful to have Addgenies reassure...spoke with amazing people that were a part of the different departments of the company. I asked questions ...

...nervous system: infected grasshoppers expressed different proteins in their brains compared to uninfected...and geotactic behavior (Biron et al., 2005).   References Biron, D. G., et al. "Behavioural manipulation... Jennifer Tsang...

...test. These includes labs with a large number of different phages or with phages targeting organisms closely...prepare phages for clinical use. There are also different regulations from country to country when it comes... Jennifer Tsang...

...then formulate a plan to further develop your transferable skills and reach your career goals.      Although...know your strategies in the comments section!   References 1. Yearly Planning Meetings: Individualized Development... What Makes a Good Manager Anyway Developing Transferable Skills During Science Training ...

...elements. Regulatory DNA function is typically inferred based on correlative biochemical characteristics...Bioengineering from the University of Pennsylvania.     References: 1. Lee HJ, Kim E, Kim JS. Targeted chromosomal...Aug 1;289(31):21312-21324. PubMed. More CRISPR Refereces: Browse CRISPR Plasmids CRISPR Information ...

...post-its to make finding your most used protocols & references easy to find It’s happened to all of us. It’s...consider using color coded binders and tabs (for different experiments or projects) to make it easier to ...small by identifying the protocols, guides, or references that your labmates ask to borrow most frequently...

...active in E. coli and human cells Two years ago, Jennifer Doudna’s lab discovered a new Cas protein, CasX...human cells. Using the structure of CasX at eight different states of assembly from cryo-electron microscopy... Jennifer Tsang...

... to use the same peptide tag for all sorts of different protein systems and they shouldn’t get in the ...marker expression level. Labs have been trying different approaches to improve CTC capture, but I hope ...Addgene. I am a big fan; thanks for the T-shirt! References: SpyLigase peptide-peptide ligation polymerizes...

...room for serendipity during your PhD studies and offer two simple steps to welcome these unforeseen moments...project's potential. Widening your search creates fertile ground for serendipitous encounters that could ...enrich the depth and creativity of your work, while offering mental health benefits by encouraging activities...

.... Northeastern compiles a list of hundreds of different jobs for students to apply to, and after a grueling...marketing and social media co-op. There are a lot of different worries that come along with entering the workforce...of my co-op. I had to do research on loads of different best practices, create an email strategy, and ...

...in decreased efficiency of recombination and preferentially leads to gene constructs where the stop cassette...likely generalizable to other systems as well. References: del Valle Rodríguez A, Didiano D, Desplan C...plasmids at Addgene Read our Molecular Biology Reference ...

...per experiment. In the McKay lab we study how differential access to transcriptional regulatory elements...nuclei extraction. They report that this change offers a significant improvement and simplification of...cell or tissue preparation.   All in all, CUT&RUN offers a great alternative to ChIP-seq, especially in ...

...meaningful changes in the lab could make a big difference in our global impact as scientists. Many scientists... single-stream or city recycling services don’t offer plastic recycling, specialized companies can recycle...Sustainable Practices to the Lab: Easy Action Items References Geyer, Roland, Jenna R. Jambeck, and Kara Lavender...

...were really leader-specific. How is leadership different from good management? Aren't all career skills...because it emphasizes the importance of these very different roles. At any stage in our careers we may be in...boss) that you are ready to try operating in a different role?  I think the secret sauce can be summed ...

...feedback (may be the same as self-assessment or different): Here are the 3 things I would say worked out...you needed? What could I (manager) have done differently? What Can You Learn Going Forward: If you could...could do the thing all over, what would you do differently? Beware of indirect language You must be honest...

...want to offer a huge thanks to all the Addgenies who worked so hard to make this poster, offered suggestions...of the biggest names in the CRISPR-Cas9 field – Jennifer Doudna, Feng Zhang, and Keith Joung. All Addgene...

...in this series. Check out Joanne's Reddit AMA Different types of mentors for grad school vs. postdoc Choosing...Choosing an advisor to complete a successful PhD is different from choosing a lab for postdoctoral work. However...think, “Oh, but that won’t happen to me – I am different.”  Unfortunately, a bad mentor can be bad news...

...genes) system. This popular genome engineering tool offers flexibility, multiplexibility, and ease of use....moieties carry out these functions using slightly different Cas proteins and PAM sequences. A total of eight...involves CRISPR/Cas technology? Let us know.  References Ishino Y, Hideo, S, Makino K, Mitsuko A, Nakata...

...)    Science communication, or science writing, refers to the process of communicating science to laypeople...knowledge or interest in STEM subjects; we generally refer to them as the “interested public.” Subscribers ...field-specific terminology or language. This can refer both to terms that are only used in the field and...

...Ready to start epitope tagging?   References and Resources References Terpe, K., Overview of tag protein...obvious con of GFP is its size – which can possibly interfere with protein function and localization. If you...

...will help with the next steps.   References and resources References Al-Allaf FA, Tolmachov OE, Zambetti...Choose a restriction enzyme that will give you different band patterns for the recombined vector backbone...

...diseases. “Today, I am a scientist that helps with different aspects of manufacturing and quality, especially...microRNA-based system for polymerase II-regulated RNA interference. In the first two months since publication, ... Jennifer Tsang...

... are fused to. The researchers constructed 22 different mTFP1 fusions to both the C- and N- termini of...community, please deposit the plasmids at Addgene! References Ai, Hui-wang, et al. "Directed evolution of a... Jennifer Tsang...

...combinatorial expression of these three genes with different copy numbers, producing dozens of color hues (... using in utero electroporation. Then at your preferred time points, sacrifice the animals and fix the...website: https://dn-en.med.kyushu-u.ac.jp/     References Cai, Dawen, et al. "Improved tools for the Brainbow...

...enable the conversion of RNA templates to DNA but suffer from several important shortcomings including low... containing an anti-GFP nanobody fused with a different functional element (tyrosine sulfation consensus...APEX2), allowing for quantitative analysis using different detection methods. Since the anti-GFP nanobody...

...topoisomerase I (1). If a 5′ hydroxyl group from a different DNA strand comes along, it can attack this covalent...Gel purify your PCR product and incubate it with buffer, Taq and dATPs at 72C for 10-15min.  When mixing...may allow you to see colonies within 8 hours. References: 1. Shuman S. "Recombination mediated by vaccinia...

...field is a series of fluorescence resonance energy transfer (FRET)-based calcium biosensors. Perhaps most ...enable observation of the interaction between different molecules like zinc and calcium. Ultimately, Merkx... start using it. For us, it’s like a test.” References Thestrup et al. Optimized ratiometric calcium...

...remote collaborations Transferable Skills Guide: External collaborations Transferable Skills Guide: Teamwork...ask questions if you don’t understand something. Offer to help out wherever you can and don’t forget to...

...media, etc.). The free version of Grammarly also offers basic writing suggestions, like alternative word...same features, but this chart highlights the differences between these two citation managers. If you want...a favorite writing tool, let us know below! References and Resources You Can Grad School podcast episode...

...     - Randall Carter, Contracts Administrator Inferior: How Science Got Women Wrong - and the New Research...sometimes we benefit and sometimes we don't."     - Jennifer Tsang, Science Communications and Marketing Coordinator...follows an intriguing cast of characters from five different families over several millennia as their paths...

...developed, such as the Forster resonance energy transfer (FRET)-based executioner caspase reporters. In...in living animals. To do this they modified a different self-assembling split GFP (GFP1-9 and GFP10-11...plasmids for FlipGFP and FlipCherry at Addgene. References Cabantous, Stéphanie, et al. "A new protein-protein...

...there are two overlapping, valid critiques being offered of He’s work: CRISPR/Cas9 genome editing is not...possibility, safety, and efficacy of the mtDNA transfer options being investigated; A UK regulatory body...self-police” or whether the media and academic conference response to He created a positive feedback loop...

...are, how complicated (and again, expensive) the different machines are, and how many regulations are in ...advocacy. You can email her at [email protected].   References 1. Huang, Ally, et al. "BioBits™ Explorer: A ...lab techniques Check out our molecular biology reference pages Find plasmids from the BioBits BrightTM ...

...sequences and lineages, FRET efficiencies, additional references, etc...) and a more robust database structure...address).  One may also simply attach important references that serve to further characterize a protein ...Currently, all submissions also require a DOI referencing primary literature, and every effort is made ...

...both individual and community science projects, we offer classes, workshops, book clubs, seminars, and social...the data to characterize the microbiomes of the different samples. The goal is to not only learn something...something about the microbial diversity in different soil conditions/locations but to engage the local community...

...assembly? In the context of cloning, DNA assembly refers to a method of physically joining multiple fragments...generate the mutation and linearizing your vector (preferably by PCR) for DNA assembly. Further, there is no...assemblies and improvements in these methodologies offer streamlined and simplified alternatives to traditional...

...used a solution of cell culture medium, which is buffered and contains the pH indicator phenol red. We froze...permeable to CO2 at low temperatures. We tested two different types of tubes: the ones we use to store lentivirus...also using the thawed virus within 24 hours. References 1. Murphy BM, Swarts S, Mueller BM, van der Geer...

...hundreds of different plasmids from a single Illumina MiSeq® run. As shown in Fig. 2, below, differences in input...constructs like plasmids. The plexWell™ workflow also offers a highly efficient NGS-based alternative to Sanger...

...sure to thank everyone for their participation and offer encouragement for the next steps (see “action items...employee’s workload, updates on current projects, and offer feedback. The employee should ask for input on projects...-size-fits all. Feel free to play around with different strategies and find out what works for your organization...

...blog post we will discuss how mammalian plasmids differ from their bacterial counterparts, including how...selection markers are listed below: Name Gene Conferring Resistance Cell Types* Mode of Action** Working.... This can be confusing since the neo/kan gene confers G418 resistance; however, like gentamicin, neomycin...

...explaining more of her role, which eventually led me to transfer to her team and begin my product management (PM... part of my job is getting to talk to so many different people! I communicate with customers, software... get software product management internships, preferably on teams that run within the agile framework ...

... ideas come from social media conversations, conferences, and questions our scientific support staff receives...line with Addgene style.        Fig. 2: Reference image (left) provided to our graphic designer ...constantly reviewing the resources we currently offer to identify gaps and add to them as techniques evolve...

...experiment moving. Happy editing! References and Resources References Hendel, A., Bak, R., Clark, J. et...used in CRISPR-Cas9 experiments – the “gRNA” – suffers from the same issues as your experimental RNA. ...