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We narrowed to 929 results for: KIN

Showing: 701 - 750 of 929 results

  1. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    ... blue, indicating that your IPTG and x-gal are working as they should be. Don't rush the process: It...between blue and white colonies. Take care in making your plates: X-gal is light and temperature sensitive...

  2. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...conserved cellular machinery to process gRNAs. Hijacking tRNA processing to make gRNAs In a recently published...Cas toolkit in human cells. Nissim L, Perli SD, Fridkin A, Perez-Pinera P, Lu TK. Mol Cell. 2014 May 22...

  3. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    ...of the same technical advantages that come with working with prokaryotes. This includes but is not limited...selection rather than antibiotic selection when working with yeast, due to high rates of spontaneously ...

  4. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    ... cardiovascular and metabolic diseases. We are knocking out genes that have been implicated in disease...about the history. How had investigators done this kind of work before? Musunuru: In the "olden days," not...

  5. AAV Production in HEK293 Cells

    Type
    Protocol
    ...After 2 months, discard the tube and thaw a new working stock. 40% POLYETHYLENE GLYCOL (PEG) 8000 solution...

  6. Summer SciComm: Preprints

    Type
    Blog Post
    ...unpublished results at a conference? Post your working manuscript as a preprint too and you can share ...American Society for Cell Biology in a position working with preprints in life sciences. He also posted...

  7. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    ...proteins in your assay.  Narrowing the field After working your way through the above steps, you may find ...an antibody website. In 2016, the International Working Group for Antibody Validation proposed 5 pillars...

  8. What's New in CRISPR - November 2021

    Type
    Blog Post
    ...developed by engineering the Cas12f scaffold gRNA and making strategic mutations in the DNA binding cavity of...half the size of the Cas9 or Cas12a based systems, making it well suited for AAV packaging. Most importantly...

  10. Addgene: The First Twenty Years

    Type
    Blog Post
    ...people knew how to access Addgene, and that we were making it possible for scientists to both deposit and ...through physical outreach, visiting organizations, knocking on doors, and attending conferences. And when ...

  14. Hot Plasmids - October 2020

    Type
    Blog Post
    ... ability to study and understand the complex interworking of the 86 billion neurons in the human brain... opens the door to in vivo experiments such as tracking the synaptic maturation pattern of hippocampal...

  21. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    ...detection We and other members of the lab had been working on advancing the paper-based platform toward a ...when we needed additional expertise. Perhaps most striking is that there were seven institutions involved...

  22. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    ...has slightly different activation/inactivation kinetics. For example, the light-gated cation channel, ...protein-coupled vertebrate rhodopsin in cerebellar Purkinje cells. The Journal of biological chemistry. 2011...

  24. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...setup. This requires a less involved setup when working with mice and slightly less red tape for approval...has psuedotypes. Psuedotyping is the process of making a hybrid AAV, with the genome from one serotype...

  25. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ... fluorescent proteins.  Currently, the team is working to identify how MagLOV’s structure changes under...starting point for much more. If you’re interested in working on these questions or collaborating, the York Lab...

  26. Pooled Library Amplifications

    Type
    Blog Post
    ...bacteria can grow and compete in the same container, making it easier for a smaller, recombined plasmid to ...developing your amplification protocol! We recommend looking at protocols used for libraries that are similar...

  27. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    ...binding distinct PAM sequences. Now, Hu et al., working in David Liu’s lab, have gone one step further,...Once you’ve tried it, let us know how xCas9 is working for you in the comments section! References Hu...

  28. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    ... side, successful indel generation and epitope knockin was achieved by a few respondents suggesting that...inconvenient. Another possible reason for the trickiness of NgAgo is the inability of mature NgAgo protein...

  30. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    ...don’t require a PAM sequence at the target locus, making them more flexible than Cas9/Cpf1. Some Cas13 enzymes...prefer targets with a given single base protospacer flanking site (PFS) sequence, but orthologs like LwaCas13a...

  32. DNA Purification Without a Kit

    Type
    Blog Post
    ... bonds with water molecules and coaxes it into sticking to the hydrophobic glass filter. Each glass filter...depending on the size of bacterial culture you’re working with. Overall, the glass syringe filter method ...

  33. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...culture experience. Let’s go over the basics of making rAbs. Production Recombinant antibody production... because it secretes fewer of its own proteins, making the purification process simpler. Due to its high...

  34. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ...Ampicillin, often just called amp, is the historical king of the antibiotic resistance realm. Amp is a semisynthetic...period after transformation (the dreaded 60 minutes shaking at 37 degrees) can be shortened since the plated...

  35. MXS Chaining

    Type
    Blog Post
    ...475nm Lyn-tag (derived from the tyrosine-protein kinase Lyn) Membranes 3 mCherry 587nm/ 610 nm human ...Anderson JC, Dueber JE, Leguia M, Wu GC, Goler JA, Arkin AP, et al. BglBricks: A flexible standard for biological...

  36. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    ...the transformation efficiency of the strain: when asking the cell to perform multiple recombination events...recommend Phusion or Q5. Speed up your life Rather than making up your PCR mix every time, pre-made aliquots of...

  38. Plasmids 101: Colony PCR

    Type
    Blog Post
    ... insert-specific sequence. This is a “yes or no” kind of test, with a positive clone amplifying a product...this type of primer pair anneals to a sequence flanking the insert and one primer anneals to the insert...

  39. CRISPR Antimicrobials

    Type
    Blog Post
    ...and public health and medical organizations are working to help educate the public about proper antibiotic...observe any mutations at the target site. In a mouse skin colonization model, phagemid treatment decreased...

  40. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...library screen only to find the system itself is not working because your reporter protein doesn’t function ...Rajagopala S.V., Stellberger T., Uetz P.  Exhaustive Benchmarking of the yeast two-hybrid system. Nature Methods...

  41. A Career in Grants: How To Become a Grant Professional

    Type
    Blog Post
    ...and develop an area of expertise. Whether you’re seeking a full-time job or want to be an independent grant...a mentor, find your people Find someone who is working or has worked as a Grant Professional to mentor...

  42. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ...optogeneic actuators and sensors at Addgene. Cell marking/selection: Expression constructs like plasmids ...Because of its stability, GFP can be used in lineage tracking capacities in cell fate studies. It can also be...

  43. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    ...many questions from scientists all over the world looking for some additional information or clarification...functional before investing time and resources into making a stable cell line. When expressing a viral construct...

  44. Hot Plasmids - August 2020

    Type
    Blog Post
    ...the yeast extracellular surface) which enables tracking of protease activity with a fluorescent protein...that the best motif was from the Lck gene. For marking microtubules of mammalian cells, the author found...

  45. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ... Specific High Sensitivity Enzymatic Reporter UnLOCKING (SHERLOCK and SHERLOCKv2) (Gootenberg et al., ... Specific High Sensitivity Enzymatic Reporter UnLOCKING Cas13 (C2c2) was first identified in 2016 by the...

  46. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...tools for cellular targeting. Lately, they’ve been working on two tools that expand and exploit CRISPR, both...Jonathan and experts in CRISPR evolution, were also working on the project. Using computational observations...

  49. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    ...research vision uninterrupted and then you will begin working through your aims over the next 45 minutes. The...salient details. Make the most of your limited speaking time. Beyond the information you seek to convey...

  50. Antibodies 101: The Four ELISAs and When to Use Them

    Type
    Blog Post
    ...The assay only uses one antigen binding antibody, making it less specific than other methods. In addition...positive control will confirm that the procedure is working and should always be included.  For a negative...
Showing: 701 - 750 of 929 results