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We narrowed to 1,014 results for: REN;

Showing: 701 - 750 of 1014 results

  1. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    ...splicing, imaging, and more. Abil says she is currently working on a version that she hopes will change...PUF proteins lose their solubility. Her lab is currently investigating the issue and hopes to have a better...happy to share updated versions of the kit.” Reference Abil Z, Denard CA, Zhao H.  Modular assembly of...

  2. Developing Transferable Skills During Science Training

    Type
    Blog Post
    ...colleagues you help will be future advocates and references. Collaborate with another lab, in another field... a committee and do something big like plan a conference Recruit many supporters and mentors that will...skills too. Teach classes Go to workshops and conferences on interviewing, resume writing, and networking...

  3. Let There Be LITE Plasmids

    Type
    Blog Post
    ...the researchers applied the LITE system to 28 different genes to establish it as “a novel mode of optogenetic...activators are easy to expand or combine with different effectors. I’d encourage people to get creative...readers, how will you get creative with LITE? Reference: Silvana Konermann et al. "Optical control of ...

  4. Science Guides

    Type
    Guide
    ...Pooled libraries are single preparations of many different plasmids. Plasmids within a given library have...the same backbone, but they express or target different genes. Some libraries cover the majority of the...Find educational guides to learn more about the different types of viruses that are commonly used in research...

  5. Stepping into a Virtual Internship: My Experience at Addgene

    Type
    Blog Post
    ...internship would be a perfect way of exploring my different interests but also a way of challenging what I...would look like. While working remotely was a different experience, It was helpful to have Addgenies reassure...spoke with amazing people that were a part of the different departments of the company. I asked questions ...

  6. Six Spooky Science Stories and Halloween at Addgene

    Type
    Blog Post
    ...nervous system: infected grasshoppers expressed different proteins in their brains compared to uninfected...and geotactic behavior (Biron et al., 2005).   References Biron, D. G., et al. "Behavioural manipulation...Isopoda)." Copeia 1983.3 (1983): 813-816. Gilbert, Lawrence I. "Halloween genes encode P450 enzymes that mediate...

  8. Searchable and Sortable gRNAs for Your Next CRISPR Experiment

    Type
    Blog Post
    ... gRNA table You can access the datatable here. Current table columns are target gene, species, sequence...ID, and depositor. The CRISPR applications we currently have data for are: cut, activate, interfere, visualize...jointly by Brook Pyhtila and Nicole Waxmonsky. References McDade J, Waxmonsky NC. Emerging CRISPR Technologies...

  10. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    ... might not fold well, you should screen a few different vectors to see which gives you the most functional...two sets, each of which includes plasmids with different varieties of tags, both N- and C-terminal. Each...individually or as a complete kit from Addgene. References 1. Goulas, Theodoros, et al. "The pCri system...

  11. Science as Art

    Type
    Blog Post
    ...LA, where it is currently on display.   Many universities are now following the trend and encouraging... biology and I believe it could have made the difference in some areas of conservation.” Appreciating ...

  12. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

    Type
    Blog Post
    ... by Daniel Bauer and Matthew Canver of Boston Children’s Hospital and Harvard Medical School. Addgene ...hematologist and principal investigator at Boston Children’s Hospital and Harvard Medical School. His lab ...Bioengineering from the University of Pennsylvania.     References: 1. Lee HJ, Kim E, Kim JS. Targeted chromosomal...

  13. Inside Addgene's 10 Year Anniversary Gala

    Type
    Blog Post
    ...Addgene grow into the successful, internationally renowned plasmid repository that it is today. Although ...faces of some of our depositing scientists who weren’t able to join us in person. Joanne Kamens, Melina...entire night was when Melina and Ken called their parents up to the podium to be recognized. John and Stephanie...

  14. Celebrating Accomplishments in the Lab

    Type
    Blog Post
    ...lots of interesting things from children, their classmates, and parents”. He also cooks and cleans with...academics because it offers more flexibility and you aren’t locked into a schedule.” George Church comments...

  15. Three Tips to Organize Your Lab Notebooks in the New Year

    Type
    Blog Post
    ...post-its to make finding your most used protocols & references easy to find It’s happened to all of us. It’s...consider using color coded binders and tabs (for different experiments or projects) to make it easier to ...small by identifying the protocols, guides, or references that your labmates ask to borrow most frequently...

  16. 2023 and 2024: Reflections and Looking Ahead

    Type
    Blog Post
    ...With renewed energy and optimism that comes at the start of every new year, it is a wonderful opportunity... from 1,106 labs in 504 organizations from 37 different countries, into our repository, while expanding... We will pursue opportunities to build on our strengths that have successfully served the scientific community...

  17. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    ...assistance with this blog post. References and resources References Feng, J., Zhang, C., Lischinsky, ... nLight nLight sensors are based on alpha-1a adrenergic receptor; nLightG contains circularly permuted...

  18. Supporting Rare Disease Research with BeHeard 2017

    Type
    Blog Post
    ...in diabetes and lymphoma research or the many different ways you can improve your heart health. These .... Like other forms of mesothelioma, there are currently no targeted treatments for peritoneal mesothelioma...rare disease research funding opportunities. References 1. Jones, C., et al. "PP09. 1–2352: Systematic...

  19. SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture

    Type
    Blog Post
    ... to use the same peptide tag for all sorts of different protein systems and they shouldn’t get in the ...marker expression level. Labs have been trying different approaches to improve CTC capture, but I hope ...Addgene. I am a big fan; thanks for the T-shirt! References: SpyLigase peptide-peptide ligation polymerizes...

  20. London Calling - Addgene’s New Europe Office in London

    Type
    Blog Post
    ... more than 75,000 plasmids to more than 1,500 different institutions in Europe. In the last four years...over the first five months of 2014 reaffirms this trend. In addition to this, more and more European scientists...focused on supporting European scientists. Born a french frog, I moved to Albion six years ago to work as...

  21. CRISPR 101: Drug discovery and CRISPR

    Type
    Blog Post
    ... the biomedical research field! References and Resources References Fellmann, C., et al., Cornerstones...knockout in a cancerous background and treat the parental cell line and the X knockout with your drug(s)...

  22. Plasmids 101: What is a plasmid?

    Type
    Blog Post
    ...fundamentals. Our mission is to curate a one-stop reference guide for plasmids, so that you can spend less...plasmid use in the lab. Addgene’s Molecular Biology Reference includes information about molecular cloning, ...a plasmid vector, molecular biology tools and references, and how to maintain your plasmid stocks. The...

  23. Oh, The Places You Can Go: Careers in Science Communication - Product Development

    Type
    Blog Post
    ...science writing is clear, Caitlin’s career plans weren’t concrete when she finished grad school at the University...enjoyed the writing and editing sphere.” But things aren’t always that simple, especially when your spouse...growing in the science sphere, but the journals aren’t out here. They’re really East Coast. When we first...

  24. FastCloning: A Newer, Simpler Cloning Technique

    Type
    Blog Post
    ...guest blogger, Meghan Monroy. Meghan Monroy is currently an Outreach Data Curation Intern at Addgene. She...Connecticut interested in protein science.     References: 1. Celie, Patrick HN, Annabel HA Parret, and...Recombinant cloning strategies for protein expression." Current Opinion in Structural Biology 38 (2016): 145-154...

  25. Management for Scientists: Giving Feedback

    Type
    Blog Post
    ...feedback (may be the same as self-assessment or different): Here are the 3 things I would say worked out...you needed? What could I (manager) have done differently? What Can You Learn Going Forward: If you could...could do the thing all over, what would you do differently? Beware of indirect language You must be honest...

  26. Choosing a Good Mentor for Scientists

    Type
    Blog Post
    ...in this series. Check out Joanne's Reddit AMA Different types of mentors for grad school vs. postdoc Choosing...Choosing an advisor to complete a successful PhD is different from choosing a lab for postdoctoral work. However...think, “Oh, but that won’t happen to me – I am different.”  Unfortunately, a bad mentor can be bad news...

  27. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    ...out if you want to learn more. References and resources References Balloux, F., & van Dorp, L. (2017...and an enveloped virus (right). Created using biorender.com   If you’re new to talking about viruses, here...

  28. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    ... together as useful and productive, forming a different constellation of interests and skills than what...Advancement of Science (AAAS), we have all sorts of different editors. Do you like science journalism? Take ...biology. Beyond that, she is also a fan of baseball, French horn, and rhubarb. Follow her on Twitter @Pam_Hines...

  30. Meet the New Addgene Board Members!

    Type
    Blog Post
    ...diseases. “Today, I am a scientist that helps with different aspects of manufacturing and quality, especially...microRNA-based system for polymerase II-regulated RNA interference. In the first two months since publication, ... and former Associate Director of Viral Vectors Karen Guerin for the journal Molecular Therapy. Vandenberge...

  32. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    ...why this protein was blue-shifted compared to its parent protein CFP484. Collaborating with scientists from... are fused to. The researchers constructed 22 different mTFP1 fusions to both the C- and N- termini of...community, please deposit the plasmids at Addgene! References Ai, Hui-wang, et al. "Directed evolution of a...

  33. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type
    Blog Post
    ...combinatorial expression of these three genes with different copy numbers, producing dozens of color hues (...2013). However, the brightness of Brainbow was inherently low. This is because the stochastic and combinatorial...website: https://dn-en.med.kyushu-u.ac.jp/     References Cai, Dawen, et al. "Improved tools for the Brainbow...

  34. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ...initial system by using a stronger promoter and different biochemical secondary energy sources to increase...XMAS-TREE. a) Cells are transfected with three different vectors and flow cytometry is used to sort populations...XMAS-TREE-based methods at various genomic loci in four different categories.     Brookhouser, N., et al. BMC...

  36. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    ...sections and brought in more focus on the list of conferences I presented at and placed them under headlines...before I went for the interview. So reading for my current sales position interview was just as important....mechanisms of cell division. Recently Seán got the entrepreneurial bug and has set up an ELISA kit company on ...

  37. Guide to Using Pooled Libraries

    Type
    Guide
    ...pooled libraries are single preparations of many different plasmids. Plasmids within a given library have...the same backbone, but they express or target different genes. Some libraries cover the majority of the... library from the Moffat lab has over 175,000 different gRNA-containing plasmids). Amplifying and Using...

  38. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

    Type
    Blog Post
    ...academic community by funding their travel to a conference of interest. In this post, we’re proud to announce...for human milk oligosaccharides Fatima Enam is currently a graduate student in Thomas J. Mansell’s lab ...cost-effective way to measure the production of HMOs. Current HPLC-MS and fluorophore-based technologies are ...

  39. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    ...are, how complicated (and again, expensive) the different machines are, and how many regulations are in ...advocacy. You can email her at [email protected].   References 1. Huang, Ally, et al. "BioBits™ Explorer: A ...lab techniques Check out our molecular biology reference pages Find plasmids from the BioBits BrightTM ...

  42. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    ... improve tuberculosis care and control. In her current research, Sheen aims to identify new genes related...related to PZA-resistance by using different approaches, including CRISPR and recombination-mediated genetic...microfabrication technologies to generate a self renewable sensor, a bacterium that can sense contaminants...

  43. How to Lead a Great Meeting

    Type
    Blog Post
    ...new and useful information, and invigoration - a renewed energy for continuing the project after leaving...a sense of the employee’s workload, updates on current projects, and offer feedback. The employee should...-size-fits all. Feel free to play around with different strategies and find out what works for your organization...

  44. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    ...inactive state. The dynamic range, defined as the difference between the lowest and highest level of “output...-friendly. They experimented with the promoter strength of various components, removing inducible promoters...various scientific or engineering applications.   References Refactoring and optimization of light-switchable...

  45. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...Viral Vector Resources at Addgene! References and Resources References Travieso T., et al. The use of viral...Biological Tools blog can help you learn about the different types of viruses and pick one for your experiment...

  46. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ...can lead to variable expression levels due to differences in copy number.  And there are the usual transfection...Method Delivery Method Advantages Disadvantages References Yamamoto Lab Golden Gate Assembly transfection...multiplexing, leave them in the comments below.        References 1. Zetsche et al. "Multiplex Gene Editing by ...

  47. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type
    Blog Post
    ...have taken the CRISPR world by storm. Like RNA interference, these enzymes can knock down RNA without altering...multiple RNA regulating methods: small hairpin RNA interference, dCas9-mediated transcriptional inhibition (...their tools available to the broader community. References Konermann, Silvana, et al. “Transcriptome Engineering...

  48. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

    Type
    Blog Post
    ... to its involved in cell proliferation and differentiation.  Since Ras is a membrane localized GTPase,...expression changes lead to cell proliferation and differentiation. The lab confirmed this OptoNB-SOS activated...for more uses both inside and outside cells. References Gil, Agnieszka A., et al. "Optogenetic control...

  49. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...introducing mutations (red nucleotide). Created with BioRender.com. The initial version of EvolvR has an editing...the system and the flexibility to address many different types of research questions. Are you ready to ...experiments? Find EvolvR plasmids at Addgene! References Gaj, Thomas, and Pablo Perez-Pinera. "The continuously...

  50. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...expression, PTGs will likely be adaptable to many different biological systems. Although Xie et. al characterized...in plants, PTGs will likely work well in many different biological systems. Size is also a factor; the...technology, leave them in the comments below.  References Boosting CRISPR/Cas9 multiplex editing capability...
Showing: 701 - 750 of 1014 results