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  2. Save Time and Money by Making Your Own Competent Cells

    Type
    Blog Post
    ...protocol has worked well for us for multiple E. coli strains, including DH5alpha, XL1 Blue, TOP10, ccdB survivalTM...competent cells, then you're best off following the instructions that came with them. If you decided to make ...

  3. Let There Be LITE Plasmids

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    Blog Post
    ...lab at MIT, the use of customizable DNA-binding domains based on transcription-activator-like effectors...control gene expression in mouse neurons and in the brains of living mice both quickly and significantly. ...

  4. What's New in CRISPR - December 2019

    Type
    Blog Post
    ...bionanoparticle system for packaging Cas9/gRNA ribonucleoproteins for transient expression. They replaced the...ADAR2 into a cytidine deaminase. This editor still retains A-to-I editing. This system is called RNA Editing...

  6. Modulate the Activity of 17 Signaling Pathways with One Kit!

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    Blog Post
    ...Kathleen Ottina and David Sabatini at the Whitehead Institute and MIT, started with a list of 17 signaling pathways...pathways. With support from the National Cancer Institute, they expect to add another 200 or 250 constructs...

  7. Management for Scientists: Seeking Feedback

    Type
    Blog Post
    ...series on Management for scientists. This second installment in the Management for Scientists series will ...Walking Around" – don't hide in your office. Stop by instead of emailing.    Demonstrate you are open to feedback...

  9. X-CHIME: Context Dependent Germline Knockout in Immune Cells

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    Blog Post
    ...suite of four systems which utilizes custom mouse strains and lentiviral expression vectors (Table 1). The...generation of exhausted CD8+ T cell subpopulations and restrains tumor immunity. Nature Immunology 20, 1335–1347...

  13. 2023 and 2024: Reflections and Looking Ahead

    Type
    Blog Post
    ...June, we announced a new partnership with the Institute for Protein Innovation (IPI), in which Addgene.... The first IPI collection, 31 rAbs targeting integrins, will be released early this year.  In September...

  15. London Calling - Addgene’s New Europe Office in London

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    Blog Post
    ...75,000 plasmids to more than 1,500 different institutions in Europe. In the last four years alone, the...fantastic numbers, a lot of European scientists and institutions are not aware of what Addgene can do for them...

  17. New CRISPR Web Resources and #12DaysofCRISPR Recap

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    Blog Post
    ...demethylation by Tet1. RNA Targeting: This page contains Cas13 constructs for mammalian and bacterial systems...off-target effects! This characteristic probably explains why pC016 - LwCas13a guide expression backbone...

  19. New Viral Vectors - Fall 2024

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    Blog Post
    ...If the fall colors are inspiring you to paint your cells in equally vivid colors, you'll want to check...vectors from the Jonathan Ting lab at the Allen Institute, deposited as part of the BRAIN Armamentarium ...

  20. An Addgene Summer Internship

    Type
    Blog Post
    ...toxin, or an oncogene, for safety reasons, and the insert size should be a max of 500 bp (preferably). I ...college and play the drum set as their personal instrument. They are the oldest sibling in a Guatemalan ...

  21. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    ...Senior Director of Biology, is helping us fight against FOP in two ways. First, Addgene is distributing...plasmids with Addgene and join us in the fight against FOP. Second, Addgene will be hosting the AJ’s Boston...

  22. Addgene at the ASM Microbe 2016 Conference

    Type
    Blog Post
    ...one of the highlights of the conference. It was inspiring to see how microbiology research is improving ...symposia outlined the fantastic work this Broad Institute lab is doing creating well-optimized CRISPR screening...

  23. 10 Ways to Share Your Science!

    Type
    Blog Post
    ...out to science writers in your area or at your institution; the news or communications office is a good ...internship at a place like Science News. Ask your institutional news office if there might be opportunities ...

  24. Advice for Choosing a Research Project

    Type
    Blog Post
    ... your professional brand. It offers colleagues insight into your interests and will determine how you ...for guidance, intelligent discussion, and/or brainstorming sessions to maximize productivity and direction...

  25. CRISPR/Cas9 FAQs Answered!

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    Blog Post
    ... What is the maximum amount of DNA that can be inserted into the genome using CRISPR/Cas forHomologous...efficient recombination? A3: The most we've tried to insert so far has been 1kb. We used homology arms that...

  26. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    ...Instead of spending time and money producing virus from select vectors in the repository yourself, you...serotypes that can be used to deliver fluorescent proteins to cells as determined by the serotype. You can...

  30. Intro to Our Summer SciComm Series!

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    Blog Post
    ...field of science you are communicating from. For instance, a plumber, a physicist, an ambassador to Spain...exclude people outside of the original recipients. Instead, it targets a specific audience - giving consistency...

  32. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    ...sensors, and other common neuroscience tools into the brains of model organisms, including the Anikeeva lab’... Plasmid tools for microbiome studies Channel rhodopsins for optogenetics Viral Vectors 101: Chemogenetics...

  33. Science Careers: Unruly Interests Feed Many Paths

    Type
    Blog Post
    ...embryogenesis. My interests, though, have proven unruly. Instead of settling down into a singular focus on molecular... general terms on a couple of very broad topic domains, but each day brings in new and surprisingly diverse...

  34. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    ...partnerships with universities, companies, hospital institutions and funding bodies. Don't shy away from the ...company advancement. Be relentless The world is against you.  As I mentioned, you start at a disadvantage...

  35. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    ...help unlock the potential of modern sequencing instruments by enhancing the efficiency and simplicity of...completely unnecessary for sequencing multi-kb DNA inserts and larger targets. The NGS readout also reveals...

  36. Plasmids 101: NGS Quality Control for Pooled Libraries

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    Blog Post
    ... the DNA prep is larger. And since the library contains many plasmids, care must be taken to ensure that...plasmids in the library are identical except for the insert; there’s no need to sequence other parts of the...

  38. Summer SciComm Series: A PhD in Science Communication

    Type
    Blog Post
    ...never finish a course of antibiotics, choosing instead to save a few days’ worth of capsules to self-medicate...much of the information gained from that research remains inaccessible, behind paywalls and in technical ...

  40. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    ...models for immunogenicity prediction of therapeutic proteins. European journal of pharmaceutics and biopharmaceutics...biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V, 130, ...

  41. Resume Writing for Non-academic Science Careers

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    Blog Post
    ...need to present yourself using a resume. Indeed, instead of having all the pages in a Curricula Vitae to...career, she transitioned into a communications role instead after she completed her MSc in Experimental Medicine...

  42. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ...vector developed by Feng Zhang’s lab at the Broad Institute (Sanjana et al., 2014). It is a dual-vector system...viruses are popular, pAAV-hSyn-DIO-hM4D(Gi)-mCherry contains a double floxed human muscarinic receptor 4 (Gαi-coupled...

  43. Building and Validating Recombinant Antibodies: A Case Study

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    Blog Post
    ... coexpression of light (green) and heavy (blue) chains as driven by two CMV promoters (orange). Figure... to WGS the lab’s entire hybridoma collection. Instead, the Trimmer Lab moved to direct functional validation...

  44. Sweating the Small Stuff: Details in the Lab

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    Blog Post
    ...for your workflow. Next time you make a mistake, instead of silently vowing never to do that again (I’ve...incubation periods to have another short "quiet period" instead of immediately running to the next thing.  It may...

  45. Antibodies 101: Buffers, Storage, and Conjugates

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    Blog Post
    ...buffer components could affect the reaction. For instance, sodium azide, a common antimicrobial agent added...relatively rare protein in an experiment with many proteins of interest! Research the brightness of your fluorophores...

  46. 7 Tips to Secure a STEAM Internship This Summer

    Type
    Blog Post
    ...communicates your interest in a specific program, and institution. Consequently it’s best to draft a cover letter...or mentor. Several days prior to the interview, inspect your technology, background, and lighting for a...

  47. New and Upcoming Viral Vectors - May 2020

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    Blog Post
    ...months: Lentiviral preps for expressing SARS-CoV-2 proteins ChR2 for targeted expression DREADDs GFP dependent... quickly shared plasmids expressing SARS-CoV-2 proteins at the beginning of April. Now, we've turned some...

  48. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    .... Projects such as the OpenFlexure Microscope (Collins, et. al., 2020), the miCube, SQUID (Hongquan, et...and low-cost optical setups. References Joel T. Collins, Joe Knapper, Julian Stirling, et. al, "Robotic...

  50. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...gene knockout in vivo and in vitro, as well as to insert targeted mutations through homologous recombination...effects and/or toxicity, it would be advantageous to instead use a preexisting, conserved cellular machinery...
Showing: 701 - 750 of 1040 results