We narrowed to 431 results for: %s

1. The Stingy Scientist: How the Baby Gel Box Was Born

   Type

   Blog Post

   ...need them.  When I was in graduate school in the 80’s, my lab was well funded and we didn’t feel the need...

2. Cre-Lox and Other Site-Specific Recombinases

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   Collection

   ...recombinases derived from P1 E. coli bacteriophage and S. cerevisiae , respectively. Other tyrosine recombinases...

3. Control AAV Preps

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   ... 5, 8, 9, rg*, PHP.eB Bryan Roth 51506 AAV phSyn1(S)-tdTomato-WPRE hSyn tdTomato Constitutive 5 Hongkui...

4. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

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   Blog Post

   ...PubMed PMID: 24727401. Bernstein, Jacob G., and Edward S. Boyden. "Optogenetic tools for analyzing the neural... PubMed Central PMCID: PMC3225502. Boyden, Edward S., et al. "Millisecond-timescale, genetically targeted...

5. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

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   Blog Post

   ...lower potential for off-target effects. In Gao et al.’s experiments, a single mismatch at any position of ...

6. Antibodies 101: Fc Effector Functions

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   Blog Post

   ...10.1038/cr.2009.139 Janda, A., Bowen, A., Greenspan, N. S., & Casadevall, A. (2016). Ig Constant Region Effects...

7. Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!

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   Blog Post

   ...134:1-17. PubMed PMID: 27984002. 2. Bilan, Dmitry S. et al. “HyPer-3: A Genetically Encoded H2O2 Probe...

8. New and Upcoming Viral Vectors - Spring 2019

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   Blog Post

   ... Plasmid Serotype Name 50363  AAV5  AAV phSyn1(S)-DreO-bGHpA 121675  AAV9  pAAV-EF1a-fDIO-Cre (FLP-dependent...

9. CRISPR 101: Drug discovery and CRISPR

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   Blog Post

   ...parental cell line and the X knockout with your drug(s). If a drug is equally toxic to both lines, it probably...

10. Plasmids 101: Gene, enhancer, and promoter trapping

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    Blog Post

    ...DOI: https://doi.org/10.7554/eLife.67667 Springer, S. P. Gene Traps. Plant Cell. (2000) 12(7): 1007-1020...

11. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

    Type

    Blog Post

    ...in the number of steps in the protocol. IgnaviCas9’s incredible thermostability makes the enzyme particularly...

12. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

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    Blog Post

    ...after infection with S. aureus (red). You can then see neutrophils swarming around S. aureus and eating ...

13. Antibodies 101: Designing Your First Flow Panel

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    Blog Post

    ...global excitation maximum lies around 650 nm, APC-Cy7's global excitation maximum is about 750 nm. If we were...fluorophores show efficient absorption at 650 nm, APC-Cy7's optimal excitation happens at higher wavelengths of...

14. In Vivo Biotinylation of Bacterial Fusion Proteins

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    Blog Post

    ... non-specific binding). Historically, glutathione-S-transferase (GST) fusion proteins have been immobilized...

15. Visualizing Translation at the Single Molecule Level

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    Blog Post

    ...molecule level. The coding sequence of Yan et al.’s reporter contains 24 copies of SunTag, a synthetic...

16. Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

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    Blog Post

    ...PI who generated the plasmid, followed by the PID(s) of your choice. Or if in doubt, copy and paste the...

17. The Power Behind NGS Plasmid Validation: seqWell

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    Blog Post

    ...tagging step. As an additional benefit of plexWell™’s sequential tagging procedure, the quantities of library...

18. Plasmids 101: Gram Negative and Positive Bacteria

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    Blog Post

    ...resources References Silhavy, TJ., Kahne, D. & Walker S. The Bacterial Cell Envelope. Cold Spring Harb Perspect...

19. Hot Plasmids and Viral Preps - September 2021

    Type

    Blog Post

    ...DNA repair.  A new CRISPR pooled library targeting S. pneumoniae D39V from Jan-Willem Veening is now available...

20. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

    Type

    Blog Post

    ...more robust set of frankenbodies. References Ewert, S., Honegger, A., & Pluckthun, A. (2004). Stability ...