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We narrowed to 459 results for: %s

Showing: 281 - 300 of 459 results
  1. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    ...involves making specific knock-out edits in a gene(s) of interest and has an average off-target frequency...CRISPR experiment? CRISPR 101: Cas9 The Other Cas(s) CRISPR 101: Validating Your Genome Edit         ...
  2. CRISPR Plasmids - Tagging

    Type
    Collection
    ...unavailable, you will need to design and clone in a gRNA(s) to guide the Cas9 protein to your target sequence...
  3. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...orthogonal nuclease-inactive Cas9's (dCas9) from S. pyogenes, N. meningitidis and S. thermophilus which have been...favorite cells. Ma et al., Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7. doi: 10.1073/pnas.1420024112...620nm) portions of the spectrum. PhD student Benjamin S. Padman recently developed a mitochondrially-targeted...context. Chatterjee A et al. Proc Natl Acad Sci U S A. 2013 Jul 16;110(29):11803-8. Plasmid tools for...
  4. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...topics. Resources and references References Aydin S (2015) A short history, principles, and types of ELISA...10.1016/0003-2697(81)90281-5 Pillai-Kastoori L, Heaton S, Shiflett SD, Roberts AC, Solache A, Schutz-Geschwender...
  5. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    .... Liu XS, Wu H, Ji X, Stelzer Y, Wu X, Czauderna S, Shu J, Dadon D, Young RA, Jaenisch R (2016) Editing...plasmids from this publication at Addgene. Stolzenburg S, Beltran AS, Swift-Scanlan T, Rivenbark AG, Rashwan...
  6. Antibodies 101: Validation

    Type
    Blog Post
    ...like Antibodypedia or through your antibody provider(s) if they accept and share user data on the antibody...10.1016/j.nbt.2018.08.002  Uhlen M, Bandrowski A, Carr S, Edwards A, Ellenberg J, Lundberg E, Rimm DL, Rodriguez...
  7. Hot Plasmids: February 2026

    Type
    Blog Post
    ...variants, resulting in mScarlet3-S2 (M163H/M66Q; the "S" is for "stable") (Ding et al., 2026). mScarlet3-S2...025-02962-2 Xiong, H., Chang, Q., Ding, J., Wang, S., Zhang, W., Li, Y., Wu, Y., … Fu, Z. (2025). A highly...
  8. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...Hiraizumi, M., … Abudayyeh, O. O., & Gootenberg, J. S. (2025). Reprogramming site-specific retrotransposon...Sanchez, A. D., Udeshi, N. D., Svinkina, T., Carr, S. A., Feldman, J. L., Perrimon, N., & Ting, A. Y. (...
  9. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...lately (1, 2), depends on knowledge of the structure(s) a given phage will recognize on a host cell. To ..., Callum J., Mohammadali Khan Mirzaei, and Anders S. Nilsson. "Adapting drug approval pathways for bacteriophage-based...
  10. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...as effective at editing mouse embryos as Cas9 from S. pyogenes and has the advantage of being smaller (...health.     References Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini...
  11. Control AAV Preps

    Type
    Collection
    ... 5, 8, 9, rg*, PHP.eB Bryan Roth 51506 AAV phSyn1(S)-tdTomato-WPRE hSyn tdTomato Constitutive 5 Hongkui...
Showing: 281 - 300 of 459 results