We narrowed to 700 results for: BASE;

...introduce insertions, deletions, and all possible base-to-base changes.   1. Plasmids 101: Transformation,...

...know it’s there? For this task, you can use PCR based detection, DNA staining, fluorescent tagging, agar...and cost-effective (Sung, 2006). The sensor cell-based method uses specialized cells that can detect the...from different biotech companies which are enzyme-based mycoplasma detection kits. These kits detect mycoplasma...YJ, Hong JT, Chung YB, Lee CK, Song S (2006) PCR-based detection of mycoplasma species. J Microbiol .44...

... in this blog post, we're going to focus on PCR-based applications (qPCR and ddPCR.) qPCR Quantitative.... Quantitative analysis of copy number variants based on real-time LightCycler PCR. Curr Protoc Hum Genet...genetic elements can be determined by SYBR-Green-based quantitative real-time PCR. J Microbiol Methods....sample with known copy number set as the expression baseline. Copy number is then determined by comparing results...

...lab developed a polymerase chain reaction (PCR)-based system that allows users to generate a plasmid containing...Masato Kanemaki's lab has developed a CRISPR/Cas-based system for tagging endogenous proteins with an auxin-inducible...Schmid-Burgk, Jonathan L., et al. “CRISPaint allows modular base-specific gene tagging using a ligase-4-dependent...

...experiences with the new method and look for advice/tips. Based on the discussions in the forums, it appeared that...majority of the respondents (70%) used a lipofectamine-based method to deliver NgAgo ingredients into the cell.... Overall, the real take-home message thus far, based on survey respondents "Final Verdict", is that more...

...microscope with labels. Created with BioRender.com. Base Light Source Condenser and Diaphragm Stage Objective...carry it with two hands - one hand supporting the base and the other holding the arm - or use a cart. Revolve...

...in order to increase the accessibility of CRISPR-based phenotypic screen datasets. Original published CRISPR...demonstrated the feasibility and power of CRISPR-based library screens in cell lines from humans and other...? The Open Repository of CRISPR Screens (ORCS) database is a new freely available resource developed by...which can also be used to search the entire CRISPR database for particular screens of interest.   Figure...screen data included in the BioGRID ORCS CRISPR database are encouraged to contact BioGRID curators at ...

..., as it only changes one gene at a time. CRISPR-based systems enable gene knockout in immune cells in ...out a single gene in defined cell types (lineage-based) pXPR_070 Violet-excited GFP (vex) Lineage-Cre...

... inspired to produce intrachromosomal deletions based on the experiments of Kim and colleagues using zinc.... Regulatory DNA function is typically inferred based on correlative biochemical characteristics and ectopic...

...dedicated to generating new tools to manipulate biology based on molecular features found in nature, with the ...recently introduced the SpyTag/SpyCatcher system, based on a protein isolated from Streptococcus pyogenes...

...providing a platform for the distribution of plasmid-based tools that are useful for COVID-19 ...

...would represent a major step forward for CRISPR-based RNA editing - and further experiments strongly supported...CRISPR could cut DNA, the second generation could do base editing, and with the third generation came Prime...ability to now make any small changes, including base edits, insertions, and deletions. But CRISPR’s uses...deletions and small insertions, leaving a several kilobase-sized gap in the gene editing field. The AbuGoot...now developed PASTE, which can “drag and drop” kilobases’ worth of DNA with CRISPR-driven precision into...

...label based on antibody-like sensors) changes that. Developed by the Arnold Lab, ATLAS is based on an ...synapses, and cytoskeleton components) and in a FRET-based biosensor for cAMP dynamics. The mGold2 variants...

...the most popular options, so most of this post is based on reading results from a NanoDrop. The principles...calculate a sample’s nucleic acid concentration based on the sample’s absorption at 260 nm (A260). ...use a complementary assay, such as a fluorescence based assay or running an agarose gel. In the case of ...

...number of ways, from small edits that change single base pairs, to chromosomal inversions and large deletions...1 CRISPR systems make up the majority of CRISPR-based immune systems in bacteria and archaea. Class 1 ...deletions of up to hundreds or even thousands of base pairs, stopping either at boundaries defined by ...originally suspected that Cas3 moved a couple of kilobases down the strand of DNA, Cascade caught up to it...

..., technical support is often covered by the lab based team as part of their general job duties. Bigger...etc. Technical support roles are primarily office based, and email/phone are the most popular ways to communicate... pressure which are often found in sales or lab-based roles in industry. What to look forward to in a ...answers for customers, internal resources and databases are scrutinized, and this information is enriched...

... the very beginning of the third chromosome, ten base pairs in length. An emerging family of sequencing...increase the expression of a gene that is millions of base pairs away. (For details on DNA compaction and the...parsed for spCas9 PAM sites (NGG) and then filtered based on two main criteria: no TTTTT allowed (this is ...here with permission. Quilt is a collaborative database for genomics. In this article, Quilt CTO Aneesh...is CTO at Quilt Data. Quilt is a collaborative database. Aneesh's research interests span machine learning...

...common to use many preys with a single bait in Y2H-based screens. In this way, proteins that interact with...proteome of a particular cell type, or a subset based on a cellular compartment or growth stage). A prey...protein-protein interactions in mammalian cells based on protein splicing. Anal Chem. 2001; 73:2516-2521... Including a prey-only control also provides a baseline level of reporter activation. Vary the expression...

...immunoglobulin genes, recombinant antibodies are plasmid-based. Their sequences can be optimized for specificity...Arbabi-Ghahroudi et al., 2017). Although they’re based on the immune system of llamas, synthetic nanobodies...mixture of proteins. The proteins are separated based on molecular weight by SDS-PAGE, and are subsequently...well as tools for developing and producing plasmid-based antibodies - and we’re always growing our collection...

...Thinking about using antibody-based applications in your experiment...but not sure where to start or ...