...Conditional (Cre-lox), stable expression of shRNAs; addition of Cre turns on shRNA expression. See article...Envelope VSV-G-expressing envelope plasmid Trono 8454 pCMV-VSV-G N/A Envelope VSV-G-expressing envelope plasmid...pCPRDEnv 2nd Packaging part of the FELIX vector system, expresses Gag-Pol and Rev Nolan 35617 pCAG-Eco N...MLV expressing envelope plasmid Nienhuis and Salmon 35616 pCAG-VSVG N/A Envelope VSV-G-expressing envelope...article for more cre-lox shRNA expression variants. Jacks 19319 pLJM1-EGFP 3rd CMV-driven EGFP fusion; can...constitutive transgene expression, contains intron that gives you high expression. Trono 12254 pWPI 2nd... plasmid for cDNA expression Ramalho-Santos 24129 pULTRA 3rd bi-cistronic expression of EGFP and the gene...

... whatever molecule you choose (Figure 1). Just express the fusion protein and add a specific ligand! A...better detect dim structures or low levels of expression with the same imaging workflows you already use...to drive cell type-specific delivery of a viral vector (Strebinger et al., 2023). All this flexibility...no wonder that self-labeling protein tags are increasingly popular. Embrace the flexibility and switch ...

...Microbiology Plant Expression Stem Cells Synthetic Biology Tet Inducible Expression Worm Expression Kits Kits ... Fluorescent Proteins Protein Expression Synthetic Biology Viral Vectors Communities Find a scientific...Genome Engineering Genome Engineering Overview CRISPR Cre-lox TALENs Zinc Fingers Luminescence Fluorescent ... Topics Antibody Plasmid Collection Bacterial Expression Systems Empty Backbones Genetic Code Expansion...Genome Engineering EXtracellular Plasmid RESource (EXPRESs) Consortium Krüppel-like factors (KLF) Plasmid ...

... Flp-dependent Cre and Flp-dependent Cre, Flp, and VCre-dependent Constitutive (non-cre-dependent) Clear... Viral Vector Packaging Service AAV Controls Viral Vector Packaging Service: Control...None Other Cre-dependent color switch Brainbow constructs Spaghetti monster tags Activity Cre-dependent...EF1a EYFP Cre dependent 1, 2, 5, 9, rg* Deisseroth 28306 pAAV-FLEX-tdTomato CAG tdTomato Cre dependent...nls-EGFP Cre dependent 1, 2, 5, 8, 9, rg* Harvey 131002 pAAV-Ef1a-DIO mScarlet EF1a mScarlet Cre dependent... hSyn EGFP Cre dependent 1, 2, 5, 8, rg* Roth 50459 pAAV-hSyn-DIO-mCherry hSyn mCherry Cre dependent 1...pCAG-FLEX-EGFP-WPRE CAG EGFP Cre dependent 1, 2, 5, 8, 9, rg* Zeng 59331 pAAV-CAG-FLEX-EGFP CAG EGFP Cre dependent 1 Wickersham...

...targeting vector assembly, and the remaining 5 plasmids include Cre and FLP expression vectors, a general... one vector from the Doyon lab, eSpCas9(1.1)_No_FLAG_AAVS1_T2 (Addgene #79888) , which expresses an untagged...gene-specific CRISPR and donor vectors as well as pX330S-2-PITCh, expressing a generic gRNA targeting the...Plasmids: pX330S-2-PITCh - expresses Cas9 & PITCh-gRNA pX330A-FBL/PITCh - expresses Cas9, PITCh-gRNA, & FBL-specific...are available. An improved FLP recombinase expression vector allows for efficient marker cassette FLP-out...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...has been adapted to allow tagging of proteins expressed from their natural chromosomal context. These ...

...cleave DNA and instead activates/represses gene expression by various mechanisms. The RNA guide is typically...- GUIDE-Seq and integrase-defective lentiviral vectors - which rely on insertion of DNA by the NHEJ pathway...differences. If you are performing CRISPR library screens, the same principle holds true - the higher the...lower the likelihood of an off-target artifact. Increasing the N in your experiment is the only way to effectively...effectively control for off-targets and significantly decrease the probability of artifacts. Off-target controls...Rabinowitz R, Offen D. Single-Base Resolution: Increasing the Specificity of the CRISPR-Cas System in Gene...

.../Gaussia Creation of C-terminal Red Luciferase fusion for expression in plants, co-expressed with Gaussia...regions Joshua Mendell 212936 pGL3 Basic Vector Firefly Vector for investigating regions controlling transcription...C-terminal Firefly luciferase fusion for expression in plants, co-expressed with mScarlet-I for ratiometric imaging...as a reference level of expression. Use this table to find plasmids expressing luciferase. We also offer...Retroviral expression of firefly luciferase Scott Lowe 14080 pAKlux2 Lux Operon Bacterial expression of luciferase...Mammalian expression of Nano-lantern Takeharu Nagai 87121 pcDNA-RLuc8 Renilla CMV Mammalian expression renilla...CMV AAV expression of firefly luciferase and GFP James Wilson 22522 phGluc Gaussia EF1α Expression of Gaussia...

...MK, Elfinger M, Hartl D, Rudolph C. Transgene expression of transfected supercoiled plasmid DNA concatemers... JA, Carnes AE, Hodgson CP. Plasmid DNA vaccine vector design: impact on efficacy, safety and upstream...generated by SnapGene software.  Thanks to increasing affordability and accessibility, more researchers...by growing seed stocks at 30°C compared to 42°C. Screen multiple colonies to isolate the monomer Sometimes... 1984; Summers et al., 1993). In these cases, screening multiple bacterial colonies using one of the detection...DNA-forming sequences and WRN deficiency independently increase the frequency of base substitution in human cells... self-control and a sense of direction are the secrets of multicopy plasmid stability. Mol Microbiol. ...

...cloning vector Retroviral vectors: neomycin (pSIR-neo) , GFP (pSIR-GFP) , DsRed (pSIR-DsRed-Express2) , human... sgRNA dual expression: pAC2 , pAC152 , pAC153 , pAC154 ; pmax dCas9 activator expression: pAC91 , pAC92... pooled library amplification 1 vector system: lentiCRISPR v2 2 vector system: lentiCas9-Blast and lentiGuide-Puro...protocol Church gRNA design and cloning gRNA cloning vector PDF 115.2 KB Church gRNA design and cloning for...pCFJ90 (pharyngeal co-injection marker); pDD104 (Cre recombinase) PDF 102.5 KB Jaenisch CRISPR plasmid...human pluripotent stem cells pCas9_GFP ; gRNA empty vector Link O'Connor-Giles Fly: gRNA and ssODNs design...pX330) Link Parrott NEBuilder Assembly of CRISPR vectors using ssDNA oligos p201G Cas9 ; p201B Cas9 ; p201H...

...for many molecular biology protocols, including vector-insert joining for recombinant plasmid construction..."Cloning, nucleotide sequence, and engineered expression of Thermus thermophilus DNA ligase, a homolog...reliably detect single-base differences. The original creators of this technique visualized the ligation product...quencher pairs or qPCR-based methods to greatly increase the sensitivity of detection (14-20). LDR has ... 27). This methodology has been used to rapidly screen buffer conditions for Taq DNA Ligase and their ...electrophoresis (CE) (see below). This method allowed screening of all possible base combinations (Watson-Crick...of a 96-well plate, allowing 6 conditions to be screened per plate. The results indicated that the optimal...

...inducible vector . Harley et al. Cell Rep. 2023 Dec 26. Induce CNS cells with concomitant expression of aggregation-prone...Noteworthy: AAV.rTH.PI.Cre.SV40 ready-to-use viral prep useful for dopamine research. CBA-driven, Cre-dependent...Neurodegeneration Plasmid Collection A collection of plasmids expressing wild type or mutant ORFs, gene fragments, and...to neurodegenerative disorders. Find plasmids expressing HTT, C9ORF72, LRRK2, Synuclein, and more. New...Cre-dependent expression of diphtheria toxin receptor (DTR)–GFP fusion protein. Azim et al. Nature. 2014 Apr 17...Dec 2. Use PiggyBac plasmids with tet-inducible expression of transcription factors for sensory, motor, ...with rapid delivery. Includes antibodies, viral vectors, animal models, purified protein, and more. Plasmids...

...cell type-specific expression. The Allen Institute for Brain Sciences has screened thousands of enhancers...Isocortex 230803 pAAV-AiE2638m-minBG-iCre(R297T)-BGHpA AiP20142 AiE2638m Cre Layer 2-3_IT Isocortex 214583 pAAV-AiE2543m-minBG-SYFP2...Isocortex 220727 pAAV-AiE2543m-minBG-iCre(R297T)-BGHpA AiP2048 AiE2543m Cre Layer 2-3_IT Isocortex 224004 pAAV-AiE0680m-minBG-SYFP2...Isocortex 230402 pAAV-AiE0680m-minBG-iCre(R297T)-BGHpA AiP15072 AiE0680m Cre Layer 2-3_IT Isocortex 223974 pAAV-AiE0050m...224056 pAAV-AiE0050m_3xC2-minBG-iCre(R297T)-BGHpA AiP13152 AiE0050m_3xC2 Cre Layer 4_IT Isocortex 224038 ...220734 pAAV-AiE0671m_3xC2-minBG-iCre(R297T)-BGHpA AiP20026 AiE0671m_3xC2 Cre Layer 4_IT Isocortex 224043 ...220735 pAAV-AiE2182m_3xC2-minBG-iCre(R297T)-BGHpA AiP20027 AiE2182m_3xC2 Cre Layer 5_IT Isocortex 214522 ...

...element Activity Cre-dependent Flp-dependent Cre and Flp-dependent Cre, Flp, and VCre-dependent Constitutive... Viral Vector Packaging Service AAV Biosensors Viral Vector Packaging Service: Biosensor...none Cre dependent 1, 5, 9, rg* GENIE 162382 pGP-AAV-CAG-FLEX-jGCaMP8f-WPRE CAG jGCaMP8f none Cre dependent... none Cre dependent 1, 9, rg* GENIE 162380 pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE CAG jGCaMP8s none Cre dependent... none Cre dependent 1, 9, rg* GENIE 162381 pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE CAG jGCaMP8m none Cre-dependent...none Cre dependent 1, rg*, PHPeB Kim 104495 pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE CAG jGCaMP7s none Cre dependent... none Cre dependent 1, 9 Kim , GENIE 100839 pAAV.CAG.Flex.GCaMP6m.WPRE.SV40 CAG GCaMP6m none Cre dependent...

... FRET Patrick Daugherty FRET: Vector for arabinose inducible expression of an N-terminally His-tagged ...David Waugh Empty lactose/IPTG inducible vector for expression of a recombinant protein with an N-terminal...site to remove tag Nicola Burgess Brown pGEX expression vector with N-terminal GST tag and TEV cleavage site... 6x-His tag Ophear Gileadi pET-28-a based expression vector containing N-terminal, TEV cleavable, 6x-His...Arrowsmith Empty lactose/IPTG inducible vector for high expression of a recombinant protein with a 6x His...

...UTRs are included in the vector to enhance expression of the message. Both vectors utilize homodimeric FokI...contains modified pFUS array vectors, destination vectors and a reporter vector for mammalian cell-based ...Destination vectors, pcDNA-TAL-NC2 and pCAGGS-TAL-NC2, are mammalian expression/mRNA synthesis vectors containing...much higher than the parental vectors. pC-GoldyTALEN directs expression of TALENs from a truncated CAGs...placing TALORs built into this vector under galactose-inducible expression. pTAL6-BB contains the TEF1 promoter...promoter, giving constitutive expression of TALORs built into this vector. 36034 pTAL6-BB Golden Gate TALEN...1214–2210 of pTAL3 were cloned into a pCS2 expression vector resulting in shorter N- and C-terminal tal...

...subsequent increase in expression by the activation domain, resulting in reduced gene expression. This idea...Tetracycline (Tet) Inducible Expression Tetracycline (Tet) Inducible Expression Additional Resources: Browse... rtTA or TetR expression plasmid Stable cell lines can be made that continuously express a system component...Wiederschain 41393 pCW57.1 Lentiviral vector for inducible expression for Gateway cloning; selection cassette...Root 11651 pLVUT-tTR-KRAB Lentiviral vector for inducible expression of transgene or shRNA; see article ...Vogelstein 25735 pSLIK-Neo Lentiviral vector for inducible expression of a miR-shRNA; selection cassette ...On Fraser 44012 pInducer20 Lentiviral vector for shRNA expression; see article for additional tookit plasmids...

...Mammalian Expression HcRed1-N1 - Mammalian Expression HcRed1-C1 - Mammalian Expression dKatushka 588 635 22...pBad-EBFP2 - Bacterial Expression EBFP2-N1 - Mammalian Expression EBFP2-C1 - Mammalian Expression EBFP2-pBAD - ...- Mammalian Expression pCyPet-C1 - Mammalian Expression pCyPet-His - Bacterial Expression CYPet-N1 - Mammalian...pEiLOV-N1 - Mammalian Expression pGEX iLOV - Bacterial Expression iLOV-N1 - Mammalian Expression iLOV-C1 - Mammalian...Mammalian Expression pCAG-GFP - Mammalian Expression pLV-eGFP - Mammalian Lentiviral Expression Ac5-STABLE1...1 - Mammalian Expression mEGFP-N1 - Mammalian Expression mEGFP-C1 - Mammalian Expression mEGFP-pBAD - ... - Mammalian Expression (this is a bicistronic vector rather than a fusion protein vector) ZsGreen1-N1...

... a single mammalian expression vector A tool kit for rapid cloning and expression of recombinant antibodies...Aug 28;242(1-2):159-81. Carlos Barbas Vector system for expression, dimerisation and detection of recombinant.... Gavin Wright pET-30-based vector dedicated to efficient scFv expression, which circumvents the problem...problem of in-frame amber codons in scFvs A new expression vector facilitating production and functional analysis...recombinant antibodies, created by cloning antibody components into plasmids and expressing these in bacteria...plasmid collections that can be used to create and express plasmid-based antibodies. Description Article... trusted partner labs. Learn More Cloning and Expressing Antibodies Antibody Plasmids Antibodies are large...

...designed for creating plant expression vectors: A modular plasmid assembly kit for multigene expression, gene...Parniske. GreenGate Cloning System to create plant expression vectors containing several cassettes and generate...maize and Arabidopsis) and vectors for easy assembly of one or more gRNA expression cassettes - Qi-Jun Chen...microRNA and siRNA vectors in Arabidopsis - James Carrington. JoinTRV , an improved vector system based on... - Tools for gene and protein expression and mapping of gene expression in plants. Figshare - Plant Illustrations... that drive general and species-specific gene expression (see the column ‘promoter’ in the tables for ...CRISPR Resources for a full selection of plasmids expressing different functional variants of Cas9 and empty...

...Consortium: Nuclease Expression Vectors This page lists plasmid vectors designed to express nucleases in different...pST1374 (for expression in mammalian cells or zebrafish) pDW1775 (for expression in plants) Vectors encoding...This page lists plasmid vectors designed to express nucleases in different cell types. Zinc finger arrays... Plasmid Collections Zinc Finger Consortium Nuclease Expression Vectors Zinc Finger...transferred in-frame to any of these vectors using unique cloning sites. Vectors encoding wild-type FokI nuclease...in-frame to these vectors. Plasmid...heterodimeric FokI nuclease domains: pMLM290 (for expression of a “+” heterodimeric FokI domain. Miller et...