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  1. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    ...ability to infect cells with a high efficiency and deposit a DNA/RNA cargo into their targets makes them attractive...Vector Pages Learn About Viral Vector Biosafety Deposit Your Viral Vectors ...

  5. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    ...Ramanathan et al. also used the Contamination Repository for Affinity Purification (CRAPome), to identify...identify highly biotinylated background proteins reported by other research groups that performed BirA*-based...

  6. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    ...catalog would allow us to enhance our biosensor repository as we find ourselves lacking several crucial ...open science, Hébert plans on building upon and depositing their modified constructs to Addgene. Congratulations...

  8. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    ... be noted that the photostability measurements reported in the literature are performed in different ways...information can help guide your understanding of reported data. Photobleaching results in a loss of fluorescence...

  12. Working with Nuclear Receptors

    Type
    Blog Post
    ...the development of a vast number expression and reporter plasmids and the free exchange of these vectors...characterization of NRs and coregulators, and although the reporter gene has over the years evolved from chloramphenicol...

  15. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ...such as the serotonin 3 receptor, will promote depolarization in neurons. Alternatively, combining with the...DREADD-based chemogenetics in mice. Scientific Reports 9: https://doi.org/10.1038/s41598-019-41088-2 Magnus...

  16. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ...they are integrating viruses – they physically deposit their cargo into the host’s DNA. These viruses ...familiarize yourself with your organization’s reporting mechanisms before you start an experiment, just...

  18. Tips for Technical Support Calls

    Type
    Blog Post
    ...the experiments performed by our thousands of depositors. That means their original articles are your ... Addgene Help Center Get Help with the Addgene Deposit Process Resources on Addgene.org Check Out Our...

  19. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    ...in mammalian cells, LbCpf1 and AsCpf1. In both reports, on target editing efficiency for the Cpf1 orthologs...mismatches, but variably so, with Kleinstiver et al. reporting that Cpf1 can tolerate mismatches at gRNA positions...
Showing: 281 - 300 of 648 results