We narrowed to 658 results for: EPO;
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TypeBlog Post...off for the day. 1:45 PM--Write final report. Work on a final report for a user research project I led. ...
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Building and Validating Recombinant Antibodies: A Case Study
TypeBlog Post...antibodies expressed from plasmids that have been deposited with Addgene. Here, we take a closer look at the... VH and VL sequence. These plasmids have been deposited with Addgene for distribution to the scientific... -
Will You Be My Mentor? Finding and Asking for Mentoring Support
TypeBlog Post... get started with mentoring. Read this Catalyst Report “Making Mentoring Work” to learn about what makes...make your mentor want to keep working with you is report back with progress. Tell them how their advice ... -
Choosing the B(right)est Fluorescent Protein: Photostability
TypeBlog Post... be noted that the photostability measurements reported in the literature are performed in different ways...information can help guide your understanding of reported data. Photobleaching results in a loss of fluorescence... -
RaPID Detection of RNA-protein Interactions
TypeBlog Post...Ramanathan et al. also used the Contamination Repository for Affinity Purification (CRAPome), to identify...identify highly biotinylated background proteins reported by other research groups that performed BirA*-based... -
It’s Bioelectric! An Exciting Interdisciplinary Field of Research
TypeBlog Post...differentiated and quiescent it is, and the more depolarized (positive) the cell is, the more embryonic, stem...technologies are now available for fluorescent reporting and genetically encoded fluorescent voltage indicators... -
Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards
TypeBlog Post...research. Abhi Aggarwal: Glutamate sensing reporters Abhi Aggarwal is a postbaccalaureate research ...third-generation intensiometric glutamate sensing fluorescent reporter (iGluSnFR3) to study glutamate, a signaling molecule... -
Supporting Rare Disease Research with the 2020 BeHEARD Awards
TypeBlog Post...catalog would allow us to enhance our biosensor repository as we find ourselves lacking several crucial ...open science, Hébert plans on building upon and depositing their modified constructs to Addgene. Congratulations... -
The Future of Research Symposium Boston 2015
TypeBlog Post...workforce working group report. http://acd.od.nih.gov/biomedical_research_wgreport.pdf Resources at Addgene... -
CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt
TypeBlog Post...are easy with CRISPR. Addgene: You’ve made many deposits to Addgene over the years. Can you describe the...will be available on GitHub, an open source code repository. Addgene: Over the longer term, where is this... -
Working with Nuclear Receptors
TypeBlog Post...the development of a vast number expression and reporter plasmids and the free exchange of these vectors...characterization of NRs and coregulators, and although the reporter gene has over the years evolved from chloramphenicol... -
Plasmids 101: The Wide World of Natural Plasmids
TypeBlog Post...researchers to develop and deposit the many plasmid tools available in the Addgene repository. To learn more about... -
Pathways Over Time Plasmids Engage Students in Functional Genomics Research
TypeBlog Post...Associate Professor at Boston College. National reports stress the importance of providing authentic research...Students have used these plasmids, which have been deposited with Addgene, to successfully analyze Met protein... -
Viral Vectors 101: Chemogenetics
TypeBlog Post...such as the serotonin 3 receptor, will promote depolarization in neurons. Alternatively, combining with the...DREADD-based chemogenetics in mice. Scientific Reports 9: https://doi.org/10.1038/s41598-019-41088-2 Magnus... -
Viral Vectors 101: Virus Safety
TypeBlog Post...they are integrating viruses – they physically deposit their cargo into the host’s DNA. These viruses ...familiarize yourself with your organization’s reporting mechanisms before you start an experiment, just... -
Split Fluorescent Proteins for Studying Protein-Protein Interactions
TypeBlog Post... (abundant in eukaryotes) A Near-Infrared BiFC Reporter for In Vivo Imaging of Protein-Protein Interactions...green-yellow or orange-red fluorogen A split fluorescent reporter with rapid and reversible complementation. Tebo... -
Tips for Technical Support Calls
TypeBlog Post...the experiments performed by our thousands of depositors. That means their original articles are your ... Addgene Help Center Get Help with the Addgene Deposit Process Resources on Addgene.org Check Out Our... -
Cpf1 Update: Comparison to Cas9 and NgAgo
TypeBlog Post...in mammalian cells, LbCpf1 and AsCpf1. In both reports, on target editing efficiency for the Cpf1 orthologs...mismatches, but variably so, with Kleinstiver et al. reporting that Cpf1 can tolerate mismatches at gRNA positions... -
7 Simple Strategies to Resolve Conflicts with Difficult Supervisors
TypeBlog Post...supervisor might expect weekly or biweekly progress reports. However, a hands-off busy supervisor might get...update, and whether he or she would like a written report or to meet in person. Regardless of your supervisor... -
PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs
TypeBlog Post... In our study, we used the TRIP (Thousands of Reporters Integrated in Parallel; Akhtar et al., 2013; Schep...Chromatin Position Effects Assayed by Thousands of Reporters Integrated in Parallel. Cell, 154(4), 914–927....