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  1. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    ...request to any of these people, including and especially the hiring manager. This is considered intrusive...own good ideas, events and favorite resources. Respect the network and it will reward you. Resources ...

  2. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    ...abundance of a particular barcode sequence then corresponds to the abundance of a specific clone; lineage... C. Bhang, et al. "Studying clonal dynamics in response to cancer therapy using high-complexity barcoding...

  4. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    ...successful outcome and the good ones take this responsibility seriously. As I make my rounds speaking at ...Schachter Consulting is part of that group and she is especially excellent in coaching for communication skills...

  8. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ..., some adenovirus strains are known to cause respiratory diseases in humans, earning this virus a BSL-... and closely follow safety protocols. This is especially true when you’re tired, in a rush, or having ...

  9. Design Tips for Prime Editing

    Type
    Blog Post
    ...great tips to guide your experimental design, especially the design of the RNA sequences you’ll use in...increase the odds of incorrect editing outcomes, especially when MMR is inhibited. Created with BioRender.com...

  10. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    ... from the membrane along with the antibodies, especially with more stringent stripping methods. If you...horseradish peroxidase (HRP) or Alexa Fluor dyes, respectively. Chromogenic detection produces colored precipitates...

  13. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    ...trait to their granddaughters that reduced their lifespan. Because these dramatic conditions were experienced...so the syndromes are caused by a lack of the corresponding gene products due to the same region, but not...

  14. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    ...have to, but this is tough for women in skirts, especially short people – which I am, so I hate stools)....listing some and asking people to raise hands in response. I am always ready with some written poll questions...

  16. Academic vs. Industry Postdocs

    Type
    Blog Post
    ...Academic postdocs can be long—5 years or more, especially in the biological sciences—whereas industry postdocs...make that transition from an academic setting—especially if job applications require teaching experience...

  17. Hot Plasmids and Viral Preps - May 2021

    Type
    Blog Post
    ...deployed around the world to combat severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus...cell-surface receptor. The spike protein RBD responsible for binding ACE2 is the target of most anti-SARS-CoV...

  18. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    ...during the production process? These questions are especially important when producing multiple vectors in ... with the most total reads are most likely to correspond to the contaminant. Each contig is then BLASTed...

  19. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ...complementary and non-complementary to the gRNA, respectively. When complexed with a truncated gRNA, Cas9 ...transcriptional activation or repression at the respective sites.   Many thanks to our guest bloggers Alissa...

  20. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ... agitated with sudden changes in temperature (especially if you have air conditioning on) so leave them...cell line you’re using and its passage number. Especially in a lab where multiple people are working on...
Showing: 281 - 300 of 628 results