We narrowed to 639 results for: ESP
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TypeBlog Post...request to any of these people, including and especially the hiring manager. This is considered intrusive...own good ideas, events and favorite resources. Respect the network and it will reward you. Resources ...
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Addgene's Tips for Plasmid Quality Control
TypeBlog Post... reverse reactions, respectively, and then shipped along with their corresponding glycerol plate on dry... -
New Tool for Lineage Tracing: The ClonTracer Library
TypeBlog Post...abundance of a particular barcode sequence then corresponds to the abundance of a specific clone; lineage... C. Bhang, et al. "Studying clonal dynamics in response to cancer therapy using high-complexity barcoding... -
CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt
TypeBlog Post...twice. The second problem was the shape of the response function or how the output changes as a function...turns on or off. CRISPR shows a more continuous response - not switch-like at all. It’s also difficult ... -
Career Coaching for Scientists: Why and Where Do I Find One?
TypeBlog Post...successful outcome and the good ones take this responsibility seriously. As I make my rounds speaking at ...Schachter Consulting is part of that group and she is especially excellent in coaching for communication skills... -
Viral Vectors 101: Virus Safety
TypeBlog Post..., some adenovirus strains are known to cause respiratory diseases in humans, earning this virus a BSL-... and closely follow safety protocols. This is especially true when you’re tired, in a rush, or having ... -
Design Tips for Prime Editing
TypeBlog Post...great tips to guide your experimental design, especially the design of the RNA sequences you’ll use in...increase the odds of incorrect editing outcomes, especially when MMR is inhibited. Created with BioRender.com... -
Antibodies 101: Stripping and Reprobing Western Blots
TypeBlog Post... from the membrane along with the antibodies, especially with more stringent stripping methods. If you...horseradish peroxidase (HRP) or Alexa Fluor dyes, respectively. Chromogenic detection produces colored precipitates... -
Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation
TypeBlog Post...Recently, however, a particular tool has gained widespread popularity – and notoriety – as a tool of choice...should work well, and up to 10 base pairs away (especially 3’ of PAM) might work too. High dCas9 and gRNA... -
A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells
TypeBlog Post...can be challenging to make these base changes, especially in primary human cells. Now, scientists at Beam... 3 genes (98.1%, 98.3%, or 98.6% efficiency, respectively) and outperformed ABE7.10 by at least 1.4 fold... -
Synthetic promoter AAVs for cell-type specific expression in retinal cells
TypeBlog Post...they are costly and time-consuming to generate, especially for large animals such as primates. Viral vectors...Morikawa, R.K., Rompani, S.B., Hantz, P., Szikra, T., Esposti, F., Cowan, C.S., Bharioke, A., Patino-Alvarez,... -
Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells
TypeBlog Post...pluripotency marker expression and response to LPS stimulation, respectively. Primary cell type Gene targeted... -
Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015
TypeBlog Post...experience helping scientists share plasmids, especially those of the CRISPR variety (see slides from ...conserved in mammals, expressed in neurons, and are especially abundant in neuronal synapses. shRNA silencing... -
Illuminating Epigenetics with A FRET Based Biosensor
TypeBlog Post...trait to their granddaughters that reduced their lifespan. Because these dramatic conditions were experienced...so the syndromes are caused by a lack of the corresponding gene products due to the same region, but not... -
How-to: 5 Steps to a Great Panel Discussion
TypeBlog Post...have to, but this is tough for women in skirts, especially short people – which I am, so I hate stools)....listing some and asking people to raise hands in response. I am always ready with some written poll questions... -
Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)
TypeBlog Post... a company. Revolution Bioengineering is two responsible adults in their 30s who have quit their academic...development: building a plant that could sense and respond to novel substances. We both had an applied science... -
Of Myc and Men
TypeBlog Post...interest to better study them in vivo and in vitro, especially in the mid-late 20th century with the advent ...tumorigenesis. Using two different synthetic peptides corresponding to linear sequences within the middle of the... -
PhD Applications After COVID
TypeBlog Post...so reach out to three in case one isn’t able to respond to your email. During my PhD application cycle,...interview from a travel day to an online call in response to challenges on their end. Though the quick changes... -
Hot Plasmids and Viral Preps - May 2021
TypeBlog Post...deployed around the world to combat severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the virus...cell-surface receptor. The spike protein RBD responsible for binding ACE2 is the target of most anti-SARS-CoV... -
10 Basic tips for mammalian cell culture
TypeBlog Post... agitated with sudden changes in temperature (especially if you have air conditioning on) so leave them...cell line you’re using and its passage number. Especially in a lab where multiple people are working on...