...optogenetic reporter, channelrhodopsin, fused to a fluorescent GFP marker (CatCH-GFP). The lab deposited AAV...promoter to drive expression of a Cre-dependent fluorescent CatCH-GFP reporter. Expression of GFP will only...

...that the method of Cas9 and gRNA delivery can influence the level of off-target effects, with electroporation...change to large insertions like the addition of a fluorophore or tag. To utilize HDR, a donor template containing...

...for monitoring homemade microfluidic chips using the fantastic optics and fluidics platform it contains....

...for visualization of proteins of interest. A fluorescent Halo ligand can be added to media, buffer, etc...there are antibodies to it and ways to achieve fluorescence with it. AID The auxin-inducible degron (AID...

... more than 80-85% confluent because cells like their space. If they reach confluence, they stop growing...

...types used for packaging viral particles (6), can influence vector performance. Open science initiatives, ...how small changes in vector administration can influence AAV expression properties.  By providing many ...

...Cas9, such as visualizing genomic loci using fluorophore tagged dCas9 may require the use of plasmid or...is required, such as when a Cas9 fusion to a fluorescent protein is used to label nucleic acid targets...

... pL2_pSB90_2x35S::fLUC-I::tNOS & pSB166 - pL2_pSB90_tMAS::rLUC-I::pMAS. Fluorescent proteins (FP, e.g....

... manipulated by injected RNA and can express fluorescent proteins. This lab has created an automated tank... process is expensive. They are using a cool fluorescent indicator system to rapidly identify recombinants...

...tissues such as blood cells or neurons can be fluorescently labeled, allowing researchers to observe changes...about zebrafish behavioral studies Download the fluorescent proteins 101 eBook Read our plasmids 101 blog...

...overlapping AAV since both of these variables influence whether cells will be co-transduced with multiple...Drawbacks: The choice of homology sequence can influence the level of transgene expression, and may need...

...; Cook et al., 2020). CGE uses laser-induced fluorescence (CGE-LIF) for detection and can provide data...Capillary gel electrophoresis with laser-induced fluorescence of plasmid DNA in untreated capillary. Electrophoresis...

...which tissues and cells an AAV can infect) can influence an AAV’s value greatly. Applying this mantra to...and the effects of metabotropic and ionotropic influences on overall cell function. AAVs are the most appropriate...

...delivering shRNA, CRISPR/Cas9 components, and fluorescent sensors. Retroviral vector background Until ...interest is expressed together with a marker (Fluorescent protein or selection marker) allowing for selection...

...ligation product in a gel, but we can now use fluorophore- quencher pairs or qPCR-based methods to greatly..."Optimization of a ligase detection reaction-fluorescent microsphere assay for characterization of resistance-mediating...

...colorimetric ladder, visible to the naked eye; a fluorescent ladder compatible with your imaging system; or...100–1:1,000. If your antibodies come from ascites fluid, try a dilution range from 1:1,000–1:100,000. Secondary...

...Resources You May Also Like: Viral Service CRISPR Fluorescent Proteins Cancer Research Plasmids Gene Categories...homolog B I-REL, IREL RFX5 regulatory factor X, 5 (influences HLA class II expression) - RFXANK regulatory ...and standardized building blocks: 14 different fluorescent proteins, 10 mammalian promoters and enhancers...Plasmids 101: Monitoring Cell Mobility Using Fluorescent Proteins The Challenges of Cell Culture External...

...may also like... Human Kinase ORF collection Fluorescent Protein Tools CRISPR Plasmids This resource is...

... genomic variation affects genome function to influence phenotypes....

...Plasmid Collection Visualization Michael Davidson Fluorescent Protein Collection - Davidson Allen Institute...