We narrowed to 475 results for: FLU
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TypeCollection... COVID-19 SARS-COV-2, ACE2, and CRISPR tools Fluorescent Proteins from the Michael Davidson collection... multigene constructs. ONE-GO Biosensors Kit Fluorescent Proteins Mikel Garcia-Marcos 10 plasmids for ...
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CRISPR 101: Ribonucleoprotein (RNP) Delivery
TypeBlog Post...Cas9, such as visualizing genomic loci using fluorophore tagged dCas9 may require the use of plasmid or...is required, such as when a Cas9 fusion to a fluorescent protein is used to label nucleic acid targets... -
Negative Can Be Positive: Open AAV Data with Addgene
TypeBlog Post...types used for packaging viral particles (6), can influence vector performance. Open science initiatives, ...how small changes in vector administration can influence AAV expression properties. By providing many ... -
Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More
TypeBlog Post...tissues such as blood cells or neurons can be fluorescently labeled, allowing researchers to observe changes...about zebrafish behavioral studies Download the fluorescent proteins 101 eBook Read our plasmids 101 blog... -
Save Time with Transient Plant Leaf Transformations
TypeBlog Post... pL2_pSB90_2x35S::fLUC-I::tNOS & pSB166 - pL2_pSB90_tMAS::rLUC-I::pMAS. Fluorescent proteins (FP, e.g.... -
What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program
TypeBlog Post... manipulated by injected RNA and can express fluorescent proteins. This lab has created an automated tank... process is expensive. They are using a cool fluorescent indicator system to rapidly identify recombinants... -
Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus
TypeBlog Post...overlapping AAV since both of these variables influence whether cells will be co-transduced with multiple...Drawbacks: The choice of homology sequence can influence the level of transgene expression, and may need... -
Plasmids 101: Dimers and Multimers
TypeBlog Post...; Cook et al., 2020). CGE uses laser-induced fluorescence (CGE-LIF) for detection and can provide data...Capillary gel electrophoresis with laser-induced fluorescence of plasmid DNA in untreated capillary. Electrophoresis... -
Important Considerations When Using AAVs
TypeBlog Post...which tissues and cells an AAV can infect) can influence an AAV’s value greatly. Applying this mantra to...and the effects of metabotropic and ionotropic influences on overall cell function. AAVs are the most appropriate... -
Quick Guide to All Things Lentivirus
TypeBlog Post...delivering shRNA, CRISPR/Cas9 components, and fluorescent sensors. Retroviral vector background Until ...interest is expressed together with a marker (Fluorescent protein or selection marker) allowing for selection... -
When Fidelity Matters: A frank discussion about ligase fidelity
TypeBlog Post...ligation product in a gel, but we can now use fluorophore- quencher pairs or qPCR-based methods to greatly..."Optimization of a ligase detection reaction-fluorescent microsphere assay for characterization of resistance-mediating... -
Cre-Lox and Other Site-Specific Recombinases
TypeCollection...Kits You may also like… AAV Enhancer Collection Fluorescent Biosensors Chemogenetics Plasmid Collection Optogenetics...-Dependent Reporter Plasmids Incorporating a fluorescent protein into your expression vector allows you... -
COVID-19 Resources
TypeCollection... virus. It also lists several luciferase and fluorescent reporter plasmids that have been used for measuring...an Antiviral Strategy to Combat SARS-CoV-2 and Influenza. Abbott, et al. Cell. 2020 May 14;181(4):865-876... -
Deisseroth INTRSECT Collection
TypeCollection...configuration have been developed, including additional fluorophores, excitatory and inhibitory opsins , genetically-encoded...H134R)-eYFP Vcre No Dual recombinase-dependent: Fluorophores Addgene ID Plasmid Logic Sites and Mutations... -
Genomic Deletions in Mammalian Cell Lines
TypeCollection...editing efficiency, but 37 °C is acceptable. Fluorescence Activated Cell Sorting (FACS) of Transfected...for adherent cells, the cells have reached ~80% confluence. The clones have sufficiently proliferated once... -
Zhang Lab CRISPR Page
TypeCollection...This plasmid enables Cre/loxP recombination and fluorescence assisted sorting of cells and nuclei in addition...plasmid facilitates Cre/loxP recombination and fluorescence assisted sorting of cells and nuclei in addition... -
Allen Institute for Brain Science AAV Enhancer Collection
TypeCollection...Find AAV enhancer plasmids for fluorescent labeling and recombination across brain regions and populations... enhancers for specificity and brightness of fluorescent labeling across brain regions and populations... -
Plasmids for Stem Cell Research
TypeCollection...83. Capecchi Replicating EBNA1 episome Human Fluorescent-tagged EBNA1-mediated expression of human Oct3...and dosage tracking of reprogramming factors Fluorescent tagged episomals for stoichiometric induced pluripotent... -
Troubleshooting and Optimizing a Western Blot
TypeBlog Post...colorimetric ladder, visible to the naked eye; a fluorescent ladder compatible with your imaging system; or...100–1:1,000. If your antibodies come from ascites fluid, try a dilution range from 1:1,000–1:100,000. Secondary... -
Immunology Research Plasmids and Resources
TypeCollection...Resources You May Also Like: Viral Service CRISPR Fluorescent Proteins Cancer Research Plasmids Gene Categories...homolog B I-REL, IREL RFX5 regulatory factor X, 5 (influences HLA class II expression) - RFXANK regulatory ...and standardized building blocks: 14 different fluorescent proteins, 10 mammalian promoters and enhancers...Plasmids 101: Monitoring Cell Mobility Using Fluorescent Proteins The Challenges of Cell Culture External...