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We narrowed to 856 results for: HA

Showing: 281 - 300 of 856 results

  1. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    ...the growing spectrum of fluorescent proteins (FPs) has been an invaluable resource for studying the organization...sequence (like GFP) can be inserted at the DSB. CRISPR has allowed scientists to tag and light up endogenous..., and T2A-TurboGFP-PEST), and small epitope tags (HA, Myc, Strep tag II, AviTag, HaloTag, SpyTag). The...characteristics. For example, Masato Kanemaki's lab has developed a CRISPR/Cas-based system for tagging endogenous...

  2. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ... ddPCR.) qPCR Quantitative/real-time PCR (qPCR) has been used for copy number assays for years. qPCR ...and quencher to quantify specific amplicons.  Sybr has high background noise, due to the large amount of...AT-rich DNA and the length of the amplicon and it has a higher rate of false positives (Colborn, 2008; ... used to accurately call copy numbers, though it has been shown to identify trends correctly (Muhamad,...

  3. Hot Plasmids February 2024

    Type
    Blog Post
    ...FIQ or mStayGold (Ando et al., 2023). This variant has similar photostability and brightness compared to... of AAV9-X1.1 to that of AAV1. This viral vector has brain endothelial cell-specific tropism, with slightly...purify or image a recombinant protein, the FLAG tag has proven itself a good option for essentially all applications...western blots, though the original parent clone (M2) has been widely used in a variety of applications. Find...

  4. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ... disrupting normal brain function. Chemogenetics has become a popular tool in the field as it provides...chemogenetics are instead that it is noninvasive and has longer-lasting effects. Building your chemogenetic... agonist for muscarinic receptor derived DREADDs has been clozapine-N-oxide (CNO). However, concerns have...are already approved for clinical use. Varenicline has been further modified to create ultrapotent PSEMS...

  5. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...In the last decade, the use of degron tags has become increasingly popular for the modulation of endogenously...protein levels can return to normal once the drug has been removed. The degradation and reversal are rapid... its first use as a protein regulation tool, AID has gone through an additional round of evolution (AID...the system, but the general concept of the system has remained the same. An obvious drawback of the system...

  6. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    ...community has access to all of the latest resources? In response to this challenge, Addgene has launched... why recent work in the field of cancer research has focused on picking apart the complex cellular pathways...at Vanderbilt University in Nashville, Tennessee has already demonstrated that a number of novel small...

  7. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    ...host cells immediately prior to their transduction has been shown to increase AAV transduction efficiency... such cationic reagent that is commonly used and has been shown to increase transduction efficiency by...single-use aliquots. Never use a polybrene solution that has been thawed more than once. Fibronectin is another...polybrene (e.g., hematopoietic or primary cells), and has been shown to increase transduction efficiency by...

  8. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    ...regulatory elements. To solve this problem, the Zhang lab has previously used SaCas9 (1053 aa/3.2 kb) with AAV ... these options (2.7 kb with 24 nt guides), so it has the potential to eclipse any of the CRISPR nucleases...THE ORIGINAL NgAgo ARTICLE DISCUSSED IN THIS POST HAS BEEN RETRACTED AND FOLLOW UP STUDIES HAVE FAILED ...be the best option for HDR, but this possibility has not been experimentally confirmed. Researchers have...

  9. Plasmids 101: Colony PCR

    Type
    Blog Post
    ...for the presence of an insert. Traditionally this has been done with restriction enzyme digest; however... Find Empty Backbones at Addgene Each approach has advantages and drawbacks, which are summarized in...insert-specific primers but also tell you whether the insert has the correct directionality. Verifying the insert ...and are more likely to work in a PCR reaction that has bacterial debris. Use a positive control. A good...

  10. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...Fluorescence is the emission of light by a substance that has absorbed light. The emitted light is at a longer ...protein Excitation & Emission (ex/em): Each FP has its unique ex/em peak. Therefore, choose FPs that...detect the emission. For example, if your microscope has only two lasers, at 488nm and 561nm, you will not...Some proteins are very dim (e.g. TagRFP657, which has a brightness of 0.1) and this should be taken into...

  11. General Transfection

    Type
    Protocol
    ...10 min and then recheck the pH to ensure that it has not drifted. Filter the solution through a 0.22 µm...

  12. What Good Citizenship Can Do for Reproducibility in Science

    Type
    Blog Post
    ... to the original paper that everyone in your lab has been referencing for the last 10 years, and everything...communication further up the collective priority list. There has been progress—for example, a number of funders (e.g...joined Elsevier after her postdoctoral studies, and has held various editorial roles on review and primary...

  13. New and Upcoming Viral Vectors - June 2019

    Type
    Blog Post
    ...AAV2 pAAV-CaMKIIa-HA-hM4D(Gi)-IRES-mCitrine 121538  AAV5 pOTTC1484 - pAAV SYN1 HA-hM4D(Gi) 121539 ...121539  AAV5 pOTTC1596 - pAAV SYN1 HA-hM3D(Gq) New chemogenetics AAV Plasmid Serotype Name 119741  ...

  14. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    ...birth several weeks previously. While this claim has yet to be independently verified, the watching world...Kingdom’s Nuffield Council. While the Nuffield Council has recently published a report on the social and ethical...considerations, in making sure that the research has been adequately proven to be reasonably safe and ...

  15. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    ...attributes (which turned into www.fpvis.org).  That page has been very useful over the years, and when researchers...system that allows users to see when a given page has unmoderated changes and to view previous versions... online collections of FP spectra. Every protein has a page with all the information in the database relating...

  16. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

    Type
    Blog Post
    ... dronpa fusions. With these various designs, Xin has been able to develop photoswitchable Intersectin,...Intersectin, Cdc42, Raf1, Mek1, Mek2, Cdk5, and Cas9. Xin has demonstrated the utility of these FLiPs in a variety... of cells that actively produce the HMOs. Fatima has demonstrated the successful production of both types...

  18. The MTA at Addgene

    Type
    Blog Post
    ...process, though the process has changed a lot over the last twenty years! This has helped us develop a fast... request going forward.  After another scientist has placed a request for your plasmid, they must complete...

  19. Cultivating Community Science at BosLab

    Type
    Blog Post
    ...easy. Most of our equipment has been donated by local companies and BosLab has grown into an organization... biologist with a Ph.D. from UNC-Chapel Hill and has extensive experience targeting RNA with small molecules...

  20. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    ...amplified and none are lost. Each Addgene library has its own depositor transformation and amplification...product for next-generation sequencing Once the DNA has been extracted, the representation of the library... success of your amplification. I hope this post has helped clarify some of the important considerations...
Showing: 281 - 300 of 856 results