...attributes (which turned into www.fpvis.org).  That page has been very useful over the years, and when researchers...system that allows users to see when a given page has unmoderated changes and to view previous versions... online collections of FP spectra. Every protein has a page with all the information in the database relating...

...easy. Most of our equipment has been donated by local companies and BosLab has grown into an organization... biologist with a Ph.D. from UNC-Chapel Hill and has extensive experience targeting RNA with small molecules...

...amplified and none are lost. Each Addgene library has its own depositor transformation and amplification...product for next-generation sequencing Once the DNA has been extracted, the representation of the library... success of your amplification. I hope this post has helped clarify some of the important considerations...

... dronpa fusions. With these various designs, Xin has been able to develop photoswitchable Intersectin,...Intersectin, Cdc42, Raf1, Mek1, Mek2, Cdk5, and Cas9. Xin has demonstrated the utility of these FLiPs in a variety... of cells that actively produce the HMOs. Fatima has demonstrated the successful production of both types...

...AAV2 pAAV-CaMKIIa-HA-hM4D(Gi)-IRES-mCitrine 121538  AAV5 pOTTC1484 - pAAV SYN1 HA-hM4D(Gi) 121539 ...121539  AAV5 pOTTC1596 - pAAV SYN1 HA-hM3D(Gq) New chemogenetics AAV Plasmid Serotype Name 119741  ...

...birth several weeks previously. While this claim has yet to be independently verified, the watching world...Kingdom’s Nuffield Council. While the Nuffield Council has recently published a report on the social and ethical...considerations, in making sure that the research has been adequately proven to be reasonably safe and ...

...Davis School of Medicine on this grant. Dr. Trimmer has created thousands of high-quality antibodies/nanobodies...mission This is the first NIH grant that Addgene has received. Addgene prides itself on being financially...The timing is ideal as the NIH’s BRAIN Initiative has been funding laboratories, such as Dr. Trimmer’s ...

...process, though the process has changed a lot over the last twenty years! This has helped us develop a fast... request going forward.  After another scientist has placed a request for your plasmid, they must complete...

... to the original paper that everyone in your lab has been referencing for the last 10 years, and everything...communication further up the collective priority list. There has been progress—for example, a number of funders (e.g...joined Elsevier after her postdoctoral studies, and has held various editorial roles on review and primary...

...derived from the same cell type; however, their usage has been limited by technical problems. For instance,...binary systems in which transient protein expression has a long-lasting effect, such as Cre- or Cas9-mediated...effects. Third, protein purification (once a protocol has been established) may be less laborious than viral...

...sequence if necessary. The example plasmid on the right has a total size of 7.3kb, including a 1.2 kb insert....resulting gel image includes a 1kb ladder (lane 1) that has bands ranging from about 500bp to 10kb, with the ...reference point so you can easily assess how far the gel has run. Bonus: The dyes also run at predicted sizes ...

...Griesbeck of the Max Planck Institute for Neurobiology has been working on genetically encoded indicators of...imaging neurons, Griesbeck adds that this color change has a particular advantage for observing events taking...Maarten Merkx of Technische Universiteit Eindhoven has similar interest in FRET-based sensor proteins, particularly...

...booklet. What has been the most surprising thing about becoming a PI? Tom Ellis has a one word answer...prepared me for what a PI without a personal assistant has to deal with. My inbox currently stands at about ...

...means the bacterium is not naturally resistant but has become resistant somehow overtime. One fantastic ...on a plate! Antibiotic resistance can arise, and has arisen, for many different reasons. Exposure to antibacterial...presence of a known concentration of antibiotic that has been impregnated into a paper disk, and observing...resistance; it’s a natural process of life and one which has allowed bacteria to evolve and survive for millennia...

...studying the genetic underpinnings of these processes has been complicated by the lack of precise genetic tools...Simplified in vivo delivery using SPH mice CRISPRa has largely been limited to in vitro settings due to ...can be delivered to the CNS by lentivirus, which has a larger packaging capacity than AAV. Such an approach... or coding regions. Typically, each gene isoform has a distinct transcriptional start site. Because CRISPRa...

... Cas11-like protein, and a guide RNA. The enzyme has dual activities: processing pre-CRISPR RNA into mature...kilobase-sized gap in the gene editing field. The AbuGoot lab has now developed PASTE, which can “drag and drop” kilobases...PASTE is more specific and efficient than HITI and has higher purity of insertion than both HITI and HDR...therapeutic applications. The potential of CRISPR has yet to fully taken advantage of, but exciting new...

...that have been in the repository for many years, has now grown into a collection of over 2,400 plasmids... based on 100 structure-guided spike designs and has improved protein yields and stability. This stabilized...virus spreads around the world, the spike protein has had many opportunities to evolve. Several variants... to accelerate research through sharing reagents has never been so important.     References and ...

...off-target effects of gene therapy, Feng Zhang’s lab has developed a system called DIRECTED (Delivery to Intended...on-target delivery with various delivery modalities and has the potential to reduce negative side effects. This... assays like IHC. In fact, Anti-PSD-95 [K28/43R] has been one of the hottest antibodies in Addgene’s collection... collection since we began distribution and has been verified to perform well in standard IHC and western...

...plasmid transformation, though, a pooled library has hundreds to millions of distinct plasmids, all of...they were prior to amplification. And as anyone who has worked with individual plasmids knows, some plasmids...size and backbone.  Verification Once your library has been amplified, you'll need to verify your library...transduction conditions, you’re ready to transduce! Addgene has a transduction protocol you can follow and a troubleshooting...

... is easy to use, functional in most species, and has many applications. That said, SpCas9 is not the only...SpCas9. Cas13a (previously referred to as C2c2), has several unique properties that further expand the...single A, U, or C base pair. In bacteria, once Cas13a has recognized and cleaved its target RNA sequence as...This system, dubbed SHERLOCK (depicted in figure 1) has been used to differentiate strains of Zika virus,...