Addgene: The First Twenty Years
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Blog Post
...1,000 ready-to-use viral vector preps. Today, that number has grown to over 114,600 viral preps. And in 2025...
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Antibodies 101: Conjugation
TypeBlog Post...WrasidloO WA, Reisfeld RA (1988) Determination of the Number of e-Amino Groups Available for Conjugation of ... -
Viral Vectors 101: An Introduction to AAV
TypeBlog Post...host genome, and can be specifically targeted to a number of different cell types even when systemically ... -
To Each HIS Own
TypeBlog Post...histidine strings can increase that risk. Number of residues Deciding how many histidine residues... -
Plasmids 101: CcdB - The Toxic Key to Efficient Cloning
TypeBlog Post...would die. This procedure dramatically reduces the number of clones that do not contain the recombinant plasmid... -
Antibodies 101: Selecting the Right Antibody
TypeBlog Post...above steps, you may find that you still have a number of options. If this is the case, you can narrow... -
Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools
TypeBlog Post...neutral vesicles to more acidic lysosomes. Though a number of biosensors existed that met some of the criteria... -
Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute
TypeBlog Post... good storytellers in science can do.” Susan’s number one piece of advice for getting started in science... -
dTAG - You're it!
TypeBlog Post...will provide an update with vendor and catalog numbers. As we are happy to share aliquots of dTAG-13 in... -
Transferable Skills Guide: Cross-team Communication
TypeBlog Post...scientists within and outside your field, and any number of interactions with professionals from other backgrounds... -
CRISPR 101: Cas9 vs. The Other Cas(s)
TypeBlog Post...Fast facts – PAM requirement: NGG and an expanding number of others. Best for: a wide range of DNA edits... -
Lighting Up Cell Signaling with Photoswitchable Kinases
TypeBlog Post...are photoswitchable kinases good for? There are a number of potential applications for photoswitchable kinases... -
Developing Lab Management Software for Biology
TypeBlog Post... you want your software to treat those encoded numbers. Loosely couple containers and contents Right from... -
Career Coaching for Scientists: Why and Where Do I Find One?
TypeBlog Post... will be a match for your needs, but here are a number I have come across for you to consider. Feel free... -
Plasmids 101: Cre-lox
TypeBlog Post...posts for our Plasmids 101 series, we examined a number of important plasmid elements – promoters, origins... -
CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9
TypeBlog Post...difficult to study ncRNA, CRISPR systems offer a number of different options. By carefully considering ... -
Viral Vectors 101: Calcium Sensors
TypeBlog Post...from troponin C. It is engineered with a reduced number of calcium binding sites per sensor (to decrease... -
Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation
TypeBlog Post...Morita et al. 2016, Xu et al. 2016). But there are a number of drawbacks to this approach: TET proteins are... -
Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene
TypeBlog Post... entirely remains a large undertaking. Priority number one was to ensure that this changeover had no impact... -
CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems
TypeBlog Post...the type of CRISPR-Cas system they block and are numbered in order of discovery (Bondy-Denomy et al., 2018...