We narrowed to 651 results for: PPL
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TypeBlog Post...an organism’s genome usually for a research or applied goal. Components of CRISPR: gRNAs: Sequences ...produce either a gRNA, a nuclease, or both. Applications of CRISPR: Cut: CRISPR can be used to cut the...
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Kwabena Duedu on Public Health, Biofuels, and Doing Science in Ghana
TypeBlog Post...awarded plasmids from Addgene as a successful applicant to the Addgene, Seeding Labs Plasmid Grant. For...scientist's molecular biology needs and why Kwabena applied for the Addgene, Seeding Labs plasmid grant. 7:... -
Editor's Choice, July 2016
TypeBlog Post...target and use FRET flurophores for a variety of applications. While editing this post, I learned a lot about...this post to anyone wondering if and how they can apply FRET in their own research. All posts from July ... -
Introducing an All-in-One CRISPR/Cas9 Vector System for Multiplex Genome Engineering
TypeBlog Post...that it is also possible that their system may be applied to efficient viral vector-mediated multiplex genome...genes at the same time.” For more details on how to apply the new system, see Yamamoto and Sakuma’s protocol... -
Keeping Up With CRISPR/Cas9
TypeBlog Post...significant advances in the understanding and application of CRISPR/Cas9 technology are coming along at...of Chase L. Beisel and Rodolphe Barrangou, had applied the CRISPR/Cas9 system to developing an antibiotic... -
Microbes: Look, Listen, and Tell
TypeBlog Post...cellular imaging with ultrasound. In his research he applies naturally occurring bacterial “molecular buoyancy...biomolecule-based dyes. This technology is widely applicable as bacterial expression of dyes allows dye visualization... -
Designer PUF Proteins for Any RNA Target
TypeBlog Post... The new tool was developed and implemented by applying the Golden Gate cloning method to human proteins...says the new tool might even find therapeutic application in addition to their use in basic biology, for... -
Searchable and Sortable gRNAs for Your Next CRISPR Experiment
TypeBlog Post...with the gRNA), application, cas9 species, PubMed ID, and depositor. The CRISPR applications we currently... -
Seeing Red: Simple GFP Photoconversion
TypeBlog Post...Since the first research applications of GFP were published in the 1990s, biologists have spent a lot... Sattarzadeh et al., Sci Rep. 2015 CC BY 4.0 Applications and limitations of GFP photoconversion One concern... -
New Norepinephrine Indicators: nLightG and nLightR
TypeBlog Post...neurons expressing nLightG or nLightR before/after application of NE (10 μM) and corresponding pixel-wise ΔF...GFP), and nLightR uses a red fluorescent protein, mApple. Kagiampaki et al. found nLight is suitable for... -
Future of Research Conference - Remarkable Opening Session
TypeBlog Post... value and priority put on good mentoring and applauded the new requirement for PIs to include in their... of funding basic research at NEB and got some applause when he talked about how he boycotts publishing... -
Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner
TypeBlog Post...adaptive bacterial immunity and its potential applications in rewriting the genome. Their award acceptance... area will accelerate the launch of clinical applications. During the panel discussion, Dr. Mello took... -
SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture
TypeBlog Post... if there are forces acting on them. For the application we were looking at, magnetic isolation of circulating...out the cell capture system.) Cancer and other applications Addgene: What does this technology mean in the... -
The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins
TypeBlog Post...YFP) to the new and improved fruit colors (e.g., apple, papaya, and tomato) and the photoactivatable fluors...on the corresponding plasmid pages (e.g. see Supplemental Documents here). If you’re not sure where your... -
In Living Color: The Skinny on In Vivo Imaging Tools
TypeBlog Post...of hues. While those tools are great for many applications, Verkhusha and his lab at Albert Einstein College...Verkhusha’s team reported another advance in the application of near-infrared fluorescent proteins, iRFP670... -
Oh, The Places You Can Go: Careers in Science Communication - Science Writing
TypeBlog Post...should be. He debated taking a position as an application support/sales representative in industry or a...illustrations for summarizing mechanisms and applications that are relevant to things IDT does. I also... -
Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation
TypeBlog Post... al., 2019. Applications for SPARC SPARC technology is versatile and can be applied across cell types... -
Early Career Researcher Toolbox: Free Online Molecular Biology Tools
TypeBlog Post...making it ready to copy and paste into other applications Primer design tools Many of the above-listed... well as primer design for different cloning applications. Primer3 and Primer3Plus: For general primer... -
An Addgene Summer Internship
TypeBlog Post... had spent most of my junior year stressed out applying for summer programs. I spent one day at Addgene...internship opportunity taking place next summer. I applied to Addgene and began the interview process with... -
Choosing Your Perfect Empty Backbone
TypeBlog Post...types of yeast expression vectors. Consider your applications and experimental design. You can create a new...a variety of other types of vectors for most applications used to study gene function. From making transgenic...