We narrowed to 365 results for: Pol

1. Magnetic Control of Proteins: More than a Dream

   Type

   Blog Post

   ...References and resources References Duret, G., Polali, S., Anderson, E. D., Bell, A. M., Tzouanas, C....

2. Viral Vectors 101: Producing Your rAAV

   Type

   Blog Post

   ...rAAV from your supernatant, you can simply use polyethylene glycol (PEG). We add 25 mL of 40% PEG solution...

3. Truncated gRNAs for Regulating Gene Expression

   Type

   Blog Post

   ..., Cas9 forms a steric block that prevents RNA polymerase from efficiently transcribing the gene. This ...

4. Hot Plasmids and Viral Preps - May 2021

   Type

   Blog Post

   ...transporting proteins in yeast by using murine polyomavirus virus-like particles (MPyV VLPs ). This artificial...

5. CRISPR 101: Mammalian Expression Systems and Delivery Methods

   Type

   Blog Post

   ...Chemical transfection   Lipid mediated Cationic polymers Calcium phosphate Cells Transformed cell lines...

6. Light Sheet Fluorescence Microscopy

   Type

   Blog Post

   ...Praparaten. Leipzig: S. Hierzel.
 J.A Dent, A.G. Polson, M.W. Klymkowsky. (1989) Development, 105.61-74...

7. CRISPR 101: Ribonucleoprotein (RNP) Delivery

   Type

   Blog Post

   ...intense study. Two methods to accomplish this are Polyethylene Glycol (PEG) mediated transfection of protoplasts...

8. Uncovering Ancestral Bias in CRISPR Research

   Type

   Blog Post

   ...have approximately 20% more single nucleotide polymorphisms (SNPs) that map to guides in the Broad’s Genomic...

9. An Introduction to Adenovirus

   Type

   Blog Post

   ...initiating the whole process – such as the viral DNA polymerase – it makes sense that vectorization strategies...

10. Droplet Digital PCR for AAV Quantitation

    Type

    Blog Post

    ...resources on the Addgene blog Read more about polymerase chain reaction Browse our blog posts related ...

11. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type

    Blog Post

    ... these fluorophores are often bulky and contain polar groups (Figure 2b), which can preclude their use...

12. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type

    Blog Post

    ...has four layers of location which can all be extrapolated from the 2D barcode and LIMS: Barcoded Tube...

13. Deciphering the Mysteries of Behavior with Viral Vectors

    Type

    Blog Post

    ... psychiatric diseases such as schizophrenia or bipolar disorder. In both diseases, animals have reduced...

14. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

    Type

    Blog Post

    ..., including investigations into environmental pollutants and health, embryo growth, brain function, and...

15. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

    Type

    Blog Post

    ...Wieschaus. "Mutations affecting segment number and polarity in Drosophila." Nature287.5785 (1980): 795-801...

16. Lab to Office Culture Shock

    Type

    Blog Post

    ...of the causes of culture shock above, but after polling some Addgene colleagues, we’ve come up with a list...

17. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type

    Blog Post

    ...Split inteins are a pair of naturally occuring polypeptides that, when at the ends of two proteins, mediates...

18. Transferable Skills Guide: External Collaborations

    Type

    Blog Post

    ...skill is a hallmark for jobs focusing on: Science Policy, Business Development, Administration and Intellectual...

19. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type

    Blog Post

    ...engaging target DNA. The reverse transcriptase polymerizes DNA onto the target strand based on the sequence...

20. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type

    Blog Post

    ...interest (red) is amplified using Recombinase Polymerase Amplification (RPA) or Reverse-transcription ...