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We narrowed to 582 results for: RET

Showing: 281 - 300 of 582 results

  1. Of Myc and Men

    Type
    Blog Post
    ...studies investigating mechanisms of endoplasmic reticulum localization, Munro and Pelham casually introduced...Pelham HRB (1987) A C-terminal signal prevents secretion of luminal ER proteins. Cell 48:899–907. https...

  3. Tips for arabidopsis transformation

    Type
    Blog Post
    ... (Kyndt et al., 2015)! It sounds simple, and it pretty much is, but there are some tips and tricks you...having an analyzable transgenic in hand, so it is pretty important to get this process right the first time...

  5. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    ...within the target window, possibly due to longer stretches of duplexed RNA. Using an ADAR2DD catalytic mutant...perform C-to-U edits, the authors note that it retains its adenine deaminase ability. The authors then...

  7. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    ... great idea to most people in 2005, but XKCD is pretty successful!  What resources do you have?  No matter...for children” or may need text for audience interpretation. Infographics Low Combines both words and...

  8. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...Komagataella phaffii (a.k.a  Pichia pastoris) because it secretes fewer of its own proteins, making the purification...production of Fab fragments where variable chains are secreted into the periplasmic space to be oxidized and ...

  9. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ... mechanism to culture only bacteria which have retained a functional copy of your plasmid of interest,... plates due to the fact that beta-lactamase is excreted. Satellite colonies are small colonies of bacteria...

  10. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...appear reduced in ExM, which could impact how you interpret a target’s structure. For example, cytoskeletal..., Chen F, Piatkevich KD, et al (2016) Protein-retention expansion microscopy of cells and tissues labeled...

  11. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    ...proteins were successfully expressed as fusions that retain their individual activities (1). Researchers also...DBD) and transcriptional activation (TA) domains retain their individual activities when separated (2),...

  13. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ...known 'DNA adenosine deaminases' which would theoretically give rise to an adenine base editor. A massive...developed using the hAPOBEC3 deaminase can edit in a stretched window ranging from 1-17 bp relative to the PAM...

  14. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...antibodies. In contrast, with their many epitopes, smFPs retain their ability to be detected after sample preparation...the Addgene Blog Rabies and neuronal tracing Retrograde AAV: Making the journey from axon to nucleus ...

  15. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    ...typically contains one viral genome. Other than a theoretical maximum value, physical titers don’t necessarily...different sources in the same experiment, consider retitering both AAV in your lab. The absolute titer values...

  16. Hot Plasmids - August 2020

    Type
    Blog Post
    ...exported to the periplasm of bacteria where it can be secreted into the supernatant. Four plasmids containing...in human pluripotent stem cells(hPSCs), Lindy Barrett’s lab at the Broad Institute developed fluorescently...

  17. Transferable Skills Guide: Creativity

    Type
    Blog Post
    ...kill your productivity. Harvard Business Review. Retrieved January 14, 2022, from https://hbr.org/2019/11...innovation on your team. Harvard Business Review. Retrieved January 14, 2022, from https://hbr.org/2019/07...

  18. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    ...cell’s genome, so no special sequence is needed to retain the vector as cells divide. In transient transfection... and eukaryotes. Conclusion I still think it’s pretty incredible that my cells “speak” the same molecular...

  19. How to Be an Excellent Trainee

    Type
    Blog Post
    ...trainee (even if you’re an oldbe trainee!) can be pretty nerve-wracking. So how can you be the best trainee...gives twofold benefits: you may learn faster and retain more information plus your mentor will get you ...

  20. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...faculty, students, private sector researchers, or pretty much anyone applying the scientific method to a..., also known as the science videographer, is a retired botanist and oceanographer who has a blog and several...
Showing: 281 - 300 of 582 results