We narrowed to 601 results for: RET
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TypeBlog Post...pGP-AAV-syn-jGCaMP8f-WPRE (AAV Retrograde) pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE (AAV Retrograde) pGP-AAV-syn-FLEX-jGCaMP8m-WPRE...
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Filming Science Videos in the Age of Social Distancing
TypeBlog Post...composition. So back to the drawing board. Cycle 3: A Return to HQ Figure 3: Even Blugene is practicing...acquire gear that’s only good once. Even when things return to relative normalcy, I’ll be incorporating that... -
Five Popular Model Organisms
TypeBlog Post... (Zuber et al., 2009). By injecting mice with retroviral vectors that harbored different mutations found...21415126. PubMed Central PMCID: PMC3082451. Pfeiffer, Barret D., et al. "Refinement of tools for targeted gene... -
Plasmids 101: Biotinylation
TypeBlog Post...papers and webpages are listed below: EMSA (Electrophoretic Mobility Shift Assays) ELISA (Enzyme-Linked...Schwartz, S. A. “Biotinylated probes in the electrophoretic mobility shift assay to examine specific dsDNA... -
Transferable Skills Guide: Time Management
TypeBlog Post...entire experiment, but you’ll have to spend the entirety of each day doing the experiment. The second way...during my day according to my priorities, I have a pretty good idea about the specific things I’ll be doing... -
Plasmids 101: Dimers and Multimers
TypeBlog Post...that affect the frequency of multimerization. Theoretically, as long as all the plasmid features remain ... self-control and a sense of direction are the secrets of multicopy plasmid stability. Mol Microbiol. ... -
CRISPR Plasmids - RNA Targeting
TypeCollection...Marker PI Publication Return to top Bacteria ID Plasmid Gene/Insert PI Publication Return to top Plant ID Plasmid... Plasmid Gene/Insert PI Publication Return to top CRISPR Resources Addgene has a large selection of CRISPR... -
CRISPR Plasmids - Purify Genomic Loci
TypeCollection...Publication Return to top Bacteria ID Plasmid Gene/Insert Promoter Selectable Marker PI Publication Return to ...Insert Promoter Selectable Marker PI Publication Return to top CRISPR Resources Addgene has a large selection... -
CRISPR Plasmids - Cascade-Cas3
TypeCollection... Publication Return to top Bacteria ID Plasmid Gene/Insert Promoter PI Publication Return to top Plant... ID Plasmid Gene/Insert Promoter PI Publication Return to top CRISPR Resources Addgene has a large selection... -
Antibodies 101: Antibody Engineering and Directed Evolution
TypeBlog Post...good solution. The process of error-prone PCR is pretty much exactly what it sounds like — a PCR, but with...antibodies that target families of cell surface and secreted proteins (glypicans, integrins, and more coming... -
Antibodies 101: Validation
TypeBlog Post...you see that there is high RNA expression in the retina and in parts of the brain, but almost none in the..., you will see a strong antibody signal in the retinal sample, but little to no signal in the liver sample... -
A Guide to Getting Started in Undergrad Research
TypeBlog Post... try research? Personally, I have always been a pretty curious person. I like to ask questions and think...that you don’t think you’re qualified for. Wayne Gretzky’s “You miss 100% of the shots you don't take” quote... -
Mycoplasma Contamination: Where Does It Come From and How to Prevent It
TypeBlog Post... is required in this process because result interpretation might be difficult. The low density of mycoplasmas...by these bacteria can hinder correct result interpretation. Also, the signals from the nuclear area make... -
Serotype Testing AAV
TypeCollection... AAV2, AAV5, AAV6, AAV8, AAV9, AAV11, and AAV retrograde....AAV11 37825-AAV11.T 20 µL $ 150 Add to Cart AAV Retrograde 37825-AAVrg.T 20 µL $ 150 Add to Cart pAAV-hSyn-EGFP...AAV11 50465-AAV11.T 20 µL $ 150 Add to Cart AAV Retrograde 50465-AAVrg.T 20 µL $ 150 Add to Cart *Each viral... -
SARS-CoV-2 Pseudotyped Virus
TypeCollection...HIV-based lentiviral particles Murine leukemia-based retroviral particles Vesicular stomatitis virus particles...Lentiviral Envelope and Packaging Plasmids Popular γ-Retrovirus Envelope and Packaging Plasmids Reporter Plasmids...Resources Ginkgo Bioworks COVID-19 Plasmid Collection Return to top Content last reviewed on 01 October 2025... -
Plasmids 101: Modular Cloning Applications and Kits
TypeBlog Post... technology has been used to optimize protein secretion and expression in the biotech workhorse yeast ...Modular Toolkit for Generating Pichia Pastoris Secretion Libraries.” ACS Synthetic Biology, vol. 6, no.... -
Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein
TypeBlog Post...entire plate was scanned for V5 tagging using an Operetta imaging system. Dewari et al, eLife 2018;7:e35069...follow when planning epitope tagging experiments: Retrieve Ensembl Transcript ID(s) of your favourite gene... -
A Primer on Optogenetics: Introduction and Opsin Delivery
TypeBlog Post... proper behavioral readout that allows you to interpret the results of your experiment. Like many things...optogenetics has already been proven. Therefore, a concrete, well defined, and robust behavioral output is... -
mTOR Pathway
TypeCollection...was generated with the help of David Sabatini . Return to top mTOR Pathway - Gene List Click on a name...sclerosis VHL Von Hippel-Lindau tumor suppressor Return to top mTORC2 Complex Symbol Name AKT AKT1 AKT2...complex 2 SGK Serum/glucocorticoid regulated kinase 1 Return to top Resources Cancer Pathway ORF Kit (Sabatini... -
Worm Expression Resources
TypeCollection..., or by suggesting plasmids for the collection. Return to top Plasmid Kits and Collections Kits are sets...synthetic biology plasmids for use in C. elegans . Return to top Other Resources Addgene Blog Posts An “elegans...genome of the genetic model organism C. elegans. Return to top Do you have suggestions for other plasmids...