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We narrowed to 741 results for: REV

Showing: 281 - 300 of 741 results

  1. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    ...methyltransferases, methylate DNA at particular sequences to prevent them from being degraded by restriction endonucleases...used for long term storage. Additional Resources Review of restriction modification systems NEB's Resource...describing Dam and Dcm Methylases Discovery of DpnI Review describing dam-/dcm- E. coli Resources on the ...

  2. Choosing the B(right)est Fluorescent Protein: Photostability

    Type
    Blog Post
    ...Advanced microscopy, University of Amsterdam. The previous post in this series described a practical approach...photostable fluorescent protein. Photobleaching is the irreversible destruction of a fluorophore under the influence...photobleaching, fluorescent proteins may display reversible intensity changes (Shaner et al, 2008; Bindels...

  3. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...next-generation sequencing. Sequencing the 16S rRNA reveals the identity of the organisms present while sequencing...16S rRNA isolated from the microbial community) reveals the RBS and allows translation of the reporter ...Cameron, et al. "A distributed cell division counter reveals growth dynamics in the gut microbiota." Nature ...

  4. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    ... are two heart defects that are 50 times more prevalent in women with TS. “The goal of our study is to...chromosome gene dosage in TS cells as a first step to reverse the developmental defects that cause heart problems...monitor intracellular signaling,” says Hébert. Previous research has shown that GNB1 mutations lead to...

  5. Click Biology: How to Click and Play to Explore Biology

    Type
    Blog Post
    ...Chemistry. Chemical Reviews, 121(12), 6697–6698. https://doi.org/10.1021/acs.chemrev.1c00469  Howarth,...plasmids Exchangeable HaloTag Ligands HaloTag Reversible binding of fluorescent ligands to HaloTag PCV-Cas9...

  6. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    ...marriage of two simple platforms CRISPR has revolutionized how we decode the genome by making it easy ...CACCGNNNNNNNNNNNNNNNNNNNGTTTGGGTCTTCGAGAAGACCTATTCXXXXXXXXC -3’; Reverse oligo: 5’- AATTGXXXXXXXXGAATAGGTCTTCTCGAAGACCCAAACNNNNNNNNNNNNNNNNNNNC... Traver, et al. "High-Resolution CRISPR Screens Reveal Fitness Genes and Genotype-Specific Cancer Liabilities...

  8. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

    Type
    Blog Post
    ...Editing Technology at Beam Therapeutics, used a previously developed bacterial selection strategy (Gaudelli...more efficient. Informed by these mutations and previously described TadA mutations (Gaudelli et al., 2017...mitigated with the expression of fetal hemoglobin. Previous studies have shown that one base change in the...

  9. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

    Type
    Blog Post
    ...RNAs. Annual Review of Biochemistry. 2012;81(Volume 81, 2012):145-166. doi:10.1146/annurev-biochem-051410...transcription start site function would also help to reveal the impacts of a lncRNA on nearby genes and its...

  10. CRISPR in the Clinic

    Type
    Blog Post
    ...field forward to where it is today. Below we will review some of the CRISPR-based editing therapies that...’s own cells that have been modified ex vivo, previously edited healthy donor cells can be delivered immediately...safety is important for allogenic therapies to prevent a graft vs. host response. Off-target effects are...

  12. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ...therefore already have ways to prevent AAV infection. For example, prevalence of neutralizing antibodies ...or potentially curing human diseases that were previously thought to be incurable. Rapid advances in next...

  13. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    ...symposia held across the USA during this time have revealed that young scientists are aware of, and are engaged...Academy of Sciences. “The Postdoctoral Experience Revisited” 2014 Appendix B p93-95). For those advocating...25653845. Pubmed Central PMCID: PMC4304227. 4. Revitalizing biomedical research: recommendations from the...

  14. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    ...a table. It adds a formality and distance that prevents interaction with each other and the audience. ...moderator takes control It is the moderator's job to prevent anyone from derailing the show. The moderator must... active at all times keep things moving and to prevent any one panelist or audience member from taking...

  15. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    ... series from Subach et al.) were derived from previously developed monomeric fluorescent proteins mKO ...dense-core vesicle exocytosis in neuroendocrine cells revealed by newly developed monomeric fluorescent timer.... German. "Chronology of islet differentiation revealed by temporal cell labeling." Diabetes 58.8 (2009...

  16. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ...Inspired Engineering. CRISPR/Cas9 technology has revolutionized the fields of molecular biology and bioengineering...which variant of Cas9. To circumvent this problem, previous studies have proposed the concurrent use of “orthogonal...gene of interest, Cas9 forms a steric block that prevents RNA polymerase from efficiently transcribing the...

  17. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...for dicots. NC: nucleocapsid RNA chaperone, RT: reverse transcriptase. Right: T1 tomato plants that inherited...genetically-encoded voltage indicator (GEVI) than previous versions. ASAP5 can be imaged using one- or two-photon...across many cell types in varying conditions, revealing context-dependent responses and molecular patterns...

  18. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    ...editor (typically a Cas9 H840A nickase fused to a reverse transcriptase) together with a prime editing guide...sequence containing a primer binding site and a reverse transcription template (RTT). The design of the...and highly versatile genome manipulation. Nature Reviews Genetics, 24(3), Article 3. https://doi.org/10.1038...

  19. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ...is suitable for your application. We have previously reviewed many common lab E. coli strains on the blog...optimal growth, as insufficient gas exchange will prevent your cultures from reaching the desired density...

  20. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ... to repair DNA. The DNA ends are chewed back to reveal homology, allowing the strands to anneal. DNA synthesis...Using MMEJ for gene knock-in Building on the lab’s previous work, Sakuma et al. describe a detailed protocol... using much shorter sequence homologies. Abbreviated protocol for PITCh Step 1: Generate microhomologies...
Showing: 281 - 300 of 741 results