We narrowed to 763 results for: SCR

...synthesize a particular protein DNA must first be transcribed into messenger RNA (mRNA). mRNA can then be translated...template of choice and allowing these cells to transcribe, translate and modify your protein of interest...assembled in vitro using purified components of the transcription and translation machinery. These include ribosomes...

...transfer plasmid at 30 °C instead of 37 °C and then screen for ITR recombination with a SmaI or XmaI restriction...duplicated regions like the ITRs. Restriction digest screening for ITR deletion is still necessary. To save time...mL of my AAV transfer plasmid cultures and then screen with SmaI digests. I freeze the bacterial pellet...

...transduced cells whenever possible. This post will describe our workflow for viral genome sequencing (VGS)...identification, we developed custom open-access Python scripts that can perform a deeper analysis of the data....Check out our Viral Vectors Featured Topic Page Subscribe to Viral Vectors Blog Posts Learn How We Use NGS...

...vivo. A common choice for conducting genome-wide screens using CRISPR. AAV transduction Only compatible...containing gRNA and Cas9 are used in in vitro transcription reactions to generate mature Cas9 mRNA and gRNA...complexes Purified Cas9 protein and in vitro transcribed gRNA are combined to form a Cas9-gRNA complex...

...video_player "embed_player" overrideable=False, type='scriptV4', hide_playlist=True, viral_sharing=False, embed_button...into two components: 𝜃x(t) and 𝜓z(t). 𝜃x(t) describes the exponential decay process we observed, while...appears to return to normal. The model fit these two discrete phases with an estimate of 62.3% for the COVID...

...looking at your resume while they wrote the job description. You check off all the boxes - you have the desired... gaps in scientists' transferrable skills. In describing the limitations of the study, the authors noted...over email. Examine your current and past job descriptions. Scan through all the things for which you are...

...The collection can be used for screening transcription factors for cellular programming or cancer drug ...simplifies identification of the transcription factor isoform in pooled screens. This collection and library...library can be used for screening transcription factors for applications such as cellular programming or cancer...for NGS QC and bulk screening (PDF, 85 KB) TF ORF barcode dial out PCR for 10X scRNA-seq (PDF, 59.2 KB)...to simplify identification during screening. Library ID PI Description MORF library 137000, 192821, 1000000218...Browse the Zhang lab’s comprehensive human transcription factor collection, available at Addgene. The...Library The MORF library is a comprehensive human transcription factor collection that contains 1,836 genes ...

... interfere with transcription elongation. CRISPR Activation : Target the transcription start site. CRISPRa...fused to transcriptional activators that need to be within a given range of the transcription start site...validate your genetic edit. Figure 1: Flow chart describing the general framework of a CRISPR experiment....Prime editor (Cas9 nickase fused to reverse transcriptase (RT)) Can edit within a wider window than most...genome sequence dCas9 or dCas9 fusion with transcriptional repressor (such as dCas9-KRAB) dCas9-KRAB can...modifying the genome sequence dCas9 fusion with transcriptional activator (such as dCas9-VP64) Many different... interest, where the Cas9 protein can block transcription from ever initiating. gRNAs targeting regions...

...amplification) or reverse transcriptase (RT)-RPA, respectively. RPA is coupled with T7 transcription to convert amplified...2018) CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity. Science 360...

...vector with two other independently regulated transcription units. Created with BioRender.com.   Planning...contain plasmids with undesired ligations, so always screen multiple colonies after each transformation. This...beginners or those performing complex assemblies should screen a minimum of 8–10 colonies. You can check colonies...

...we probably exchanged dozens of drafts of the manuscript. This is the time to transform the document into...Resources on the Addgene Blog Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing ...Organizing Tips for Improving Your Next Manuscript 6 Tips for Grant Writing Resources on Addgene.org Check out...

...used in research, and data repositories. Below I describe how each of these kinds of connectivity facilitates...deposits will be made. The Dataverse integration is described in this video and the Figshare integration in ...from RSpace documents to external data sources (described above) further enhances the value the repository...

...restriction endonucleases in plasmid addition was first described by studies of EcoRI and Bsp6I in Escherichia coli...systems. As with the other toxin-antitoxin systems described above, one part of the pair is more stable than...toxin-antitoxin system discovered. Unlike the systems just described, the hok/sok systems consist of three components...

...Cas13a as a diagnostic tool, as discussed below. Subscribe to CRISPR Blog Posts Applications of Cas13a   ...Recombinase Polymerase Amplification (RPA) or Reverse-transcription RPA (RT-RPA), respectively. Amplified nucleotides... the collateral RNA cleavage response is well described in bacterial cells, LwaCas13a does not display...

...heterologously by bacteria inside the body. So we screened gas vesicles gene clusters from a diverse set ...expression heterogeneity required single cell screening to isolate a clonal cell line capable of producing...improvement in expression. Stabilizing the gvpA transcript with WPRE reduced the number of copies of gvpA...

...expected for postdocs in many cases should be as described in the notice of the new NRSA levels and in the...very relieved not to be going through the urgent scramble of their peer institutions. The issues facing ..., and it is becoming ever clearer that outside scrutiny of science and the academic system is increasing...

...methyltransferase — that can drive lasting transcriptional silencing (Nuñez et al., 2021). Now, in a collaboration...versions using dCas9, zinc finger proteins, and transcription activator-like effectors (TALEs), with fifteen...Weissman, J. S. (2021). Genome-wide programmable transcriptional memory by CRISPR-based epigenome editing. Cell...

... The one-pot saturation mutagenesis technique described in Wrenbeck et al is a PCR-based approach for ...library that’s ready to be tested in a functional screen of your liking. This method is similar to site-directed... screen...

...strong transcriptional terminator BBa_B1002 (from IGEM) upstream of the MCS to reduce transcriptional readthrough...worked too. Rhamnose induction relies on the transcriptional activator rhaS. To allow manipulation of bacteria...

...same fluorescent markers, Brainbow 3.1 and 3.2 (described in the same publication) are unique because by...cell type. The Confetti mouse was first used to describe the self-renewal of cells in the intestinal crypt...previous Brainbow technologies were useful for discriminating neurons within only one field of view. Tetbow...