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  1. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    ...biosensor is the GFP-LC3-RFP-LC3ΔG probe. It’s similar to the mRFP-GFP-LC3 Tandem Fluorescent Protein,...fluorescent protein oligomerization Seeing red: simple GFP photoconversion Light sheet fluorescence microscopy...

  2. Antibodies 101: Epitope Availability

    Type
    Blog Post
    ...target protein? Epitope Availability The answer is simple: epitope availability. An antibody can only bind...that has been validated for an assay that uses similar sample preparation - for instance, if you cannot...

  3. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    ...problem, Gao et al. searched for Argonaute orthologs similar to TtAgo and PfAgo, another thermophilic Argonaute...variety of mammalian cell lines, with an efficiency similar to that of Cas9. Like Cas9, NgAgo is also able ...

  4. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    ... out more than necessary for my interview. The simplest place I could think of to start was “Marketing...about marketing would be to start reading a broad simple overview, learn some jargon and key phrases and...

  5. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    ...Merkx of Technische Universiteit Eindhoven has similar interest in FRET-based sensor proteins, particularly...monitoring calcium versus zinc should require the simple swapping of binding domains, in practice optimizing...

  6. The MTA at Addgene

    Type
    Blog Post
    ...agreement, read on!  What is the MTA?  An MTA is simply a contract or agreement that states the terms of...clearly communicated. And that’s where MTAs, and similar agreements, shine.  These types of agreements allow...

  8. The Power Behind NGS Plasmid Validation: seqWell

    Type
    Blog Post
    ...sequencing instruments by enhancing the efficiency and simplicity of library prep. As part of our mission, we’ve...here is that, as more samples are sequenced simultaneously, it gets harder and harder to obtain balanced...

  10. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    ...cell and any individual AAV particle is rare. But simply getting into the cell may not always be enough ... can optimize AAV production. We’ve listed some simple steps you can take below but you should read our...

  11. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    ...a white color. While CRISPRainbow allows for simultaneous visualization of sites on different chromosomes... color detection In order to label two loci simultaneously, Ma and Pederson created another sgRNA containing...

  12. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    ...produced? Purifying exo-AAVs is actually a bit simpler than the classical purification protocol involving...summary, exo-AAVs seem to target cell types with a similar specifically as conventionally produced AAV particles...

  14. Networking Follow-up: I Met a Person. Now What?

    Type
    Blog Post
    ... you have something to ask or say. It can be as simple as “it was nice meeting you.” Include follow-up...person on LinkedIn I once helped by answering one simple question so I accepted the connection invitation...

  17. DAP arrays for multiplex gene editing

    Type
    Blog Post
    ...prime editing, achieving up to 3-loci MPE with similar high efficiencies, which is scalable for a higher...adeno-associated virus (AAV) and lentivirus, demonstrating simultaneous editing of genes against multiple genetic diseases...

  18. 5 Great Apps for Lab Life

    Type
    Blog Post
    ...pocket.   5. +AMINO- by iGreenEarth AMINO is a very simple app that packs important biological information... names have a B, J, X, or Z. Some alternative, similar apps: Findings Lab Notebook Cell Culture Split...

  19. Components of CRISPR/Cas9

    Type
    Blog Post
    ...using CRISPR/Cas9 for genome editing, researchers simply need to express a gRNA designed to direct Cas9 ...these arrays, they did not know their functions and simply called the repeated elements “direct repeats” and...
Showing: 281 - 300 of 649 results