Skip to main content

We narrowed to 670 results for: SIM

Showing: 281 - 300 of 670 results
  1. Tips for Improving Your Next Manuscript

    Type
    Blog Post
    ...spent collecting it will have been worthless. But simply writing down your data doesn’t guarantee a publication...welcoming us. Then it was time to work! Set up similarly to the online class, we met as a group for sessions...
  2. Are Hybrid Guide RNAs Right for Your CRISPR Application?

    Type
    Blog Post
    ...reducing potential off-target sites in the genome. Similar on-target efficacy. For perfect sequence matches...kinds of tools. Location of DNA substitution Similar on-target efficacy Reduced off-target activity ...
  3. Year of the Camelid: Antibody Style

    Type
    Blog Post
    ...antibodies in the serum of a camel and soon after found similar antibodies in llamas and alpacas (Figure 1). At...Suitability for in vivo experiments, due to their simple structure    sdAbs in research These advantages...
  4. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    ...problem, Gao et al. searched for Argonaute orthologs similar to TtAgo and PfAgo, another thermophilic Argonaute...variety of mammalian cell lines, with an efficiency similar to that of Cas9. Like Cas9, NgAgo is also able ...
  5. Antibodies 101: Epitope Availability

    Type
    Blog Post
    ...target protein? Epitope Availability The answer is simple: epitope availability. An antibody can only bind...that has been validated for an assay that uses similar sample preparation - for instance, if you cannot...
  6. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ... useful natural materials, such as antibiotics. Simon Moore and Paul Freemont’s lab previously developed..., this cell free system allows for a relatively simple, controlled “one-pot” reaction capable of reproducing...
  7. Celebrate Open Data Day with Addgene’s AAV Data Hub!

    Type
    Blog Post
    ...e.g. titer, injection site, animal strain used). Similar to the goodwill of Addgene plasmid depositors, ...this resource for the community. There are a few simple steps you can take to input your data for sharing...
  8. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    ...biosensor is the GFP-LC3-RFP-LC3ΔG probe. It’s similar to the mRFP-GFP-LC3 Tandem Fluorescent Protein,...fluorescent protein oligomerization Seeing red: simple GFP photoconversion Light sheet fluorescence microscopy...
  9. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    ... out more than necessary for my interview. The simplest place I could think of to start was “Marketing...about marketing would be to start reading a broad simple overview, learn some jargon and key phrases and...
  10. Hot Plasmids: FRET-Based Biosensors

    Type
    Blog Post
    ...Merkx of Technische Universiteit Eindhoven has similar interest in FRET-based sensor proteins, particularly...monitoring calcium versus zinc should require the simple swapping of binding domains, in practice optimizing...
  11. 5 Great Apps for Lab Life

    Type
    Blog Post
    ...pocket.   5. +AMINO- by iGreenEarth AMINO is a very simple app that packs important biological information... names have a B, J, X, or Z. Some alternative, similar apps: Findings Lab Notebook Cell Culture Split...
  12. Components of CRISPR/Cas9

    Type
    Blog Post
    ...using CRISPR/Cas9 for genome editing, researchers simply need to express a gRNA designed to direct Cas9 ...these arrays, they did not know their functions and simply called the repeated elements “direct repeats” and...
  13. DAP arrays for multiplex gene editing

    Type
    Blog Post
    ...prime editing, achieving up to 3-loci MPE with similar high efficiencies, which is scalable for a higher...adeno-associated virus (AAV) and lentivirus, demonstrating simultaneous editing of genes against multiple genetic diseases...
Showing: 281 - 300 of 670 results