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We narrowed to 732 results for: SON

Showing: 281 - 300 of 732 results

  1. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    ... proto-oncogenes and disrupt their expression (Robinson, 1982). To add insult to injury, retroviruses ...immunogenicity low! References and Resources References Robinson HL. Retroviruses and cancer. Rev Infect Dis. 1982...Gene and Cell-Based Therapies in the New Era of Personalized Medicine. Genes Dis. 2017 Jun;4(2):43-63. DOI...

  2. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    ... (fluorescent and luminescent) worlds Forster Resonance Energy Transfer (FRET) is the transfer of energy...which then emits it as light. Bioluminescence Resonance Energy Transfer (BRET) works the same way, except...tips below! This post was originally written by Jason Niehaus in June 2014 and was updated by Emily P....

  3. Hot Plasmids - May 2022

    Type
    Blog Post
    ...error rate for long sequence knock-ins by: Lucie Wilson Le Cong’s lab has developed a method using dCas9...error rates overall ranged from 0.3% to 10%, in comparison to the 62-90% error rate seen with Cas9 editors...barcodes for live cell clonal multiplexing by: Rachel Leeson Multiplexed images in live cells remains a challenge...

  4. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ... Cpf1 for multiplex genome editing.  For a few reasons, Cpf1 is a simplified system for editing multiple...guide page for a review of Cas9.  For a brief comparison of Cpf1 vs. Cas9, see the table below. Table...expression vector.  See the graph in figure 2 for a comparison of editing frequency resulting from transfection...

  5. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

    Type
    Blog Post
    ... the genome. Mendenhall and Richard Myers of HudsonAlpha (where Mendenhall is also an adjunct faculty ...the function of the protein. Myers: We’ve done comparisons of our new system versus standard ChIP-seq for... for transcription factors, but there are many reasons you might want a system like this. It’s actually...

  6. CRISPR 101: Non-Homologous End Joining

    Type
    Blog Post
    ... Renaud J-B, Sallmyr A, Ruis B, Oh S, Tomkinson AE, Hendrickson EA, Giovannangeli C, Jasin M, Brunet E...P, Burkhalter MD, Oh S, Qaqish BF, Moore DT, Hendrickson EA, Ramsden DA (2014) The fidelity of the ligation...

  7. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type
    Blog Post
    ...want to cover during the year. Suggest that each person bring 3 topics and write them on a board to see... and meet regularly no matter what, even if one person can’t make it. One of the biggest group mentoring...meetings. Experiment with the process. Have each person write a Development Plan. Set concrete goals with...

  8. Will You Be My Mentor? Finding and Asking for Mentoring Support

    Type
    Blog Post
    ... on what makes them that way. Is there a senior person whose career path you are interested in following.... Finding potential mentors is one of the many reasons to always be growing your network. If you don’t...need for your current position Understand your interpersonal challenges if they know some of the people you...

  9. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    ...decades to generate knock-out mice, but for various reasons it is not nearly as efficient in human stem cells...genetic background and do an apples-to-apples comparison of all those mutations. If you make induced pluripotent...backgrounds. It becomes more of an apples-to-oranges comparison. Addgene: Can you compare the TALEN kit to CRISPR...

  11. To Each HIS Own

    Type
    Blog Post
    ...Abrahrnsen L, Oesterlof B, Josephson S, Oestling M, Enfors SO, Persson I, Nilsson B and Uhlen M. Large-scale...

  12. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    ...ligase-mediated method, the more recently developed Gibson Assembly Cloning and Gateway® cloning technologies... toxic protein (CcdB) that acts as a DNA gyrase poison, locking up DNA gyrase with broken double stranded...killing by the F plasmid CcdB protein involves poisoning of DNA-topoisomerase II complexes.” J Mol Biol...

  14. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    ...spike protein, called HexoPro, was developed by Jason McLellan’s lab. This version of the spike protein... Lee AG, Liu Y, Chou C-W, Byrne PO, Hjorth CK, Johnson NV, Ludes-Meyers J, Nguyen AW, Park J, Wang N, ...Seaman MS, Schmidt AG, Chen B, Neuberg D, Bajic G, Harrison SC, Wesemann DR (2021) Memory B cell repertoire...

  16. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ...at chromosome 19q13.4 qtr. (AAVS1)). For these reasons, AAV is usually classified as a BSL-1 safety level...General viral handling safety   Containment and personal protection are key to lab safety and the same ...reduce the risk of an accident (the most common reason for lab accidents is not following safety protocols...

  17. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ... both NeuroMab and the Allen Brain Atlas. The comparison with two atlases enables us to see the reproducibility...literature suggest, providing an expectation and comparison on the antibody signal’s location. If a distinct... discuss the results together, explaining our reasoning to determine if the antibody is negative (poor...

  18. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    ... of tea, and you are more of an audio or visual person. Why don’t you record a podcast or put some effort...yourself as an educator for a short, animated TED-Ed lesson. 6) Network – Mingle with pros Where do you meet...Communication. In the US, UC Santa Cruz, MIT, UW Madison, and Boston University offer science communication...

  19. 6 Tips for Grant Writing

    Type
    Blog Post
    ...to non-scientists and may make your perfectly reasonable paragraph unintelligible to a lay audience. ...HR on the judging panel. It’s important for this person to get a clear and concise explanation as to why...Writing Professional Facilitate Career Planning and Personal Development with an IDP Get Tips on Keeping Up...

  20. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ...PMID: 14723844. 3. Atasoy D, Aponte Y, Su HH, and Sternson SM. 2008. A FLEX switch targets Channelrhodopsin...PubMed Central PMCID: PMC2593125. 4. Saunders A, Johnson CA, Sabatini BL. 2012. Novel recombinant adeno-...Wenbiao Chen Liquin Luo Massimo Scanziani Scott Sternson Roger Tsien Naoshige Uchida Ian Wickersham Hongkui...
Showing: 281 - 300 of 732 results