We narrowed to 472 results for: Sele

1. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

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   Blog Post

   ...co-opted to control gene expression, he could then selectively manipulate only GFP-labeled cells. Once Tang ...

2. New Tool for Lineage Tracing: The ClonTracer Library

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   Blog Post

   ...resistant to drug treatment and that these are selected for during the treatment process. The authors ...

3. 5 Steps to Writing Grant Proposals for Grad Students

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   Blog Post

   ...eligibility information, submission information, selection criteria, and award administration information...

4. A Look at Addgene's QC Process

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   Blog Post

   ...information provided and vector type  Any tags, selectable markers, and fusion proteins listed for each ...

5. Design Tips for Prime Editing

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   Blog Post

   ... by the developer of PRIDICT, which helps users select pegRNA designs that are most likely to drive efficient...

6. Plasmids 101: The Wide World of Natural Plasmids

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   Blog Post

   ...them off. While antibiotic resistance-mediated selection is indispensable for cloning work, antibiotics...

7. Antibodies 101: Choosing the Right Isotype

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   Blog Post

   ... 2).      Table 2: Secondary antibody selections for a four-antibody panel, staining in parallel...

8. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

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   Blog Post

   ...removable dCas9 expression and pLM-CMV-R-Cre. Selection for cells stably and highly expressing these constructs...

9. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

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   Blog Post

   ...contain an IRES-Puro marker for stable cell clone selection. For more information on these plasmids and a ...

10. Fluorescent Proteins 101: Introduction to FRET

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    Blog Post

    ...even apply forces (Gayrard and Borghi 2016) to a selected protein. Overcoming current limitations to permit...

11. Overcoming the Challenges of Lentiviral Production

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    Blog Post

    ...produce virus of a usable titer, or fine-tuning selection and expression in your target cell line, researchers...

12. Popular Retroviral Vectors and Their Uses in Scientific Research

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    Blog Post

    ...Cre-dependent gene expression, gene knockout, and selection marker removal. CRISPR technology pdCas9-humanized...

13. A Guide to Designing a Scientific Poster: Content, Layout, and More

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    Blog Post

    ...poster to the bottom. Depending on the color you select for the background, you’ll want to make sure that...

14. Open Resources and Plasmid Tools For Studying C. elegans

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    Blog Post

    ...for introducing point mutations with unc- 119 + selection." MicroPublication 1 (2018): 2–3. Wang, Han, et...

15. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

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    Blog Post

    ...typically with an antibody. This can artificially select against PPIs that aren't stable outside the native...

16. Hot Plasmids - October 2020

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    Blog Post

    ..., Xue Han’s lab has developed AAV vectors to selectively visualize excitatory and inhibitory synapses ...

17. Tips for Titering Your Lentiviral Preps

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    Blog Post

    ...infection (MOI) to ensure only one copy integrates, selecting by antibiotic-resistance or single cell sorting...

18. Illuminating Epigenetics with A FRET Based Biosensor

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    Blog Post

    ...methyllysine binding domain (chromodomain), which binds selectively to lysine-methylated peptides. This construct...

19. Plasmids 101: Degron Tags

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    Blog Post

    ...protein of interest, it can be degraded by this selective degrader. The novel degrader, dTAG13, is a small...

20. Antibodies 101: Conventional vs Spectral Flow Cytometry

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    Blog Post

    ... resources on the Addgene blog Antibodies 101:Selecting the Right AntibodyAntibodies 101: Secondary AntibodiesAntibodies...