We narrowed to 466 results for: Sele

1. Fluorescent Proteins 101: Introduction to FRET

   Type

   Blog Post

   ...even apply forces (Gayrard and Borghi 2016) to a selected protein. Overcoming current limitations to permit...

2. Illuminating Epigenetics with A FRET Based Biosensor

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   Blog Post

   ...methyllysine binding domain (chromodomain), which binds selectively to lysine-methylated peptides. This construct...

3. Overcoming the Challenges of Lentiviral Production

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   Blog Post

   ...produce virus of a usable titer, or fine-tuning selection and expression in your target cell line, researchers...

4. Popular Retroviral Vectors and Their Uses in Scientific Research

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   Blog Post

   ...Cre-dependent gene expression, gene knockout, and selection marker removal. CRISPR technology pdCas9-humanized...

5. A Look at Addgene's QC Process

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   Blog Post

   ...information provided and vector type  Any tags, selectable markers, and fusion proteins listed for each ...

6. Design Tips for Prime Editing

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   Blog Post

   ... by the developer of PRIDICT, which helps users select pegRNA designs that are most likely to drive efficient...

7. Plasmids 101: The Wide World of Natural Plasmids

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   Blog Post

   ...them off. While antibiotic resistance-mediated selection is indispensable for cloning work, antibiotics...

8. Open Resources and Plasmid Tools For Studying C. elegans

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   Blog Post

   ...for introducing point mutations with unc- 119 + selection." MicroPublication 1 (2018): 2–3. Wang, Han, et...

9. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

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   Blog Post

   ...removable dCas9 expression and pLM-CMV-R-Cre. Selection for cells stably and highly expressing these constructs...

10. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

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    Blog Post

    ...contain an IRES-Puro marker for stable cell clone selection. For more information on these plasmids and a ...

11. Antibodies 101: Choosing the Right Isotype

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    Blog Post

    ... 2).      Table 2: Secondary antibody selections for a four-antibody panel, staining in parallel...

12. A Guide to Designing a Scientific Poster: Content, Layout, and More

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    Blog Post

    ...poster to the bottom. Depending on the color you select for the background, you’ll want to make sure that...

13. Hot Plasmids - October 2020

    Type

    Blog Post

    ..., Xue Han’s lab has developed AAV vectors to selectively visualize excitatory and inhibitory synapses ...

14. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

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    Blog Post

    ...typically with an antibody. This can artificially select against PPIs that aren't stable outside the native...

15. Tips for Titering Your Lentiviral Preps

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    Blog Post

    ...infection (MOI) to ensure only one copy integrates, selecting by antibiotic-resistance or single cell sorting...

16. Light Sheet Fluorescence Microscopy

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    Blog Post

    ...Stelzer’s group described the single-plane or selective-plane illumination microscope (SPIM). In order...

17. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

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    Blog Post

    ... a fitness cost and are eliminated by natural selection, gene drives distort inheritance in their favor...

18. Truncated gRNAs for Regulating Gene Expression

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    Blog Post

    ...challenge of attempting to simultaneously and selectively induce both cutting and regulation of expression...

19. Plasmids 101: Degron Tags

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    Blog Post

    ...protein of interest, it can be degraded by this selective degrader. The novel degrader, dTAG13, is a small...

20. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

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    Blog Post

    ...started, too much time for this project was spent selecting guides, designing primers, and running analyses...