We narrowed to 612 results for: Set

1. Antibody Validation for Flow Cytometry

   Type

   Blog Post

   ...that is, an antibody shown to perform well in one set of conditions may not perform as well in other conditions...cell types of interest. By correlating your data sets, you can increase confidence that the labeling observed...available. Various proteomic and transcriptomic datasets can be used to select a candidate cell line (e.g...

2. Lentivirus ddPCR Titration

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   Protocol

   ...remove virus from pipette tip. To mix the dilution, set the P200 pipette to 200 µL and pipette 10–20 times...ddPCR plate onto a chilled 96-well freezer block and set aside in the BSC to cool. Prepare the RRE/RPP30 master... Droplet Reader. Open the QuantaSoft software to set up a new plate layout. Designate the sample name,...calculate the titer. Calculations In the experimental setup above, the following virus dilutions were used to...factor v = Virus volume, mL *In the experimental setup above, the cells per well is 300,000 and the virus...

3. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

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   Blog Post

   ...). A control RFP expression plasmid.  With this set up, all successfully transfected cells will express...

4. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

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   Blog Post

   ...applications and targeting scope of ABEs David Liu’s lab set out to evolve ABEs with an increased compatibility..., can only target certain sites, or make only a subset of base substitutions.  The need for speed: base...

5. Developing a cold tolerant E. coli using specialized chaperones

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   Blog Post

   ... welcome changeover from conventional academic settings, these are an eye-opening experience for cultivating...

6. Visualizing Protein Turnover In Situ

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   Blog Post

   ... not known why or how this happens. Therefore, I set out to study how protein turnover is regulated in...and specific antibody and the proper microscope setup we can observe the whole population of one particular...

7. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

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   Blog Post

   ...mitochondria, because cPYP binds to BoPD. A similar set of newly identified light- and dark- binder plasmid...Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids...genes-of-interest (GOI) into two independent expression cassettes on a single plasmid (Grefen and Blatt, 2012). ...

8. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

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   Blog Post

   ...Despite how easy these experiments are to physically set up in the lab, RNAi is often completely ineffective... Every few months we highlight a subset of the new plasmids in the repository through our hot plasmids...sequencing, revealing that they bind to a much larger subset of protein motifs than previously thought. Sidhu...

9. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

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   Blog Post

   ...interest into the chromosome. But this has its own set of problems. Rapidly dividing cells initiate genome...

10. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

    Type

    Blog Post

    ...with MG-2I-dL5** in two different experimental settings: HEK293 cell culture and in live zebrafish. For... Have you ever wanted to selectively kill a subset of cells in your model system? Turns out that with...

11. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type

    Blog Post

    ...tools and data that indicate the performance of (a set of) fluorescent proteins under specific conditions...

12. Summer SciComm: Preprints

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    Blog Post

    ...submitting patent or grant applications; to informal settings like lab meetings, Twitter, coffee chats with ...

13. Viral Vectors 101: Biosensors

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    Blog Post

    ...the basics of biosensors and how to use viruses to set up these systems. Components of a biosensor Some...

14. Viral Vectors 101: Types of viruses

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    Blog Post

    ...therapy and is currently being used in clinical settings. AAV is also a cool tool in the laboratory! It...we would all like to steer clear of! However, a subset of viruses are commonly used in the lab as tools...

15. Viral Vectors 101: Viruses as Biological Tools

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    Blog Post

    ...stably integrate their genome into the host genome, setting up permanent residence. They primarily infect dividing...

16. Antibodies 101: Polyclonal Antibodies

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    Blog Post

    ...compare to other antibody options out there, you’re set up for a successful next experiment!     References...

17. New Tool for Lineage Tracing: The ClonTracer Library

    Type

    Blog Post

    ...population (all barcodes can be amplified using the same sets of forward and reverse primers). The abundance of...

18. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

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    Blog Post

    ...for resource allocation. Those will allow you to set the amount of transcriptional and translational activity...Christopher Voigt explains it, his lab at the Massachusetts Institute of Technology has been “working on...

19. Transfection for Recombinant Antibodies

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    Protocol

    ...counter 37 °C, 5% CO 2 incubator with shaking platform set to 120 rpm 37 °C bead bath Vortex Stir bar Magnetic...a 37 °C, 5% CO 2 incubator on a shaking platform set to 120 rpm. Pro-Tip Do not use cells that are over...into a clean microcentrifuge tube. Pro-Tip Cells settle quickly and need to be resuspended before sampling...

20. RNA Extraction Without A Kit

    Type

    Blog Post

    ...extraction. Researchers at the NIH in the mid 1980’s set out to develop a protocol that skipped the ultracentrifugation...