Hot Plasmids: Fall 2024
Type
Blog Post
...//doi.org/10.57733/addgene.l0ulu4.
Hanke, T., Szawlowski, P., & Randall, R. E. (1992). Construction of...
We narrowed to 305 results for: Ski;
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Designing Your Chalk Talk for the Academic Job Interview
TypeBlog Post...presenting a science talk Check out our transferable skills guide Read all of our career blog posts Resources... -
Antibodies 101: Introduction to Immunofluorescence
TypeBlog Post...www.ibiology.org/talks/fluorescence-microscopy/ Uhlen M, Bandrowski A, Carr S, Edwards A, Ellenberg J, Lundberg E... -
Filming Science Videos in the Age of Social Distancing
TypeBlog Post...our educational videos. In addition, it’s really asking a lot of someone to be comfortable on camera while... -
Tips for Getting a Faculty Position
TypeBlog Post...expensive habits of running shoes and single malt whiskies, but I remember the days of modest postdoc salaries... -
Plasmids 101: Expressing shRNAs for RNA Interference
TypeBlog Post...Drosha to pre-miRNA. miRNA plasmids are engineered to skip this first pri-miRNA step. These RNA duplexes bind... -
Viral Vectors 101: Integrase-Deficient Lentiviral Vectors
TypeBlog Post...Schwarzwaelder, K., Bartholomae, C., Hubank, M., Kempski, H., Brugman, M. H., Pike-Overzet, K., Chatters... -
Hot Plasmids: Summer 2024
TypeBlog Post... S., Davis, J. R., Newby, G. A., Chen, P. Z., Palczewski, K., & Liu, D. R. (2024). Engineered virus-like... -
How to Design Your gRNA for CRISPR Genome Editing
TypeBlog Post...R, Ragavendran A, Brand H, Erdin S, Cowan CA, Talkowski ME, Musunuru K (2014) Low Incidence of Off-Target... -
Exploring Applications of the Bioluminescent HiBiT Tag
TypeBlog Post...and resources References Hall, M. P., Unch, J., Binkowski, B. F., Valley, M. P., Butler, B. L., Wood, M.... -
A Practical Guide to Optimizing AAV DIO and FLEx Vector Expression
TypeBlog Post...a fair amount of time, and it may be tempting to skip some steps and get to experiments. However, by optimizing... -
With an Eye Towards the Future, We Look Back at the March for Science
TypeBlog Post...NIH budget were proposed. I spent a lot of time asking why. As scientists we spend years being taught ... -
Which Fluorescence Microscopy Technique is Best for Me?
TypeBlog Post...microscope to use for a new sample I always start by asking two questions: 1) Is the sample dynamic or static... -
Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins
TypeBlog Post...:18658221. PubMed Central PMCID:PMC2527278. 9.Karbowski, Mariusz, et al. "Quantitation of mitochondrial... -
Antibodies 101: Introduction to Antibodies
TypeBlog Post...green), CARD14 (red), and pNFkB (blue) in lesional skin from a psoriasis patient, showing an inflammatory... -
Live and Let Dye: Self-Labeling Protein Tags
TypeBlog Post...10.1038/s41467-023-39655-3 Wilhelm, J., Kühn, S., Tarnawski, M., Gotthard, G., Tünnermann, J., Tänzer, T.,... -
Deep Dive: qPCR
TypeBlog Post...so pick one and use it! Tempting as it may be to skip this step, remember we here at Addgene frown upon... -
Adeno-associated virus (AAV) Guide
TypeGuide...occur with contact to mucous membranes or broken skin. Needle sticks and ripped gloves are common points... new window) McCarty, D. M., Monahan, P. E., & Samulski, R. J. (2001). Self-complementary recombinant ...Link opens in a new window) Wu, Z., Asokan, A., & Samulski, R. J. (2006). Adeno-associated virus serotypes... -
CRISPR Guide
TypeGuide... 556 (7699), 57–63. PMID: 29512652 Jinek, M., Chylinski, K., Fonfara, I., Hauer, M., Doudna, J. A., & ... C. P., Bonetti, C., Vidigal, J. A., Han, Y., Ogrodowski, P., Crippa, A., Rekhtman, N., De Stanchina, ..., M. T. N., Ioannidi, E. I., Schmitt-Ulms, C., Krajeski, R. N., Lim, J., Villiger, L., Zhou, W., Jiang... -
Viral Vectors 101: Systemic Capsids
TypeBlog Post...Chen, X., Wolfe, D. A., Bindu, D. S., Zhang, M., Taskin, N., Goertsen, D., Shay, T. F., Sullivan, E. E....