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  1. How to Lead a Great Meeting

    Type
    Blog Post
    ...impacted by a project. Instead, invite the experts on the topic at hand. Invite one “ambassador” from each ...

  6. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    ... inserted GFP. After characterizing iGluSnFR in vitro, showing that it is selectively activated by glutamate...they showed that iGluSnFR could detect neuronal activity in C. elegans, zebrafish, and mice. Second Generation...

  9. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    ... continue to be useful for monitoring neuronal activity in the form of calcium dynamics. (For more details...fluorescence) and is useful for imaging signaling activity in large populations of densely-labeled neurons...

  10. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    ... including their potential toxicity and cross-reactivity with other pathways. Since these effectors are...chromosomally. Leakiness was also an issue; some promoter activity persisted in the inactive state. The dynamic range...

  11. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    ... instead of having all the pages in a Curricula Vitae to showcase your publications, academic performances...perhaps you were involved in some extracurricular activities – did you volunteer to do science outreach and...

  13. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ...existing components are adapted and combined with creativity and feedback from the community to create state-of-the-art...Madagascar. Platforms that allow for on-going creativity with the construction plans. Appropriate open-source...

  14. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    .... Envelope gene (may be pseudotyped to alter infectivity): The use of the VSV-G envelope provides the ...tropism envelope protein; Pseudotyped to alter infectivity. Packaging Gag Precursor structural protein...

  15. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ...probes, with sufficient signal for in vivo and in vitro imaging. Saito et al. used this Nano-lantern to ...intravenously with coelenterazine, with increased sensitivity and faster imaging compared to previous publications...

  16. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ...showing that Cpf1 can cleave an array of 4 crRNAs in vitro and when expressed in 293 cells. This allows for...effective method with multiple applications (in vitro, in vivo, transfection, viral transduction) for ...

  17. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    ...commonly employed mismatch nuclease assays. Sensitivity: Less than 20 ng of total gDNA is sufficient ...Findlay, S. D., Vincent, K. M., Berman, J. R. & Postovit, L.-M. A Digital PCR-Based Method for Efficient...

  18. A Deep Dive into BioBuilder

    Type
    Blog Post
    ...How do I engage my students in real-world lab activities? I’m trying to inspire them and also keep up ...extracts to perform transcription and translation in vitro, and the new BioBuilder module allows students to...

  19. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ...work. After all, as we like to sayat Addgene, productivity is infectious. Curious which viruses researchers...mCherry can be used to induce neuronal silencing in vitro and in vivo (Krashes et al., 2011). The various ...

  20. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    ...All_in_one_CRISPR/Cas9_LacZ, a CRISPR gRNA plasmid from Lynne Postovit’s lab. It contains an ampicillin resistance marker...the lacZ gene, thus disrupting 𝛽-galactosidase activity. These cells, presumably the cell you want, cannot...
Showing: 281 - 300 of 576 results