We narrowed to 345 results for: ada

1. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

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   ...colocalization analysis example is given in Figure 2 (adapted from 4) - Dunn et al. 2011 provides an in-depth...

2. Truncated gRNAs for Regulating Gene Expression

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   ...interacts with a ≤15nt gRNA that contains an RNA adapter capable of recruiting a transcriptional activator...

3. Plasmids 101: Knockout/Knock-In Plasmids

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   ...them by depositing with Addgene! Figures were adapted from: Hall B, A Limaye, & AB Kulkarni. (2009) "...

4. Experimenting with New Careers while in Grad School

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   ...online science communicators like Phil Plait @BadAstronomer, Emily Graslie from The Brain Scoop YouTube ...

5. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

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   ...medicine. The parts we have right now are well adapted to work in prokaryotic bacteria, not in fungal ...

6. CRISPR 101: Ribonucleoprotein (RNP) Delivery

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   ...are quickly cleared from the cell via protein degradation pathways. This makes Cas9 RNPs useful for CRISPR...

7. AAVs in Retinal Gene Therapy

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   ...efficiently transduce various types of retinal neurons. Nowadays a toolbox of naturally occurring and engineered...

8. SciComm: Taking Science to Elementary and Middle Schools

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   ...formulated the presentation as follows (which could be adapted for any topic--not just CRISPR): Who am I? Where...

9. Capturing a Moment with Marc Zimmer’s “The State of Science”

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   ...difficulties in dealing with climate change, the degradation of the U.S. environment, and a decline in the...

10. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

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    ...given state or point in time. You want to halt degradative processes while maintaining the structure of ...

11. Getting to Know Expansion Microscopy

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    ...model systems, paired with SRM techniques, and adapted for detecting nucleic acids as well as proteins...

12. A Guide to Starting Your Own Journal Club

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    ...group and topic. For example, it may be useful to adapt the second version (multiple presenter, figure by...

13. The Importance of a Fun Workplace: Company Culture at Addgene

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    ...fashion. 9. Ask for help My grandma used the old adage “many hands make light work” all the time. She was...

14. Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

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    ...immunoprecipitation (enChIP). Fujita T, Asano Y, Ohtsuka J, Takada Y, Saito K, Ohki R, Fujii H. Sci Rep. 2013 Nov ...

15. Plasmids 101: Codon usage bias

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    ...structures in 10 closely related yeast strains. Adaptation to changing conditions - Organisms often need...

16. Neuronal labeling with Spaghetti Monster

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    ...smFPs in the future. References 1. Viswanathan, Sarada, et al. "High-performance probes for light and ...

17. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

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    ...Genetic compensation triggered by mutant mRNA degradation." Nature 568.7751 (2019): 193. PubMed PMID: 30944477...

18. An Inside Look into Addgene's Dev Team

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    ...in iterative chunks, we have the flexibility to adapt and easily respond to changing needs of the teams...

19. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

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    ...pages. We guarantee this will save you a lot of headaches down the line! Cash in on gene synthesis Before...

20. Antibodies 101: The Four ELISAs and When to Use Them

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    ...measure antibody levels, scientists have since adapted it for a host of different proteins and small molecules...