We narrowed to 401 results for: caps

1. Supporting Rare Disease Research with the 2020 BeHEARD Awards

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   Blog Post

   ...patients with GNB1 mutations, the lab hopes to capture the signalosome of different receptors upon stimulation...

2. Antibodies 101: Conjugation

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   ...-based assays. Biotin itself is not visible or capable of producing a visible signal, but it has a strong...

3. To Each HIS Own

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   ... level, try a longer histidine tag to increase capture. Longer histidine tags are also recommended if ...

4. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

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   ... delivery mechanism, but their small packaging capacity of 4-5 kb limits the delivery of larger transgenes...

5. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

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   ... the aforementioned methods only determine the capacity of homodimerization in aqueous solution. In cyto...

6. Developing Lab Management Software for Biology

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   ...materials make everyone's life easier, the time and capital cost of getting up to speed with a setup is almost...

7. Gendered Innovations: Why Does Sex of the Cell Matter?

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   ...that there are sex differences in the therapeutic capacity of stem cells. Yet, very few researchers consider...

8. Plasmids 101: Golden Gate Cloning

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   ...precision cloning method with high throughput capability. Engler C, Kandzia R, Marillonnet S. PLoS One...

9. Career Coaching for Scientists: Why and Where Do I Find One?

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   ...transition is also an area of specialty for Michelle Capes at Adeptify Me. While based in Madison, Wisconsin...

10. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

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    ...fused to CBP, an histone acetyltransferase domain capable of rearranging chromatin structure. This method...

11. How to Prepare for an Industry Interview

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    ...a theme too, which is something not inherently captured by the STAR method. I like to think of the theme...

12. Making CRISPR Plasmids Using Fragmid

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    ...Sizes of fragments are not to scale. Figure and caption from McGee et al., 2024, used under the Creative...

13. Antibodies 101: Stripping and Reprobing Western Blots

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    ...Buffer SDS 2% (w/v) Detergent Β-mercaptoethanol (βME) 100–115 mM = 0.7%–0.8% (v/v) Breaks...

14. Plasmids 101: Modular Cloning

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    ...has been used to assemble multigene constructs capable of performing production and subsequent necessary...

15. Split Fluorescent Proteins for Studying Protein-Protein Interactions

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    ...Description Article PI Blue EBFP2 EBFP2(1-10) and Capri(1-10) for use with GFP(11) Multiplexed labeling ...

16. Design Tips for Prime Editing

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    ...but this also allows certain unintended edits to escape cellular surveillance. To limit unintended incorporation...

17. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

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    ...synthetic polyadenylation (spA) signal. Figure and caption adapted from Hazan and Bester 2021.    Alternative...

18. CRISPR in the Clinic

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    ...Clinical trials are underway to explore CRISPR’s capacity for both classes of edits in cancer therapy. Blood...

19. Viral Vectors 101: Calcium Sensors

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    ...studying the brain, muscles, or intracellular landscape, remember: Keep Ca+2lm and Ca+2rry on! References...

20. Viral Vectors 101: Chemogenetics

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    ...expressed in the brain, it is necessary to use ligands capable of crossing the blood-brain barrier.  Another consideration...