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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 8 results for: caps

Showing: 1 - 8 of 8 results

  1. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...Rep68, Rep52, and Rep40. Cap in trans The cap gene encodes three AAV viral capsid proteins VP1, VP2, and...Packaging plasmid (also known as Rep/Cap plasmid) — containing the Rep and Cap regions necessary for the production...replication and viral packaging. The Cap region encodes three structural capsid proteins or viral proteins (VP1...production, as it carries the Cap region responsible for the production of capsid proteins. AAV2 is the most...to create and test capsid libraries with random mutations or insertions in the Cap region. Several methods...and consists of two open reading frames, Rep and Cap, flanked by two 145 bp inverted terminal repeats ...constructs are designed by replacing the Rep and Cap regions between the two ITRs with a gene expression...

  2. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...transcript begins at the beginning of R, has a 5' cap structure, and proceeds through U5 and the rest of...retroviral particle containing matrix, capsid, and nucleocapsid components. pol in trans Precursor protein...and gamma-retroviruses is that lentiviruses are capable of infecting non-dividing and actively dividing...shRNA of interest flanked by LTRs; ~8 kb packaging capacity Packaging plasmid — contains packaging genes gag...) in cis RNA packaging signal; recognized by nucleocapsid proteins and essential for efficient viral packaging...

  3. Lentiviral Vector Guide

    Type
    Guide
    ...begins, by definition, at the beginning of R, is capped, and proceeds through U5 and the rest of the provirus...lentiviral particle containing matrix, capsid, and nucleocapsid components. pol in trans Precursor protein...and gamma-retroviruses is that lentiviruses are capable of infecting non-dividing and actively dividing...of interest flanked by LTRs; 8–10 kb packaging capacity Packaging plasmid — contains packaging genes gag...) in cis RNA packaging signal; recognized by nucleocapsid proteins and essential for efficient viral packaging...

  4. Antibody Guide

    Type
    Guide
    ...antibodies are capable of recognizing and binding to epitopes on antigens, they are also capable of recognizing...In a sandwich ELISA, a capture antibody, bound to a surface, is used to capture the protein of interest...also be included. Antibody Applications - Capture Methods Capture methods such as immunoprecipitation (IP...variation of IP uses antibodies to capture protein:DNA complexes. After capture, the DNA is released from the...fragmentation of the DNA. Controls for capture methods Capture methods should use a positive control and...methods. Capture MS validation This method uses peptide identification by proteomics (Capture MS) to validate...Amplification Application Overview Quantification Methods Capture Methods Visualization Methods Cell Sorting Methods...

  5. Adenovirus Guide

    Type
    Guide
    ...viral particle and are only composed of a protein capsid and the viral genetic material inside. Adenoviral...structural proteins needed to assemble icosahedral capsids and build new virions. Figure 1: Wild-type adenovirus...physical space available inside of the adenoviral capsid and directly correlates to the size of the DNA ...vectors and further increase the transgene packaging capacity up to ~10.5 kb. The deleted E2 and E4 regions ...vectors are also referred to as "gutless" or high capacity AdV (HCAdV) vectors. Here, all viral sequences...of pAdEasy-2 (Addgene #16401) can increase the capacity of the rAdV vector. pAdEasy-2 does not contain...producing viral vectors in combination with foreign capsid proteins. Researchers have further refined the ...

  6. CRISPR Guide

    Type
    Guide
    ... others work to increase Cas9’s proofreading capabilities. No matter the method, increased fidelity enzymes...by the Feng Zhang lab ) combine the large-scale capacity of transposases with the precise DNA-targeting...programmability for different genomic loci, and the capability to detect multiple targets simultaneously. CRISPR...and biotin tags, or an anti-Cas9 antibody. The CAPTURE system, developed by the Jian Xu lab , utilizes... Cas12a also has more condensed multiplexing capabilities , as it can process multiple gRNAs under a single...carrying an ADAR truncation that retain RNA editing capabilities but are small enough to be packaged in AAV particles...two nuclease domains inactivated. This enzyme is capable of cleaving only one strand of target dsDNA. Off-target...

  7. Science Guides

    Type
    Guide
    ... by the immune system, antibodies are proteins capable of highly specific binding to other proteins. Antibody-based...conjugated to a signaling molecule, can be used to capture, quantify, and identify proteins of interest. Read...

  8. Molecular Biology Reference

    Type
    Guide
    ...replicated using the modified bases and a microscope captures the fluorescent color that is emitted each time...
Showing: 1 - 8 of 8 results