We narrowed to 324 results for: cloning

If you're new to behavioral neuroscience, this article will walk you through how to standardize your experiments and chose viral vectors appropriate for your experiment.

New to using CRISPR pooled libraries for your screen? Here, we cover how to choose a CRISPR library, and the steps and tips for a successful CRISPR screen.

Tips on designing primers for PCR

Summary of degron tags along with their applications, advantages, and several commonly used tags.

In our latest Hot Plasmids, we feature new systemic capsids, Cas-Page, an anti-FLAG antibody, and more!

Find bacterial expression plasmids that can be used for protein purification, protein visualization, study of protein interactions, controlled gene expression, reporter plasmids and genome engineering of bacteria.

Learn how you can use HDR and CRISPR to make a knock-in cell line for your research.

Learn about new plasmids and viral preps available at Addgene including a yeast-based tool for protein evolution, fluorescent tools, nanobody purification, new CRISPR tools, and calcium and norepinephrine biosensors.

Find plasmids more easily with the new search feature on Addgene's website! Now, you can set filters to narrow down your plasmid or viral service search.

The yeast two hybrid (Y2H) assay is useful for probing interactions between proteins. Learn more about the Y2H assay and how you can use it in this post.

Addgene's COVID-19 collection of molecular tools and resources available for Coronavirus research.

Learn how Addgene has worked to increase accessibility to our materials and educational resources.

Aptamers are nucleic acid oligos that bind to small molecules to induce their fluorescence and can be used to study RNA localization and dynamics.

Learn how to transform E. coli with your plasmid of interest.

A list of zebrafish genes and associated pairs of zinc finger arrays that can be used to generate ZFNs targeted to a site within that gene.

Learn about the best-in-class collection of AAV enhancers developed by the Allen Institute for Brain Science!

Check out the latest Hot Plasmids, viral vectors, and antibodies in the Addgene repository!

Learn how to use Easi-CRISPR to generate mouse knock-in and conditional allele models.

Standard protocol for performing agarose gel electrophoresis, including tips to improve resolution and separation of bands.

Browse Addgene's collection of ARF family plasmids. ARF family members include regulatory GTPases, GEFs, and GAPs.