We narrowed to 332 results for: cloning
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  TypeCollection...of one clone that are specific to a certain epitope, or polyclonal, meaning there are many clones that ...antibodies. Recombinant antibodies are created by cloning antibody genes from specific hybridoma cells into... bind to any given antigen. A mature B cell can clone itself to enable higher production of a single antibody...where each culture should produce the same antibody clone, or from plasmid-based expression. Monoclonal antibodies...
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"Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science
TypeBlog Post... or q, respectively). These sequences were then cloned into an AAV backbone containing a minimal promoter... - 
  
Plasmids 101: Expressing shRNAs for RNA Interference
TypeBlog Post...McIntyre, G. J., & Fanning, G. C. (2006). Design and cloning strategies for constructing shRNA expression vectors... - 
  
Plasmid Collections
TypeCollection... view all kits. Kit Types Fluorescent Proteins Cloning Genome Engineering Pathways & ORFs Synthetic Biology... - 
  
Bikard Lab - CRISPR Repression Collection
TypeCollection...Dodd, I. B., & Shearwin, K. E. (2013). One-Step cloning and chromosomal integration of DNA. ACS Synthetic... - 
  
COVID-19 Resources
TypeCollection...211756 Anti-SARS-CoV-2 Nucleocapsid Protein [mBG17] Clone mBG17, recombinant mouse monoclonal antibody targeting...211757 Anti-SARS-CoV-2 Nucleocapsid Protein [mBG86] Clone mBG86, recombinant mouse monoclonal antibody targeting... - 
  
Hot Plasmids: Spring 2025
TypeBlog Post...backbone plasmids here! Figure 2: Addgene cloned EGFP into pAG Lenti CMV N-HA Puro (Addgene #236079... - 
  
Easi-CRISPR: Generating Knock-In and Conditional Mouse Models
TypeBlog Post...mouse embryonic stem cells. After selecting for clones with the recombined product, you’d microinject ... - 
  
DNA Quantification
TypeProtocol...Information During several different stages of molecular cloning, it is important to get a quick and accurate reading... - 
  
Protocol - How to Design Primers
TypeProtocol...also like... Polymerase Chain Reaction Plasmid Cloning by PCR Agarose Gel Electrophoresis Primer Design... - 
  
Retrovirus Plasmids
TypeCollection...pMSCV PIG (Puro IRES GFP empty plasmid) MSCV For cloning and gene expression. Select with puromycin or screen... - 
  
Zinc Finger Consortium: Zinc Finger Arrays
TypeCollection...vectors, DNA encoding zinc finger arrays should be cloned into plasmids also available through Addgene--the... - 
  
Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands
TypeBlog Post...self-labeling protein fusions as well as empty backbones to clone your own protein of interest. Fluorescence microscopy... - 
  
Cancer and the Immune System: Deciphering the Relationship
TypeBlog Post...Cancer Drug Resistance Checkout the RAS Pathway Clone Collection ... - 
  
Antibodies 101: Flow Cytometry
TypeBlog Post... you spent hours in the biosafety cabinet with cloning rings or following labor-intensive limiting dilution... - 
  
Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein
TypeBlog Post... alleles may be warranted (20-30% of all tagged clones were bi-allelic in our hands). Take-home message... - 
  
Bacterial Expression Systems
TypeCollection...purification tags compatible with Golden Gate modular cloning (MoClo) for use in E. coli . Addgene Blog E. Coli...microscope. The plasmids in this collection are clone-in ready plasmids that contain fluorescent protein... - 
  
Arf GTPase Family
TypeCollection...ARF Superfamily Research You may also like... RAS Clone Collections Richard Kahn Lab Plasmids GTPase Plasmids... - 
  
TALEN Guide
TypeCollection...be used with the recently developed Golden Gate cloning method to assemble “multiple DNA fragments in an... - 
  
Zebrafish Plasmid Collection
TypeCollection...with fluorescent proteins to visualize and track clones of cells over time. Examples of useful tools for...