We narrowed to 329 results for: cloning
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TypeProtocol... Protocols Cloning by Limiting Dilution Isolating a Monoclonal Cell...transgene expression over time, as the lower expressing clones take over the polyclonal cell pool. Generating ...new window) or isolating individual cells with cloning cylinders. Workflow Timeline This protocol begins...
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Bikard Lab - CRISPR Repression Collection
TypeCollection...Dodd, I. B., & Shearwin, K. E. (2013). One-Step cloning and chromosomal integration of DNA. ACS Synthetic... -
"Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science
TypeBlog Post... or q, respectively). These sequences were then cloned into an AAV backbone containing a minimal promoter... -
Plasmids 101: Expressing shRNAs for RNA Interference
TypeBlog Post...McIntyre, G. J., & Fanning, G. C. (2006). Design and cloning strategies for constructing shRNA expression vectors... -
COVID-19 Resources
TypeCollection... Anti-SARS-CoV-2 Nucleocapsid Protein [mBG17] - Clone mBG17, recombinant mouse monoclonal antibody targeting... Anti-SARS-CoV-2 Nucleocapsid Protein [mBG86] - Clone mBG86, recombinant mouse monoclonal antibody targeting... -
Easi-CRISPR: Generating Knock-In and Conditional Mouse Models
TypeBlog Post...mouse embryonic stem cells. After selecting for clones with the recombined product, you’d microinject ... -
Hot Plasmids: Spring 2025
TypeBlog Post...backbone plasmids here! Figure 2: Addgene cloned EGFP into pAG Lenti CMV N-HA Puro (Addgene #236079... -
DNA Quantification
TypeProtocol...Information During several different stages of molecular cloning, it is important to get a quick and accurate reading... -
Retrovirus Plasmids
TypeCollection...pMSCV PIG (Puro IRES GFP empty plasmid) MSCV For cloning and gene expression; select with puromycin or screen... -
Protocol - How to Design Primers
TypeProtocol...also like... Polymerase Chain Reaction Plasmid Cloning by PCR Agarose Gel Electrophoresis Primer Design... -
Zinc Finger Consortium: Zinc Finger Arrays
TypeCollection...vectors, DNA encoding zinc finger arrays should be cloned into plasmids also available through Addgene--the... -
Cancer and the Immune System: Deciphering the Relationship
TypeBlog Post...Cancer Drug Resistance Checkout the RAS Pathway Clone Collection ... -
Antibodies 101: Flow Cytometry
TypeBlog Post... you spent hours in the biosafety cabinet with cloning rings or following labor-intensive limiting dilution... -
Bacterial Expression Systems
TypeCollection...purification tags compatible with Golden Gate modular cloning (MoClo) for use in E. coli . Addgene Blog E. Coli...microscope. The plasmids in this collection are clone-in ready plasmids that contain fluorescent protein... -
Arf GTPase Family
TypeCollection...ARF Superfamily Research You may also like... RAS Clone Collections Richard Kahn Lab Plasmids GTPase Plasmids... -
TALEN Guide
TypeCollection...be used with the recently developed Golden Gate cloning method to assemble “multiple DNA fragments in an... -
Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein
TypeBlog Post... alleles may be warranted (20-30% of all tagged clones were bi-allelic in our hands). Take-home message... -
Zebrafish Plasmid Collection
TypeCollection...with fluorescent proteins to visualize and track clones of cells over time. Examples of useful tools for... -
CRISPR Plasmids - Mammalian Expression
TypeCollection... based on factors such as selectable marker or cloning method. To isolate mammalian vectors, simply type... -
Deep Dive: qPCR
TypeBlog Post...Digital Droplet PCR for AAV QuantificationPlasmid Cloning by PCR...