... 101: How to Verify Your Plasmid Plasmids 101: Control Plasmids 6 Tips for Analyzing and Troubleshooting...

...your plasmids, storing them, providing quality control and customer support, making plasmids easy for ...

... of knockdown and developed new, more tightly controlled, Tet responsive promoters to avoid leaky activation...This approach provides a more granular level of control where individual bases can be modified. Cytidine...as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression. Cell 152:1173–1183 . https:...

...significant hit across the E. coli genome as a negative control. Read the paper in Nature Communications Find...

...has the advantage that CRISPR efficiency can be controlled by manipulating complementarity between the gRNA...

...noises and flashing lights - that sent average, control mice into depression. In case you were wondering...

...circuits, allowing for tighter spatio-temporal control of gene expression within a cell. Thanks to the...

... and experimental resources that relate to the control of gene expression in the grasses. OpenPlant Synthetic...

...vectors to express your gene of interest under the control of a variety of promoters. Professor Oka and the...

...-occurring Acr (Anti-CRISPR) proteins can help control CRISPR activity. Acr proteins have evolved in phages...LmoCas9 and SpCas9. Acr proteins can be used to control both when and where genome editing happens. CASANOVA...Dominik Niopek’s lab , is a way to optogenetically control genome editing by fusing the anti-CRISPR protein...photosensitive LOV2 domain . Several labs have also controlled Acr activity with endogenous cell-type specific...have also been used in in vivo RNA imaging or to control alternative splicing. Figure 13: Overview of RNA...Engineered anti-CRISPR proteins for optogenetic control of CRISPR–Cas9. Nature Methods , 15 (11), 924–927...homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery. eLife . 3 e04766...

...with the lentivirus HIV-1. Today, HIV-1 can be controlled with antiviral therapies such that the virus ...infection period rather than the ART-induced viral control found in most HIV-1 patients. Obstacles for CRISPR...

... they do so by activating signaling pathways controlling properties like growth, survival, and differentiation...

...TOPFlash, scramble shRNA for use as a negative control, and empty backbones such as pBABE-puro for retroviral...

...effect on cell viability compared to a wild-type control. Approximately ~22% of the mutations, however, ...

...technique from David Liu’s lab that gives more control and flexibility to CRISPR editing. Using a fusion...

...documents from anywhere, but also allow you to control who can edit the documents and track the revision...

...protein and a tag, such as an NLS or NES, enables control over localization. Inserting PhoCl between an enzyme...enzyme and an inhibitor domain similarly allows for control of enzymatic activity. In addition, proteins can...expression. Expression of toolkit components can be controlled by a variety of promoters for versatile and robust...

...Epigenetic modifications are an additional layer of control over gene expression that go beyond genomic sequence...catalytically dead modifiers are also available as controls. For an up-to-date list of CRISPR epigenetic tools...

...the number of integration copies can be tightly controlled by titrating the transposase to transposon ratios... levels of your gene of interest, as needed. Controlling the expression levels of your gene may be particularly...

...FRT, with AAV vectors are common approaches to control transgene expression by restricting expression ...